Quantitation of locked nucleic acid antisense oligonucleotides in mouse tissue using a liquid-liquid extraction LC-MS/MS analytical approach

Bioanalysis

Volumn 3, Issue 17, 2011, Pages 1911-1921

  Turnpenny, Paul ^a   Rawal, Jaiessh ^a   Schardt, Torsten ^b   Lamoratta, Sandro ^b   Mueller, Heiko ^b   Weber, Markus ^b   Brady, Kevin ^a  

^a PFIZER GLOBAL RESEARCH AND DEVELOPMENT   (United Kingdom)

^b PFIZER INC   (United States)

Author keywords

[No Author keywords available]

Indexed keywords

ANTISENSE OLIGONUCLEOTIDE; SHORT HAIRPIN RNA;

ACCURACY; ANALYTIC METHOD; ANIMAL TISSUE; ARTICLE; CALIBRATION; CONTROLLED STUDY; INTERMETHOD COMPARISON; LIGAND BINDING; LIQUID CHROMATOGRAPHY; LIQUID LIQUID EXTRACTION; MASS SPECTROMETRY; MOUSE; NONHUMAN; PLASMA HALF LIFE; PROCESS OPTIMIZATION; SENSITIVITY AND SPECIFICITY; SEPARATION TECHNIQUE; TISSUE DISTRIBUTION;

ANIMALS; CHROMATOGRAPHY, LIQUID; KIDNEY; LIVER; MICE; OLIGONUCLEOTIDES, ANTISENSE; REFERENCE STANDARDS; SOLID PHASE EXTRACTION; TANDEM MASS SPECTROMETRY;

EID: 80052724248     PISSN: 17576180     EISSN: 17576199     Source Type: Journal    
DOI: 10.4155/bio.11.100     Document Type: Article

References (14)

1. Wei X, Dai G, Lui Z et al. Metabolism of GTI-2040, a phosphorothioate oligonucleotide antisense, using ion-pair reverse phase high-performance liquid chromatography (HPLC) coupled with electrospray ion-trap mass spectrometry. AAPS J. 8(4), 743-775 (2006).
2. Lin ZJ, Li W, Dai G. Application of LC-MS for quantitative analysis and metabolite identification of therapeutic oligonucleotides. J. Pharm. Biomed. Anal. 44, 330-341 (2007). (Pubitemid 46887636)
3. Yu RZ, Kim TW, Hong A et al. Cross-species pharmacokinetic comparison from mouse to man of a second-generation antisense oligonucleotide. ISIS 301012. Targeting human apolipoprotein B-100. Drug. Metab. Dispos. 35(3), 460-468 (2007). (Pubitemid 46333910)
4. Passos de Jesus R, De Nardi L, Da Ros N et al. Amino acids change liver growth factors gene expression in malnourished rats. Nutricion Hospitalaria 25(3), 382-387 (2010).
5. Zou Y, Tiller P, Chen IW, Beverly M, Hochman J. Metabolite identification of small interfering RNA duplex by high resolution accurate mass spectrometry. Rapid. Comm. Mass Spec. 22, 1871-1881 (2008). (Pubitemid 351947753)
6. Zhang G, Lin J, Srinivasan K, Kavetskaia O, Duncan N. Strategies for bioanalysis of an oligonucleotide class macromolecule from rat plasma using liquid chromatography-tandem mass spectrometry. Anal. Chem. 79, 3416-3424 (2007). (Pubitemid 46717172)
7. Tremblay GA, Oldfield PR. Bioanalysis of siRNA and oligonucleotide therapeutics in biological fluids and tissues. Bioanalysis 1(3), 595-609 (2009).
8. Johnson JL, Guo W, Zang J et al. Quantification of raf antisense oligonucleotide (rafAON) in biological matrices by LC-MS/ MS to support pharmacokinetics of liposome-entrapped rafAON formulation. Bio. Med. Chrom. 19, 272-278 (2005). (Pubitemid 40872655)
9. Bakry R, Huck CW, Bonn GK. Recent applications of organic monoliths in capillary liquid chromatographic separation of biomolecules. J. Chrom. Sci. 47(6), 418-431 (2009).
10. Jackschitz TA, Huck CW, Lubbad S, Bonn GK. Monolithic poly[trimetylsilyl-4-methylstyrene0- co- bis(4-vinylbenzyl)dimethylsilane] stationary phases for the fast separation of proteins and oligonucleotides. J. Chrom. A 1147, 53-58 (2007). (Pubitemid 46428270)
11. Premstaller A, Oberacher H, Huber CG. High-performance liquid chromatography- electrospray ionization mass spectrometry of single- and double-stranded nucleic acids using monolithic capillary columns. Anal. Chem. 72, 4386-4393 (2000).
12. Premstaller A, Oberacher H, Huber CG. Characterization of some physical and chromatographic properties of monolithic poly(styrene-co-divinylbenzene) columns. J. Chrom. A 1030, 201-208 (2004). (Pubitemid 38221330)
13. Higuchi K, Shimoishi Y, Miyata H, Toei K. Spectrophotometric determination of anionic surfactants in river waters using1-(4- nitrobenzyl)-4-(4-diethylaminophenylazo)- pyridinium bromide. Analyst 105, 768-773 (1980). (Pubitemid 10030672)
14. Khym JX. The use of amines in liquid-liquid extractions of nucleic acids and related compounds. Biochemistry 2(3), 401-405 (1963).