High-magnification observation of seminiferous tubules through the tunica albuginea by two-photon laser scanning microscopy

Asian Journal of Andrology

Volumn 13, Issue 5, 2011, Pages 774-776

  Achard, Vincent ^a,b   Weber, Pascal ^c   Mercier, Georges ^a   Blanchard, Marie Pierre ^a  

^a AIX MARSEILLE UNIVERSITY   (France)

^b ASSISTANCE PUBLIQUE HÔPITAUX DE MARSEILLE   (France)

^c AIX MARSEILLE UNIVERSITÉ   (France)

Author keywords

seminiferous epithelium; spermatogenesis

Indexed keywords

ANIMAL EXPERIMENT; ANIMAL MODEL; ANIMAL TISSUE; ARTICLE; AZOOSPERMIA; CONTROLLED STUDY; HISTOLOGY; INFERTILITY THERAPY; LASER MICROSCOPY; MALE; MALE INFERTILITY; MOUSE; NONHUMAN; SEMINIFEROUS TUBULE; SPERMATOGENESIS; TESTICULAR SPERM EXTRACTION; TESTIS; TUNICA ALBUGINEA; TWO PHOTON LASER SCANNING MICROSCOPY;

ANIMALS; INFERTILITY, MALE; MALE; MICE; MICE, INBRED C57BL; MICROSCOPY, CONFOCAL; SEMINIFEROUS TUBULES;

EID: 80052439376     PISSN: 1008682X     EISSN: 17457262     Source Type: Journal    
DOI: 10.1038/aja.2011.6     Document Type: Article

References (13)

1. Ramasamy R, Lin K, Gosden LV, Rosenwaks Z, Palermo GD et al. High serum FSH levels in men with nonobstructive azoospermia does not affect success of microdissection testicular sperm extraction. Fertil Steril 2009; 92: 590-3.
2. Ramasamy R, Yagan N, Schlegel PN. Structural and functional changes to the testis after conventional versus microdissection testicular sperm extraction. Urology 2005; 65: 1190-4.
3. Svoboda K, Yasuda R. Principles of two-photon excitation microscopy and its applications to neuroscience. Neuron 2006; 50: 823-39.
4. Zipfel WR, Williams RM, Christie R, Nikitin AY, Hyman BT et al. Live tissue intrinsic emission microscopy using multiphoton-excited native fluorescence and second harmonic generation. Proc Natl Acad Sci USA 2003; 100: 7075-80.
5. Cahalan MD, Parker I, Wei SH, Miller MJ. Two-photon tissue imaging: seeing the immune system in a fresh light. Nat Rev Immunol 2002; 2: 872-80.
6. König K, Tadir Y, Patrizio P, Berns MW, Tromberg BJ. Effects of ultraviolet exposure and near infrared laser tweezers on human spermatozoa. Hum Reprod 1996; 11: 2162-4.
7. Greenhalgh JR, Griffith TS, Wald M. The use of immunofluorescence in microdissection testicular sperm extraction. J Androl 2009; 30: 548-51.
8. Weiss L, Greep RO. Histology. New York: McGraw-Hill Publishers; 1977. p13.
9. Billinton N, Knight AW. Seeing the wood through the trees: a review of techniques for distinguishing green fluorescent protein from endogenous autofluorescence. Anal Biochem 2001; 291: 175-97.
10. Liu Z, Lin H, Ye S, Liu QY, Meng Z et al. Remarkably high activities of testicular cytochrome c in destroying reactive oxygen species and in triggering apoptosis. Proc Natl Acad Sci USA 2006; 103: 8965-70.
11. Berndtson WE, Igboeli G, Pickett BW. Relationship of absolute numbers of Sertoli cells to testicular size and spermatogenesis in young beef bulls. J Anim Sci 1987; 64: 241-6.
12. Orth JM, Gunsalus GL, Lamperti AA. Evidence from Sertoli cell-depleted rats indicates that spermatid number in adults depends on numbers of Sertoli cells produced during perinatal development. Endocrinology 1988; 122: 787-94.
13. Johnson L, Carter GK, Varner DD, Taylor TS, Blanchard TL et al. The relationship of daily sperm production with number of Sertoli cells and testicular size in adult horses: role of primitive spermatogonia. J Reprod Fertil 1994; 100: 315-21.