High-throughput target-selected gene inactivation in zebrafish

Methods in Cell Biology

Volumn 104, Issue , 2011, Pages 121-127

  Kettleborough, Ross N W ^a   Bruijn, Ewart de ^b   Eeden, Freek van ^c   Cuppen, Edwin ^b   Stemple, Derek L ^a  

^a WELLCOME TRUST SANGER INSTITUTE   (United Kingdom)

^b UNIVERSITY MEDICAL CENTER UTRECHT   (Netherlands)

^c UNIVERSITY OF SHEFFIELD   (United Kingdom)

Author keywords

Mutagenesis; Mutation Detection; Reverse Genetics; Sequencing; TILLING; Zebrafish

Indexed keywords

EID: 80052786773     PISSN: 0091679X     EISSN: None     Source Type: Book Series    
DOI: 10.1016/B978-0-12-374814-0.00006-9     Document Type: Chapter

References (11)

1. Cuppen E. Genotyping by Allele-Specific Amplification (KASPar). CSH Protoc 2007 2007, pdb prot4841.
2. Goda T., Abu-Daya A., Carruthers S., Clark M.D., Stemple D.L., Zimmerman L.B. Genetic screens for mutations affecting development of Xenopus tropicalis. PLoS Genet. 2006, 2(6):e91.
3. Gordon D., Abajian C., Green P. Consed: a graphical tool for sequence finishing. Genome Res. 1998, 8(3):195-202.
4. Jansen G., Hazendonk E., Thijssen K.L., Plasterk R.H. Reverse genetics by chemical mutagenesis in Caenorhabditis elegans. Nat. Genet. 1997, 17(1):119-121.
5. McCallum C.M., Comai L., Greene E.A., Henikoff S. Targeted screening for induced mutations. Nat. Biotechnol. 2000, 18(4):455-457.
6. Mullins M.C., Hammerschmidt M., Haffter P., Nusslein-Volhard C. Large-scale mutagenesis in the zebrafish: in search of genes controlling development in a vertebrate. Curr. Biol. 1994, 4(3):189-202.
7. Solnica-Krezel L., Schier A.F., Driever W. Efficient recovery of ENU-induced mutations from the zebrafish germline. Genetics 1994, 136(4):1401-1420.
8. Stephens M., Sloan J.S., Robertson P.D., Scheet P., Nickerson D.A. Automating sequence-based detection and genotyping of SNPs from diploid samples. Nat. Genet. 2006, 38(3):375-381.
9. Till B.J., Burtner C., Comai L., Henikoff S. Mismatch cleavage by single-strand specific nucleases. Nucleic Acids Res. 2004, 32(8):2632-2641.
10. van Eeden F.J., Granato M., Odenthal J., Haffter P. Developmental mutant screens in the zebrafish. Methods Cell Biol. 1999, 60:21-41.
11. Wienholds E., van Eeden F., Kosters M., Mudde J., Plasterk R.H., Cuppen E. Efficient target-selected mutagenesis in zebrafish. Genome Res. 2003, 13(12):2700-2707.