Plant defensin AhPDF1.1 is not secreted in leaves but it accumulates in intracellular compartments

New Phytologist

Volumn 192, Issue 1, 2011, Pages 140-150

  Oomen, Ronald J F J ^a   Séveno Carpentier, Emilie ^a   Ricodeau, Nicolas ^a   Bournaud, Caroline ^a   Conéjéro, Geneviève ^a   Paris, Nadine ^a   Berthomieu, Pierre ^a   Marquès, Laurence ^a  

^a UNIV MONTPELLIER   (France)

Author keywords

AhPDF1.1; Green fluorescent protein (GFP) fusion protein; Immunolocalization; Intracellular retention; Plant defensin; Secretion; Subcellular localization

Indexed keywords

ARABIDOPSIS PROTEIN; CHITOSAN; DEFENSIN; GREEN FLUORESCENT PROTEIN; HYBRID PROTEIN; SIGNAL PEPTIDE; ZINC;

ACCUMULATION; DICOTYLEDON; GROWTH; IMMUNOASSAY; INHIBITION; LEAF; PLANT DEFENSE; PROTEIN; ROOT; SECRETION;

ADAPTATION; AMINO ACID SEQUENCE; ARABIDOPSIS; ARTICLE; CELL COMPARTMENTALIZATION; CELL FRACTIONATION; CELL VACUOLE; CELL WALL; CHEMISTRY; CYTOLOGY; DRUG EFFECT; IMMUNOASSAY; INTRACELLULAR SPACE; METABOLISM; MOLECULAR GENETICS; PHYSIOLOGY; PLANT LEAF; PROTEIN TRANSPORT; SACCHAROMYCES CEREVISIAE; STRUCTURE ACTIVITY RELATION; TOBACCO; TRANS GOLGI NETWORK;

ADAPTATION, PHYSIOLOGICAL; AMINO ACID SEQUENCE; ARABIDOPSIS; ARABIDOPSIS PROTEINS; CELL COMPARTMENTATION; CELL WALL; CHITOSAN; DEFENSINS; GREEN FLUORESCENT PROTEINS; IMMUNOASSAY; INTRACELLULAR SPACE; MOLECULAR SEQUENCE DATA; PLANT LEAVES; PROTEIN SORTING SIGNALS; PROTEIN TRANSPORT; RECOMBINANT FUSION PROTEINS; SACCHAROMYCES CEREVISIAE; STRUCTURE-ACTIVITY RELATIONSHIP; SUBCELLULAR FRACTIONS; TOBACCO; TRANS-GOLGI NETWORK; VACUOLES; ZINC;

ARABIDOPSIS; ARABIDOPSIS HALLERI; ARABIDOPSIS THALIANA;

EID: 80052375180     PISSN: 0028646X     EISSN: 14698137     Source Type: Journal    
DOI: 10.1111/j.1469-8137.2011.03792.x     Document Type: Article

References (44)

1. Allen A, Snyder AK, Preuss M, Nielsen EE, Shah DM, Smith TJ. 2008. Plant defensins and virally encoded fungal toxin KP4 inhibit plant root growth. Planta 227: 331-339.
2. Amborabe BE, Bonmort J, Fleurat-Lessard P, Roblin G. 2008. Early events induced by chitosan on plant cells. Journal of Experimental Botany 59: 2317-2324.
3. Batoko H, Zheng HQ, Hawes C, Moore I. 2000. A Rab1 GTPase is required for transport between the endoplasmic reticulum and Golgi apparatus and for normal Golgi movement in plants. Plant Cell 12: 2201-2218.
4. de Beer A, Vivier MA. 2008. Vv-AMP1, a ripening induced peptide from Vitis vinifera shows strong antifungal activity. BMC Plant Biology 8: 75.
5. Bendtsen JD, Nielsen H, von Heijne G, Brunak S. 2004. Improved prediction of signal peptides: SignalP 3.0. Journal of Molecular Biology 340: 783-795.
6. Bert V, Macnair M, De Laguerie P, Saumitou-Laprade P, Petit D. 2000. Zinc tolerance and accumulation in metallicolous and non-metallicolous populations of Arabidopsis halleri. New Phytologist 146: 225-233.
7. Boevink P, Oparka K, Santa Cruz S, Martin B, Betteridge A, Hawes C. 1998. Stacks on tracks: the plant Golgi apparatus traffics on an actin/ER network. Plant Journal 15: 441-447.
8. Brandizzi F, Snapp EL, Roberts AG, Lippincott-Schwartz J, Hawes C. 2002. Membrane protein transport between the endoplasmic reticulum and the Golgi in tobacco leaves is energy dependent but cytoskeleton independent: evidence from selective photobleaching. Plant Cell 14: 1293-1309.
9. Carter C, Pan S, Zouhar J, Avila EL, Girke T, Raikhel NV. 2004. The vegetative vacuole proteome of Arabidopsis thaliana reveals predicted and unexpected proteins. Plant Cell 16: 3285-3303.
10. Carvalho AO, Gomes VM. 2009. Plant defensins - prospects for the biological functions and biotechnological properties. Peptides 30: 1007-1020.
11. Chatre L, Brandizzi F, Hocquellet A, Hawes C, Moreau P. 2005. Sec22 and Memb11 are v-SNAREs of the anterograde endoplasmic reticulum-Golgi pathway in tobacco leaf epidermal cells. Plant Physiology 139: 1244-1254.
12. Clough SJ. 2005. Floral dip: Agrobacterium-mediated germ line transformation. Methods in Molecular Biology 286: 91-102.
13. de Coninck BMA, Sels J, Venmans E, Thys W, Goderis IJWM, Carron D, Delauré SL, Cammue BPA, De Bolle MFC, Mathys J. 2010. Arabidopsis thaliana plant defensin AtPDF1.1 is involved in the plant response to biotic stress. New Phytologist 187: 1075-1088.
14. Cormack BP, Valdivia RH, Falkow S. 1996. FACS-optimized mutants of the green fluorescent protein (GFP). Gene 173: 33-38.
15. Dixon DC, Cutt JR, Klessig DF. 1991. Differential targeting of the tobacco PR-1 pathogenesis-related proteins to the extracellular space and vacuoles of crystal idioblasts. EMBO Journal 10: 1317-1324.
16. Ganz T, Liu L, Valore EV, Oren A. 1993. Posttranslational processing and targeting of transgenic human defensin in murine granulocyte, macrophage, fibroblast, and pituitary adenoma cell lines. Blood 82: 641-650.
17. Gao AG, Hakimi SM, Mittanck CA, Wu Y, Woerner BM, Stark DM, Shah DM, Liang J, Rommens CM. 2000. Fungal pathogen protection in potato by expression of a plant defensin peptide. Nature Biotechnology 18: 1307-1310.
18. Hamel F, Boivin R, Tremblay C, Bellemare G. 1997. Structural and evolutionary relationships among chitinases of flowering plants. Journal of Molecular Evolution 44: 614-624.
19. Hanton SL, Brandizzi F. 2006. Fluorescent proteins as markers in the plant secretory pathway. Microscopy Research and Technique 69: 152-159.
20. Horton P, Park KJ, Obayashi T, Fujita N, Harada H, Adams-Collier CJ, Nakai K. 2007. WoLF PSORT: protein localization predictor. Nucleic Acids Research 35: W585-W587.
21. Jha S, Chattoo BB. 2009. Expression of a plant defensin in rice confers resistance to fungal phytopathogens. Transgenic Research 18: 59-69.
22. Koike M, Okamoto T, Tsuda S, Imai R. 2002. A novel plant defensin-like gene of winter wheat is specifically induced during cold acclimation. Biochemical and Biophysical Research Communications 298: 46-53.
23. Kotzer AM, Brandizzi F, Neumann U, Paris N, Moore I, Hawes C. 2004. AtRabF2b (Ara7) acts on the vacuolar trafficking pathway in tobacco leaf epidermal cells. Journal of Cell Science 117: 6377-6389.
24. Kragh KM, Nielsen JE, Nielsen KK, Dreboldt S, Mikkelsen JD. 1995. Characterization and localization of new antifungal cysteine-rich proteins from Beta vulgaris. Molecular Plant-Microbe Interactions 8: 424-434.
25. Lay FT, Brugliera F, Anderson MA. 2003. Isolation and properties of floral defensins from ornamental tobacco and petunia. Plant Physiology 131: 1283-1293.
26. Li X, Gasic K, Cammue B, Broekaert W, Korban SS. 2003. Transgenic rose lines harboring an antimicrobial protein gene, Ace-AMP1, demonstrate enhanced resistance to powdery mildew (Sphaerotheca pannosa). Planta 218: 226-232.
27. Li YB, Rogers SW, Tse YC, Lo SW, Sun SS, Jauh GY, Jiang L. 2002. BP-80 and homologs are concentrated on post-Golgi, probable lytic prevacuolar compartments. Plant and Cell Physiology 43: 726-742.
28. Marquès L, Oomen RJ, Aumelas A, Le Jean M, Berthomieu P. 2009. Production of an Arabidopsis halleri foliar defensin in Escherichia coli. Journal of Applied Microbiology 106: 1640-1648.
29. Mirouze M, Sels J, Richard O, Czernic P, Loubet S, Jacquier A, Francois IE, Cammue BP, Lebrun M, Berthomieu P et al. 2006. A putative novel role for plant defensins: a defensin from the zinc hyper-accumulating plant, Arabidopsis halleri, confers zinc tolerance. Plant Journal 47: 329-342.
30. Mitta G, Vandenbulcke F, Hubert F, Roch P. 1999. Mussel defensins are synthesised and processed in granulocytes then released into the plasma after bacterial challenge. Journal of Cell Science 112: 4233-4242.
31. Neuhaus JM, Sticher L, Meins F Jr, Boller T. 1991. A short C-terminal sequence is necessary and sufficient for the targeting of chitinases to the plant vacuole. Proceedings of the National Academy of Sciences, USA 88: 10362-10366.
32. Osborn RW, De Samblanx GW, Thevissen K, Goderis I, Torrekens S, Van Leuven F, Attenborough S, Rees SB, Broekaert WF. 1995. Isolation and characterisation of plant defensins from seeds of Asteraceae, Fabaceae, Hippocastanaceae and Saxifragaceae. FEBS Letters 368: 257-262.
33. Paris N, Neuhaus JM. 2002. BP-80 as a vacuolar sorting receptor. Plant Molecular Biology 50: 903-914.
34. Paris N, Rogers SW, Jiang L, Kirsch T, Beevers L, Phillips TE, Rogers JC. 1997. Molecular cloning and further characterization of a probable plant vacuolar sorting receptor. Plant Physiology 115: 29-39.
35. Sambrook J, Fritsch EF, Maniatis T. 1989. Molecular cloning: a laboratory manual. Cold Spring Harbor, NY, USA: Cold Spring Harbor Laboratory Press.
36. di Sansebastiano GP, Paris N, Marc-Martin S, Neuhaus JM. 1998. Specific accumulation of GFP in a non-acidic vacuolar compartment via a C-terminal propeptide-mediated sorting pathway. Plant Journal 15: 449-457.
37. di Sansebastiano GP, Paris N, Marc-Martin S, Neuhaus JM. 2001. Regeneration of a lytic central vacuole and of neutral peripheral vacuoles can be visualized by green fluorescent proteins targeted to either type of vacuoles. Plant Physiology 126: 78-86.
38. Sticher L, Hofsteenge J, Milani A, Neuhaus JM, Meins F Jr. 1992. Vacuolar chitinases of tobacco: a new class of hydroxyproline-containing proteins. Science 257: 655-657.
39. Tamaoki M, Freeman J, Marques L, Pilon-Smits E. 2008. New insights into the roles of ethylene and jasmonic acid in the acquisition of selenium resistance in plants. Plant Signaling & Behavior 3: 865-867.
40. Tavares LS, Santos Mde O, Viccini LF, Moreira JS, Miller RN, Franco OL. 2008. Biotechnological potential of antimicrobial peptides from flowers. Peptides 29: 1842-1851.
41. Terras FR, Eggermont K, Kovaleva V, Raikhel NV, Osborn RW, Kester A, Rees SB, Torrekens S, Van Leuven F, Vanderleyden J et al. 1995. Small cysteine-rich antifungal proteins from radish: their role in host defense. Plant Cell 7: 573-588.
42. Thomma BP, Cammue BP, Thevissen K. 2002. Plant defensins. Planta 216: 193-202.
43. Turrini A, Sbrana C, Pitto L, Ruffini Castiglione M, Giorgetti L, Briganti R, Bracci T, Evangelista M, Nuti MP, Giovannetti M. 2004. The antifungal Dm-AMP1 protein from Dahlia merckii expressed in Solanum melongena is released in root exudates and differentially affects pathogenic fungi and mycorrhizal symbiosis. New Phytologist 163: 393-403.
44. de Zélicourt A, Letousey P, Thoiron S, Campion C, Simoneau P, Elmorjani K, Marion D, Simier P, Delavault P. 2007. Ha-DEF1, a sunflower defensin, induces cell death in Orobanche parasitic plants. Planta 226: 591-600.