Molecular identification of laser-dissected gut contents from hatchery-reared larval cod, Gadus morhua: A new approach to diet analysis

Aquaculture Nutrition

Volumn 17, Issue 5, 2011, Pages 536-541

  Maloy, A P ^a,b   Culloty, S C ^a   Bolton Warberg, M ^c   Fitzgerald, R ^c   Slater, J W ^b  

^a UNIVERSITY COLLEGE CORK   (Ireland)

^b Atlantic Technological University Sligo   (Ireland)

^c NATIONAL UNIVERSITY OF IRELAND   (Ireland)

Author keywords

Cod; Diet analysis; Gadus morhua; Larvae; Laser capture microdissection

Indexed keywords

ARTEMIA; ARTEMIA SALINA; EUKARYOTA; GADUS MORHUA;

EID: 80052409839     PISSN: 13535773     EISSN: 13652095     Source Type: Journal    
DOI: 10.1111/j.1365-2095.2010.00836.x     Document Type: Article

References (22)

1. Altschul, S.F., Madden, T.L., Schaffer, A.A., Zhang, J.H., Zhang, Z., Miller, W. & Lipman, D.J. (1997) Gapped BLAST and PSI-BLAST: a new generation of protein database search programs. Nucleic Acids Res., 25, 3389-3402.
2. Blankenship, L.E. & Yayanos, A.A. (2005) Universal primers and PCR of gut contents to study marine invertebrate diets. Mol. Ecol., 14, 891-899.
3. Bonner, R.F., EmmertBuck, M., Cole, K., Pohida, T., Chuaqui, R., Goldstein, S. & Liotta, L.A. (1997) Cell sampling - laser capture microdissection: molecular analysis of tissue. Science, 278, 1481-1483.
4. Deagle, B., Eveson, J.P. & Jarman, S. (2006) Quantification of damage in DNA recovered from highly degraded samples - a case study on DNA in faeces. Front. Zool., 3, 11.
5. Deagle, B.E., Kirkwood, R. & Jarman, S.N. (2009) Analysis of Australian fur seal diet by pyrosequencing prey DNA in faeces. Mol. Ecol., 18, 2022-2038.
6. de Figueiredo, G.M., Nash, R.D.M. & Montagnes, D.J.S. (2005) The role of the generally unrecognised microprey source as food for larval fish in the Irish Sea. Mar. Biol., 148, 395-404.
7. de Figueiredo, G.M., Nash, R.D.M. & Montagnes, D.S. (2007) Do protozoa contribute significantly to the diet of larval fish in the Irish Sea? J. Mar. Biol. Assoc. UK, 87, 843-850.
8. Fukami, K., Watanabe, A., Fujita, S., Yamaoka, K. & Nishijima, T. (1999) Predation on naked protozoan microzooplankton by fish larvae. Mar. Ecol. Prog. Ser., 185, 285-291.
9. Gast, R.J., Dennett, M.R. & Caron, D.A. (2004) Characterization of protistan assemblages in the Ross Sea, Antarctica, by denaturing gradient gel electrophoresis. Appl. Environ. Microbiol., 70, 2028-2037.
10. von Herbing, I.H. & Gallager, S.M. (2000) Foraging behavior in early Atlantic cod larvae (Gadus morhua) feeding on a protozoan (Balanion sp.) and a copepod nauplius (Pseudodiaptomus sp.). Mar. Biol., 136, 591-602.
11. Kane, J. (1984) The feeding habits of co-occurring cod and haddock larvae from Georges Bank. Mar. Ecol. Prog. Ser., 16, 9-20.
12. Maloy, A.P., Culloty, S.C. & Slater, J.W. (2009) Use of PCR-DGGE to investigate the trophic ecology of marine suspension feeding bivalves. Mar. Ecol. Prog. Ser., 381, 109-118.
13. Martin, D.L., Ross, R.M., Quetin, L.B. & Murray, A.E. (2006) Molecular approach (PCR-DGGE) to diet analysis in young Antarctic krill Euphausia superba. Mar. Ecol. Prog. Ser., 319, 155-165.
14. Medlin, L., Elwood, H.J., Stickel, S. & Sogin, M.L. (1988) The characterization of enzymatically amplified eukaryotic 16S-like rRNA-coding regions. Gene, 71, 491-499.
15. Pepin, P. & Dower, J.E. (2007) Variability in the trophic position of larval fish in a coastal pelagic ecosystem based on stable isotope analysis. J. Plankton Res., 29, 727-737.
16. Pepin, P. & Penney, R.W. (1997) Patterns of prey size and taxonomic composition in larval fish: are there general size-dependent models? J. Fish Biol., 51, 84-100.
17. Rainuzzo, J.R., Reitan, K.I. & Olsen, Y. (1997) The significance of lipids at early stages of marine fish: a review. Aquaculture, 155, 103-115.
18. Riemann, L., Alfredsson, H., Hansen, M.M. et al. (2010) Qualitative assessment of the diet of European eel larvae in the Sargasso Sea resolved by DNA barcoding. Biol. Lett., 6, 819-822.
19. Suzuki, N., Hoshino, K., Murakami, K., Takeyama, H. & Chow, S. (2008) Molecular diet analysis of phyllosoma larvae of the Japanese spiny lobster Panulirus japonicus (Decapoda: Crustacea). Mar. Biotechnol., 10, 49-55.
20. Troedsson, C., Simonelli, P., Nagele, V., Nejstgaard, J.C. & Frischer, M.E. (2009) Quantification of copepod gut content by differential length amplification quantitative PCR (dla-qPCR). Mar. Biol., 156, 253-259.
21. Vestheim, H. & Jarman, S.N. (2008) Blocking primers to enhance PCR amplification of rare sequences in mixed samples - a case study on prey DNA in Antarctic krill stomachs. Front. Zool., 5, 1-11.
22. Voss, R., Dickmann, M. & Schmidt, J.O. (2009) Feeding ecology of sprat (Sprattus sprattus L.) and sardine (Sardina pilchardus W.) larvae in the German Bight, North Sea. Oceanologia, 51, 117-138.