Ligand association rates to the inner-variable-domain of a dual-variable-domain immunoglobulin are significantly impacted by linker design

mAbs

Volumn 3, Issue 5, 2011, Pages 487-494

  DiGiammarino, Enrico L ^a   Harlan, John E ^a   Walter, Karl A ^a   Ladror, Uri S ^a   Edalji, Rohinton P ^a   Hutchins, Charles W ^a   Lake, Marc R ^a   Greischar, Amy J ^a   Liu, Junjian ^a   Ghayur, Tariq ^a   Jakob, Clarissa G ^a  

^a ABBOTT LABORATORIES   (United States)

Author keywords

Antibody engineering; Dual variable domain immunoglobulin; Dual specific; DVD Ig; Immunotherapy; Linker; Variable domain

Indexed keywords

DVD IMMUNOGLOBULIN; IMMUNOGLOBULIN; IMMUNOGLOBULIN HEAVY CHAIN; IMMUNOGLOBULIN LIGHT CHAIN; LIGAND; MONOCLONAL ANTIBODY; TUMOR NECROSIS FACTOR ALPHA; UNCLASSIFIED DRUG;

AMINO ACID SEQUENCE; ANTIGEN BINDING; ARTICLE; BINDING AFFINITY; BINDING KINETICS; LIGAND BINDING; PROTEIN CLEAVAGE; PROTEIN DOMAIN; PROTEIN SYNTHESIS;

AMINO ACID SEQUENCE; ANTIBODIES, MONOCLONAL; DRUG DESIGN; HUMANS; IMMUNOGLOBULIN VARIABLE REGION; KINETICS; LIGANDS; MOLECULAR SEQUENCE DATA; PROTEIN BINDING; PROTEIN ENGINEERING; PROTEIN STRUCTURE, TERTIARY; TUMOR NECROSIS FACTOR-ALPHA;

EID: 80052580966     PISSN: 19420862     EISSN: 19420870     Source Type: Journal    
DOI: 10.4161/mabs.3.5.16326     Document Type: Article

References (11)

1. Enever C., Batuwangala T., Plummer C., Sepp A. Next generation immunotherapeutics - Honing the magic bullet. Curr Opin Biotechnol 2009; 20:405-411
2. Uno T., Takeda K., Kojima Y., Yoshizawa H., Akiba H., Mittler R.S., et al. Eradication of established tumors in mice by a combination antibody-based therapy. Nat Med 2006; 12:693-698 (Pubitemid 43865240)
3. Wu C., Ying H., Grinnell C., Bryant S., Miller R., Clabbers A., et al. Simultaneous targeting of multiple disease mediators by a dual-variable-domain immunoglobulin. Nat Biotechnol 2007; 25:1290-1297 (Pubitemid 350076510)
4. Wu C., Ying H., Bose S., Miller R., Medina L., Santora L., et al. Molecular construction and optimization of antihuman IL-1alpha/beta dual variable domain immunoglobulin (DVD-Ig) molecules. MAbs 2009; 1:339-347
5. Wu C., Ghayur T., Salfeld J. Generation and Characterization of a Dual Variable Domain Immunoglobulin (DVD-Ig™) Molecule in Kontermann R, Dübel S, Eds. Antibody Engineering 2010; 2:239-50.
6. Andrew S.M., Titus J.A. Fragmentation of immunoglobulin G. Curr Protoc Cell Biol 2003; 16:4; PMID: 18228421.
7. Goding J.W. Purification, fragmentation and isotopic labeling of monoclonal antibodies in Monoclonal Antibodies: Principles and Practice (third edition), Academic Press, San Diego CA 1996; 192-233.
8. Parham P. On the fragmentation of monoclonal IgG1, IgG2a and IgG2b from BALB/c mice. J Immunol 1983; 131:2895-2902 (Pubitemid 14208140)
9. Myszka D.G. Improving biosensor analysis. J Mol Recognit 1999; 12:279-284 (Pubitemid 129708590)
10. Sandin S., Ofverstedt L.G., Wikström A.C., Wrange O., Skoglund U. Structure and flexibility of individual immunoglobulin G molecules in solution. Structure 2004; 12:409-415 (Pubitemid 38353062)
11. Evans E.J., Esnouf R.M., Manso-Sancho R., Gilbert R.J., James J.R., Yu C., et al. Crystal structure of a soluble CD28-Fab complex. Nat Immunol 2005; 6:271-279 (Pubitemid 41724764)