Exploration of overloaded cation exchange chromatography for monoclonal antibody purification

Journal of Chromatography A

Volumn 1218, Issue 39, 2011, Pages 6943-6952

  Liu, Hui F ^a   McCooey, Beth ^a   Duarte, Tiago ^a   Myers, Deanna E ^a   Hudson, Terry ^a   Amanullah, Ashraf ^a   van Reis, Robert ^a   Kelley, Brian D ^a  

^a GENENTECH INC   (United States)

Author keywords

Cation exchange; Chromatography; Conventional and convective chromatography matrices; Dynamic binding capacity; High molecular weight species; Monoclonal antibody purification; Overloaded chromatography

Indexed keywords

CATION EXCHANGES; CONVENTIONAL AND CONVECTIVE CHROMATOGRAPHY MATRICES; DYNAMIC BINDING CAPACITY; HIGH MOLECULAR WEIGHT; MONOCLONAL ANTIBODY PURIFICATION; OVERLOADED CHROMATOGRAPHY;

AFFINITY CHROMATOGRAPHY; CELL CULTURE; GEL PERMEATION CHROMATOGRAPHY; ION EXCHANGE RESINS; LEACHING; MOLECULAR WEIGHT; MONOCLONAL ANTIBODIES; MONOLITHIC INTEGRATED CIRCUITS; PROTEINS; PURIFICATION; SURFACE PLASMON RESONANCE;

POSITIVE IONS;

CATION EXCHANGE RESIN; CELL PROTEIN; GENTAMICIN; MONOCLONAL ANTIBODY; PROTEIN A; SEPHAROSE;

AFFINITY CHROMATOGRAPHY; ANTIBODY PRODUCTION; ANTIGEN BINDING; ARTICLE; BINDING AFFINITY; CHEMICAL ANALYSIS; CONDUCTANCE; CONTROLLED STUDY; DRUG IMPURITY; FLOW RATE; HOST CELL; IMMUNOGLOBULIN PURIFICATION; ION EXCHANGE CHROMATOGRAPHY; MOLECULAR WEIGHT; PHYSICAL PHASE; PRIORITY JOURNAL; PROCESS OPTIMIZATION; PURIFICATION;

ANIMALS; ANTIBODIES, MONOCLONAL; CATION EXCHANGE RESINS; CHO CELLS; CHROMATOGRAPHY, AFFINITY; CHROMATOGRAPHY, ION EXCHANGE; CRICETINAE; CRICETULUS; DNA; HUMANS; IMMUNOGLOBULIN G; MEMBRANES, ARTIFICIAL; MOLECULAR WEIGHT; PROTEIN BINDING; PROTEINS; STAPHYLOCOCCAL PROTEIN A;

EID: 80052341151     PISSN: 00219673     EISSN: 18733778     Source Type: Journal    
DOI: 10.1016/j.chroma.2011.08.008     Document Type: Article

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