MALDI-TOF characterization of NAPPA-generated proteins

Journal of Mass Spectrometry

Volumn 46, Issue 9, 2011, Pages 960-965

  Spera, Rosanna ^a   Labaer, Joshua ^b   Nicolini, Claudio ^a  

^a UNIVERSITY OF GENOA   (Italy)

^b HARVARD UNIVERSITY   (United States)

Author keywords

label free analysis; MALDI TOF MS; mass spectrometry; NAPPA; protein microarray

Indexed keywords

BOVINE SERUM ALBUMIN; COMPLEMENTARY DNA; GLUTATHIONE TRANSFERASE; HYBRID PROTEIN; PLASMID DNA; TRYPSIN;

AMINO ACID SEQUENCE; BIOINFORMATICS; DENATURATION; GENETIC TRANSCRIPTION AND TRANSLATION; IMMOBILIZATION; LETTER; MATRIX ASSISTED LASER DESORPTION IONIZATION TIME OF FLIGHT MASS SPECTROMETRY; NUCLEIC ACID PROGRAMMABLE PROTEIN ARRAY; PRIORITY JOURNAL; PROTEIN EXPRESSION; PROTEIN MICROARRAY; PROTEIN STABILITY; PROTEIN TARGETING;

AMINO ACID SEQUENCE; DNA, COMPLEMENTARY; HUMANS; MOLECULAR SEQUENCE DATA; PROTEIN ARRAY ANALYSIS; PROTEINS; SPECTROMETRY, MASS, MATRIX-ASSISTED LASER DESORPTION-IONIZATION; TUMOR SUPPRESSOR PROTEIN P53;

EID: 80052809420     PISSN: 10765174     EISSN: 10969888     Source Type: Journal    
DOI: 10.1002/jms.1976     Document Type: Letter

References (11)

1. V. Sakanyan, High throughput and multiplexed protein array technology: protein-DNA and protein-protein interactions. J. Chromatogr. B 2005, 815, 77.
2. N. Ramachandran, E. Hainsworth, B. Bhullar, S. Eisenstein, B. Rosen, A. Y. Lau, J. C. Walter, J. LaBaer,. Self-assembling protein microarrays. Science 2004, 305, 86.
3. N. Ramachandran, D. N. Larson, P.R. Stark, E. Hainsworth, J. LaBaer,. Emerging tools for real-time label-free detection of interactions on functional protein microarrays. FEBS J. 2005, 272, 5412.
4. R. Spera, C. Nicolini,. NAPPA microarrays and mass spectrometry: new trends and challenges. Nano Article-Taylor & Francis/CRC Press, California, 2008.
5. S. Nagl, M. Schaeferling, O. S. Wolfbeis,. Fluorescence analysis in microarray technology. Microchimica Acta 2005, 151, 1.
6. N. Ramachandran, J. V. Raphael, E. Hainsworth, G. Demirkan, M. G. Fuentes, A. Rolfs, Y. Hu, J. LaBaer,. Next-generation high-density self-assembling functional protein arrays. Nat. Methods 2008, 5, 535.
7. J. O. Koopmann, J. Blackburn,. High affinity capture surface for matrix-assisted laser desorption/ionisation compatible protein microarrays. Rapid Commun. Mass Spectrom. 2003, 17, 455.
8. D. Finnskog, A. Ressine, T. Laurell, G. Marko-Varga,. Integrated protein microchip assay with dual fluorescent and MALDI read-out. J. Proteome Res. 2004, 3, 988.
9. D. J. Pappin, P. Hojrup, A. J. Bleasby,. Rapid identification of proteins by peptide-mass fingerprinting. Curr. Biol. 1993, 3, 327.
10. Xu H., and, Freitas M. A., A mass accuracy sensitive probability based scoring algorithm for database searching of tandem mass spectrometry data, BMC Bioinformatics 2007, 8: 133.
11. Pechkova E., Chong S., Tripathi S., Nicolini C., Cell free expression and APA for NAPPA and protein crystallography, in Functional Proteomics and Nanotechnology-based Microarrays (Eds., Nicolini C., LaBaer J.,) Pan Stanford Series on Nanobiotechnology Volume 2, Chapter 7, pp. 121-147, London-New York-Singapore, 2010.