Optimized assay and storage conditions for enzyme activity profiling of ectomycorrhizae

Mycorrhiza

Volumn 21, Issue 7, 2011, Pages 589-600

  Pritsch, Karin ^a   Courty, Pierre Emanuel ^c,d   Churin, Jean Louis ^c   Cloutier Hurteau, Benoit ^g   Ali, Muhammad Arif ^g   Damon, Coralie ^f   Duchemin, Myriam ^g   Egli, Simon ^e   Ernst, Jana ^a   Fraissinet Tachet, Laurence ^f   Kuhar, Francisco ^a,b   Legname, Elvira ^g   Marmeisse, Roland ^f   Müller, Alex ^e   Nikolova, Petia ^a   Peter, Martina ^e   Plassard, Claude ^g   Richard, Franck ^h   Schloter, Michael ^a   Selosse, Marc André ^h   Franc, Alain ⁱ   Garbaye, Jean ^c   more..

^a INSTITUTE OF EPIDEMIOLOGY   (Germany)

^b UNIVERSIDAD DE BUENOS AIRES   (Argentina)

^c UNIVERSITÉ DE LORRAINE   (France)

^d UNIVERSITY OF BASEL   (Switzerland)

^e SWISS FEDERAL RESEARCH INSTITUTE WSL   (Switzerland)

^f University of Lyon   (France)

^g UMR ECO SOLS   (France)

^h CENTRE D ECOLOGIE FONCTIONNELLE ET EVOLUTIVE   (France)

ⁱ UMR 1202 BIOGECO   (France)

Author keywords

Ectomycorrhizae; Enzyme activity profiling; Method optimization; Sample storage

Indexed keywords

ENZYME;

BIOASSAY; ECTOMYCORRHIZA; ENZYME ACTIVITY; ERROR ANALYSIS; ETHANOL; FILTER; FREEZING; GENETIC ANALYSIS; MORPHOTYPE; OPTIMIZATION; PRESERVATION; RESEARCH WORK;

ARTICLE; COLD; ENZYMOLOGY; METHODOLOGY; MICROBIOLOGICAL EXAMINATION; MICROBIOLOGY; MYCOLOGY; MYCORRHIZA; PLANT ROOT; PRESERVATION; TIME;

COLD TEMPERATURE; ENZYMES; MICROBIOLOGICAL TECHNIQUES; MYCOLOGY; MYCORRHIZAE; PLANT ROOTS; PRESERVATION, BIOLOGICAL; TIME FACTORS;

EID: 80052540871     PISSN: 09406360     EISSN: None     Source Type: Journal    
DOI: 10.1007/s00572-011-0364-4     Document Type: Article

References (41)

1. Abuzinadah RA, Read DJ (1989) The role of proteins in the nitrogen nutrition of ectomycorrhizal plants. V. Nitrogen transfer in birch (Betula pendula) grown in association with mycorrhizal and non-mycorrhizal fungi. New Phytol 112: 61-68.
2. Agerer R (1991) Characterization of ectomycorrhiza. Methods Microbiol 23: 25-73.
3. Agerer R (2001) Exploration types of ectomycorrhizae. Mycorrhiza 11: 107-114.
4. Bernard M, Mouyna I, Dubreucq G, Debeaupuis J-P, Fontaine T, Vorgias C, Fuglsang C, Latgé J-P (2002) Characterization of a cell-wall acid phosphatase (PhoAp) in Aspergillus fumigatus. Microbiology 148: 2819-2829.
5. Buée M, Vairelles D, Garbaye J (2005) Year-round monitoring of diversity and potential metabolic activity of the ectomycorrhizal community in a beech (fagus silvatica) forest subjected to two thinning regimes. Mycorrhiza 15: 235-245.
6. Buee M, Courty PE, Mignot D, Garbaye J (2007) Soil niche effect on species diversity and catabolic activities in an ectomycorrhizal fungal community. Soil Biol Biochem 39: 1947-1955.
7. Chróst RJ, Velimirov B (1991) Measurement of enzyme kinetics in water samples: effect of freezing and soluble stabilizer. Mar Ecol Prog Ser 70: 93-100.
8. Cosby CN, Troiano NW, Kacena MA (2008) The effects of storage conditions on the preservation of enzymatic activity in bone. J Histotechnol 31: 169-173.
9. Courty P-E, Pritsch K, Schloter M, Hartmann A, Garbaye J (2005) Activity profiling of ectomycorrhiza communities in two forest soils using multiple enzymatic tests. New Phytol 167: 309-319.
10. Courty P-E, Breda N, Garbaye J (2007) Relation between oak tree phenology and the secretion of organic matter degrading enzymes by Lactarius quietus ectomycorrhizas before and during bud break. Soil Biol Biochem 39: 1655-1663.
11. Courty PE, Franc A, Garbaye J (2010) Temporal and functional pattern of secreted enzyme activities in an ectomycorrhizal community. Soil Biol Biochem 42: 2022-2025.
12. Courty PE, Labbe J, Kohler A, Marçais B, Bastien C, Churin JL, Garbaye J, Le Tacon F (2011) Effect of poplar genotypes on mycorrhizal infection and secreted enzyme activities in mycorrhizal and non-mycorrhizal roots. J Exp Bot 62: 249-260.
13. De Bolle X, Vinals C, Fastrez J, Feytmans E (1997) Bivalent cations stabilize yeast alcohol dehydrogenase I. Biochem J 323: 409-413.
14. Duponnois R, Garbaye J (1991) Techniques for controlled synthesis of the Douglas-fir-Laccaria laccata ectomycorrhizal symbiosis. Ann For Sci 48: 641-650.
15. Hedh J, Wallander H, Erland S (2008) Ectomycorrhizal mycelial species composition in apatite amended and non-amended mesh bags buried in a phosphorus-poor spruce forest. Mycol Res 112: 681-688.
16. Hoppe H-G (1983) Significance of exoenzymatic activities in the ecology of brackish water: measurements by means of methylumbelliferyl-substrates. Mar Ecol Prog Ser 11: 299-308.
17. Horton TR, Bruns TD (2001) The molecular revolution in ectomycorrhizal ecology: peeking into the black-box. Mol Ecol 10: 1855-1871.
18. Kacena MA, Troiano NW, Coady CE, Horowitz MC (2004) Effects of ethanol post-fixation on the histological, histochemical, and immunohistochemical analysis of murine bone specimens. J Histotechnol 27: 15-20.
19. Latgé J-P (2007) The cell wall: a carbohydrate armour for the fungal cell. Mol Microbiol 66: 279-290.
20. Lehto T, Brosinsky A, Heinonen-Tanski H, Repo T (2008) Freezing tolerance of ectomycorrhizal fungi in pure culture. Mycorrhiza 18: 385-392.
21. Martin F, Selosse M-A (2008) The Laccaria genome: a symbiont blueprint decoded. New Phytol 180: 296-310.
22. Martin F, Kohler A, Murat C, Balestrini R, Coutinho PM, Jaillon O, Montanini B, Morin E, Noel B, Percudani R, Porcel B, Rubini A, Amicucci A, Amselem J, Anthouard V, Arcioni S, Artiguenave F, Aury J-M, Ballario P, Bolchi A, Brenna A, Brun A, Buee M, Cantarel B, Chevalier G, Couloux A, Da Silva C, Denoeud F, Duplessis S, Ghignone S, Hilselberger B, Iotti M, Marcais B, Mello A, Miranda M, Pacioni G, Quesneville H, Riccioni C, Ruotolo R, Splivallo R, Stocchi V, Tisserant E, Viscomi AR, Zambonelli A, Zampieri E, Henrissat B, Lebrun M-H, Paolocci F, Bonfante P, Ottonello S, Wincker P (2010) Perigord black truffle genome uncovers evolutionary origins and mechanisms of symbiosis. Nature 464: 1033-1038.
23. Mogge B, Loferer C, Agerer R, Hutzler P, Hartmann A (2000) Bacterial community structure and colonization patterns of Fagus sylvatica l. Ectomycorrhizospheres as determined by fluorescence in situ hybridization and confocal laser scanning microscopy. Mycorrhiza 9: 271-278.
24. Mosca E, Montecchio L, Scattolin L, Garbaye J (2007) Enzymatic activities of three ectomycorrhizal types of Quercus robur l. In relation to tree decline and thinning. Soil Biol Biochem 39: 2897-2904.
25. Niederer M, Pankow W, Wiemken A (1989) Trehalose synthesis in mycorrhiza of Norway spruce: an indicator of vitality. Eur J For Pathol 19: 14-20.
26. Perez-Moreno J, Read DJ (2000) Mobilization and transfer of nutrients from litter to tree seedlings via the vegetative mycelium of ectomycorrhizal plants. New Phytol 145: 301-309.
27. Pitarch A, Sanchez M, Nombela C, Gil C (2002) Sequential fractionation and two-dimensional gel analysis unravels the complexity of the dimorphic fungus Candida albicans cell wall proteome. Mol Cell Proteomics 1(12): 967-982.
28. Plassard C, Guérin-Laguette A, Véry A-A, Casarin V, Thibaud J-B (2002) Local measurements of nitrate and potassium fluxes along roots of maritime pine. Effects of ectomycorrhizal symbiosis. Plant Cell Environ 25: 75-84.
29. Pretzsch H, Kahn M, Grote R (1998) The mixed spruce-beech forest stands of the "Sonderforschungsbereich" "growth or parasite defence?" in the forest district Kranzberger Forst. J For Res 117: 241-257.
30. Pritsch K, Garbaye J (2011) Enzyme secretion by ECM-fungi and exploitation of mineral nutrients from soil organic matter. Ann Forest Sci 68(1). doi: 10. 1007/s13595-010-0004-8.
31. Pritsch K, Raidl S, Marksteiner E, Blaschke H, Agerer R, Schloter M, Hartmann A (2004) A rapid and highly sensitive method for measuring enzyme activities in single mycorrhizal tips using 4-methylumbelliferone labelled fluorogenic substrates in a microplate system. J Microbiol Meth 58: 233-241.
32. R Development Core Team (2005) R: a language and environment for statistical computing. R Development Core Team, Vienna.
33. Rast DM, Baumgartner D, Mayer C, Hollenstein GO (2003) Cell wall-associated enzymes in fungi. Phytochemistry 64: 339-366.
34. Richard F, Millot S, Gardes M, Selosse MA (2005) Diversity and specificity of ectomycorrhizal fungi retrieved from an old-growth Mediterranean forest dominated by Quercus ilex. New Phytol 166: 1011-1023.
35. Rineau F, Garbaye J (2009) Does forest liming impact the enzymatic profiles of ectomycorrhizal communities through specialized fungal symbionts? Mycorrhiza 19: 493-500.
36. Slayman CL (1965) Electrical properties of Neurospora crassa: effects of external cations on the intracellular potential. J Gen Physiol 49: 69-92.
37. Staddon PL, Fitter AH (2001) The differential vitality of intraradical mycorrhizal structures and its implications. Soil Biol Biochem 33: 129-132.
38. Tedersoo L, Köljalg U, Hallenberg N, Larsson K-H (2003) Fine scale distribution of ectomycorrhizal fungi and roots across substrate layers including coarse woody debris in a mixed forest. New Phytol 159: 153-165.
39. Tedersoo L, Partel K, Jairus T, Gates G, Poldmaa K, Tamm H (2009) Ascomycetes associated with ectomycorrhizas: molecular diversity and ecology with particular reference to the Helotiales. Environ Microbiol 11(12): 3166-3178.
40. Tibbett M, Sanders FE (2002) Ectomycorrhizal symbiosis can enhance plant nutrition through improved access to discrete organic nutrient patches of high resource quality. Ann Bot 89: 783-789.
41. Wallenius K, Rita H, Simpanen S, Mikkonen A, Niemi RM (2010) Sample storage for soil enzyme activity and bacterial community profiles. J Microbiol Meth 81: 48-55.