Single-microchannel, multistep assay reports protein size and immunoaffinity

Analytical Chemistry

Volumn 83, Issue 17, 2011, Pages 6573-6579

  Chen, Xiaofang ^a   Kapil, Monica A ^a   Hughes, Alex J ^a   Herr, Amy E ^a  

^a University of California at Berkeley   (United States)

Author keywords

[No Author keywords available]

Indexed keywords

CELL SIGNALING; CHEMICAL FUNCTIONALIZATION; DESIGN STRATEGIES; DETECTION LIMITS; END USERS; FULLY INTEGRATED; HETEROGENEOUS IMMUNOASSAY; IMMOBILIZED ANTIBODIES; IMMUNOAFFINITY; MIGRATION DISTANCE; MULTI-STEP; PEAK CAPACITY; PROOF OF PRINCIPLES; PROTEIN ASSAYS; PROTEIN IDENTITY; PROTEIN SIZE; PROTEIN SPECIES; SEPARATION DISTANCES; SIEVING MATRIX; SINGLE MICROCHANNEL; SINGLE-CHANNEL;

ANTIGEN-ANTIBODY REACTIONS; CELL PROLIFERATION; ELECTROPHORESIS; GLYCOPROTEINS; MAMMALS; MICROCHANNELS; MOLECULAR WEIGHT;

ANTIBODIES;

FOLLISTATIN; IMMOBILIZED ANTIBODY;

ARTICLE; CHEMISTRY; IMMUNOASSAY; IMMUNOLOGY; METHODOLOGY; MICROFLUIDIC ANALYSIS; POLYACRYLAMIDE GEL ELECTROPHORESIS;

ANTIBODIES, IMMOBILIZED; ELECTROPHORESIS, POLYACRYLAMIDE GEL; FOLLISTATIN; IMMUNOASSAY; MICROFLUIDIC ANALYTICAL TECHNIQUES;

EID: 80052336132     PISSN: 00032700     EISSN: None     Source Type: Journal    
DOI: 10.1021/ac200982j     Document Type: Article

References (28)

1. Towbin, H.; Staehelin, T.; Gordon, J. Proc. Natl. Acad. Sci. U.S.A. 1979, 76, 4350-4354
2. Burnette, W. N. Anal. Biochem. 1981, 112, 195-203
3. Wu, Y.; Li, Q.; Chen, X. Z. Nat. Protoc. 2007, 2, 3278-3284
4. Hou, C.; Herr, A. E. Electrophoresis 2008, 29, 3306-3319
5. Johansen, K.; Svensson, L. In The Protein Protocols Handbook, 2 nd ed.; Walker, J. M., Ed.; Humana Press, Inc.: Totowa, NJ, 2002; p 1097.
6. Chen, H. X.; Busnel, J. M.; Peltre, G.; Zhang, X. X.; Girault, H. H. Anal. Chem. 2008, 80, 9583-9588
7. Phillips, T. M.; Wellner, E. F. Electrophoresis 2007, 28, 3041-3048
8. Chun, H.; Chung, T. D.; Ramsey, J. M. Anal. Chem. 2010, 82, 6287-6292
9. Yang, W.; Yu, M.; Sun, X.; Woolley, A. T. Lab Chip 2010, 10, 2527-2533
10. Phillips, T. M.; Wellner, E. F. Biomed. Chromatogr. 2006, 20, 662-667
11. Phillips, T. M.; Wellner, E. F. Electrophoresis 2009, 30, 2307-2312
12. Hatch, A. V.; Herr, A. E.; Throckmorton, D. J.; Brennan, J. S.; Singh, A. K. Anal. Chem. 2006, 78, 4976-4984
13. Apori, A. A.; Herr, A. E. Anal. Chem. 2011, 83
14. Herr, A. E.; Hatch, A. V.; Throckmorton, D. J.; Tran, H. M.; Brennan, J. S.; Giannobile, W. V.; Singh, A. K. Proc. Natl. Acad. Sci. U.S.A. 2007, 104, 5268-5273
15. Hughes, A. J.; Herr, A. E. Anal. Chem. 2010, 82, 3803-3811
16. Sommer, G. J.; Singh, A. K.; Hatch, A. V. Lab Chip 2009, 9, 2729-2737
17. Margolis, J.; Kenrick, K. C. Nature 1967, 214, 1334-1336
18. Slater, G. G. Anal. Chem. 1969, 41, 1039-1041
19. Margolis, J.; Kenrick, K. G. Anal. Biochem. 1968, 25, 347-362
20. Tiselius, A. Trans. Faraday Soc. 1937, 33, 524
21. Østergaard, J.; Heegaard, N. H. H. Electrophoresis 2003, 24, 2903-2913
22. Shackman, J. G.; Munson, M. S.; Ross, D. Anal. Chem. 2007, 79, 565-571
23. Escobedo, C.; Brolo, A. G.; Gordon, R.; Sinton, D. Anal. Chem. 2010, 82, 10015-10020
24. Bharadwaj, R.; Park, C. C.; Kazakova, I.; Xu, H.; Paschkewitz, J. S. Anal. Chem. 2008, 80, 129-134
25. Squires, T. M.; Messinger, R. J.; Manalis, S. R. Nat. Biotechnol. 2008, 26, 417-426
26. Urbanek, M.; Wu, X.; Vickery, K. R.; Kao, L. C.; Christenson, L. K.; Schneyer, A.; Legro, R. S.; Driscoll, D. A.; Strauss, J. F., 3rd; Dunaif, A.; Spielman, R. S. J. Clin. Endocrinol. Metab. 2000, 85, 4455-4461
27. Tortoriello, D. V.; Sidis, Y.; Holtzman, D. A.; Holmes, W. E.; Schneyer, A. L. Endocrinology 2001, 142, 3426-3434
28. Selby, C. Ann. Clin. Biochem. 1999, 36, 704-721