Methods to Analyze the Homing Efficiency and Spatial Distribution of Hematopoietic Stem and Progenitor Cells and Their Relationship to the Bone Marrow Endosteum and Vascular Endothelium

Methods in Molecular Biology

Volumn 750, Issue , 2011, Pages 197-214

  Grassinger, Jochen ^b   Nilsson, Susie K ^a  

^a MONASH UNIVERSITY   (Australia)

^b NONE

Author keywords

Bone marrow; Hematopoietic stem cell; Homing; Niche; Vasculature

Indexed keywords

ANIMAL CELL; ANIMAL EXPERIMENT; ANIMAL MODEL; BONE MARROW; HEMATOPOIETIC STEM CELL; HOMING BEHAVIOR; IMMUNOFLUORESCENCE; MICROENVIRONMENT; MURINE MODEL; NONHUMAN; TRANSENDOTHELIAL AND TRANSEPITHELIAL MIGRATION; VASCULAR ENDOTHELIUM; VASCULARIZATION; ANIMAL; ARTICLE; C57BL MOUSE; CELL DIFFERENTIATION; CELL MOTION; CYTOLOGY; FLOW CYTOMETRY; HEMATOPOIETIC STEM CELL TRANSPLANTATION; IMMUNOMAGNETIC SEPARATION; MOUSE; PHYSIOLOGY; STEM CELL NICHE;

5 (6) CARBOXYFLUORESCEIN DIACETATE SUCCINIMIDYL ESTER; 5-(6)-CARBOXYFLUORESCEIN DIACETATE SUCCINIMIDYL ESTER; BENZOPYRAN DERIVATIVE; FLUORESCEIN DERIVATIVE; FLUORESCENT DYE; NAPHTHOL DERIVATIVE; RHODAMINE; SEMINAPHTHORHODAMINEFLUORIDE; SUCCINIMIDE DERIVATIVE;

ANIMALS; BENZOPYRANS; BONE MARROW; CELL DIFFERENTIATION; CELL MOVEMENT; ENDOTHELIUM, VASCULAR; FLOW CYTOMETRY; FLUORESCEINS; FLUORESCENT DYES; HEMATOPOIETIC STEM CELL TRANSPLANTATION; HEMATOPOIETIC STEM CELLS; IMMUNOMAGNETIC SEPARATION; MICE; MICE, INBRED C57BL; NAPHTHOLS; RHODAMINES; STEM CELL NICHE; SUCCINIMIDES; TRANSENDOTHELIAL AND TRANSEPITHELIAL MIGRATION;

EID: 80052612240     PISSN: 10643745     EISSN: 19406029     Source Type: Book Series    
DOI: 10.1007/978-1-61779-145-1_14     Document Type: Chapter

References (12)

1. Calvi, L.M., et al. (2003) Osteoblastic cells regulate the haematopoietic stem cell niche Nature 425, 841–6.
2. Haylock, D.N. and S.K. (2007) Nilsson, Expansion of umbilical cord blood for clinical transplantation Curr Stem Cell Res Ther 2, 324–35.
3. Nilsson, S.K., et al. (2003) Hyaluronan is synthesized by primitive haemopoietic cells, participates in their lodgement at the endosteum following transplantation, and is involved in the regulation of their proliferation and differentiation in vitro Blood 101, 856–62.
4. Nilsson, S.K., et al. (2005) Osteopontin, a key component of the hematopoietic stem cell niche and regulator of primitive hematopoietic progenitor cells. Blood 106, 1232–9.
5. Zhang, J., et al. (2003) Identification of the haematopoietic stem cell niche and control of the niche size Nature 425, 836–41.
6. Kiel, M.J., et al. (2005) SLAM family receptors distinguish hematopoietic stem and progenitor cells and reveal endothelial niches for stem cells Cell 121, 1109–21.
7. Lo Celso, C., et al. (2009) Live-animal tracking of individual haematopoietic stem/progenitor cells in their niche Nature 457, 92–6.
8. Xie, Y., et al. (2009) Detection of functional haematopoietic stem cell niche using real-time imaging Nature 457, 97–101.
9. Haylock, D.N., et al. (2007) Hemopoietic stem cells with higher hemopoietic potential reside at the bone marrow endosteum Stem Cells 25, 1062–9.
10. Williams, B. and S.K. Nilsson. (2009) Investigating the interactions between haemopoietic stem cells and their niche: methods for the analysis of stem cell homing and distribution within the marrow following transplantation Methods Mol Biol 482, 93–107.
11. Nilsson, S.K., et al. (1997) Potential and distribution of transplanted hematopoietic stem cells in a nonablated mouse model Blood 89, 4013–20.
12. Ellis SL, et al. (2008) Defining the HSC Niche: The relationship between bone, HSC and vasculature Exp Hem 36, 61.