Chemical de-O-glycosylation of glycoproteins for applications in LC-based proteomics

Methods in Molecular Biology

Volumn 753, Issue , 2011, Pages 323-333

  Hanisch, Franz Georg ^a  

^a UNIVERSITY OF COLOGNE   (Germany)

Author keywords

Chemical deglycosylation; Liquid chromatographymass spectrometry; O glycoproteins; O glycoproteome; Proteomics

Indexed keywords

GLYCOPEPTIDE; GLYCOPROTEIN; POLYSACCHARIDE;

AMINO ACID SEQUENCE; ANIMAL; ARTICLE; CHEMICAL STRUCTURE; CHEMISTRY; ELECTROSPRAY MASS SPECTROMETRY; GENETICS; GLYCOSYLATION; HUMAN; INSTRUMENTATION; LIQUID CHROMATOGRAPHY; METABOLISM; METHODOLOGY; MOLECULAR GENETICS; PROTEOMICS;

AMINO ACID SEQUENCE; ANIMALS; CHROMATOGRAPHY, LIQUID; GLYCOPEPTIDES; GLYCOPROTEINS; GLYCOSYLATION; HUMANS; MOLECULAR SEQUENCE DATA; MOLECULAR STRUCTURE; POLYSACCHARIDES; PROTEOMICS; SPECTROMETRY, MASS, ELECTROSPRAY IONIZATION;

EID: 80052363560     PISSN: 10643745     EISSN: None     Source Type: Book Series    
DOI: 10.1007/978-1-61779-148-2_22     Document Type: Article

References (11)

1. Ishii-Karakasa, I., Iwase, H., Hotta, K. (1997) Structural determination of the O-linked sialyl oligosaccharides liberated from fetuin with endo-α-N-acetylgalactosaminidase-S by HPLC analysis and 600-MHz 1H-NMR spectroscopy. Eur. J. Biochem. 247, 709-715
2. Umemoto, J., Bhavanandan, V.P., Davidson, E.A. (1977) Purification and properties of an endo-α-N-acetyl-D-galactosaminidase from Diplococcus pneumoniae. J. Biol. Chem. 252, 8609-8614
3. Patel, T.P., Parekh, R.B. (1994) Release of oligosaccharides from glycoproteins by hydrazinolysis. Methods Enzymol. 230, 57-66
4. Sojar, H.T., Bahl, O.M.P. (1987) Chemical deglycosylation of glycoproteins. Arch. Biochem. Biophys. 259, 52-57
5. Gerken, T.A., Gupta, R., Jentoft, N. (1992) A novel approach for chemically deglycosylating O-linked glycoproteins. The deglycosylation of submaxillary and respiratory mucins. Biochemistry 31, 639-648
6. Rademaker, G.J., Pergantis, S.A., Blok-Tip, L.A., Langridge, J.I., et al. (1998) Mass spectrometric determination of the sites of O-glycan attachment with low picomolar sensitivity. Anal. Biochem. 257, 149-160
7. Hanisch, F.-G., Jovanovic, M., Peter- Katalinic, J. (2001) Glycoprotein identification and localization of O-glycosylation sites by mass spectrometric analysis of deglycosylated/alkylaminylated peptide fragments. Anal. Biochem. 290, 47-59
8. Hong, J.C., Kim, Y.S. (2000) Alkalicatalyzed beta-elimination of periodateoxidized glycans: a novel method of chemical deglycosylation of mucin gene products in paraffin-embedded sections. Glycoconj. J. 17, 691-703
9. Hanisch, F.-G., Teitz, S., Schwientek, T., Müller, S. (2009) Chemical de-Oglycosylation of glycoproteins for application in LC-based proteomics. Proteomics 9, 710-719
10. Müller, S., Hanisch, F.G. (2002) Recombinant MUC1 probe authentically reflects cellspecific O-glycosylation profiles of endogenous breast cancer mucin. J. Biol. Chem. 277, 26103-26112
11. Engelmann, K., Kinlough, C.L., Müller, S., Razawi, H., et al. (2005) Transmembrane and secreted MUC1 probes show traffickingdependent changes in O-glycan core profiles. Glycobiology 15, 1111-1124