Methods to Monitor Cell Surface Expression and Endocytic Trafficking of CFTR in Polarized Epithelial Cells

Methods in Molecular Biology

Volumn 741, Issue , 2011, Pages 271-283

  Bomberger, Jennifer M ^a   Guggino, William B ^b   Stanton, Bruce A ^a  

^a DARTMOUTH MEDICAL SCHOOL   (United States)

^b JOHNS HOPKINS UNIVERSITY SCHOOL OF MEDICINE   (United States)

Author keywords

Cystic fibrosis; cystic fibrosis transmembrane conductance regulator; endocytosis; intracellular trafficking; recycling

Indexed keywords

TRANSMEMBRANE CONDUCTANCE REGULATOR;

ARTICLE; BIOTINYLATION; CELL POLARITY; CYTOLOGY; ENDOCYTOSIS; EPITHELIUM CELL; GENE EXPRESSION REGULATION; HUMAN; INTRACELLULAR SPACE; METABOLISM; METHODOLOGY;

BIOTINYLATION; CELL POLARITY; CYSTIC FIBROSIS TRANSMEMBRANE CONDUCTANCE REGULATOR; CYTOLOGICAL TECHNIQUES; ENDOCYTOSIS; EPITHELIAL CELLS; GENE EXPRESSION REGULATION; HUMANS; INTRACELLULAR SPACE;

EID: 80052313356     PISSN: 10643745     EISSN: 19406029     Source Type: Book Series    
DOI: 10.1007/978-1-61779-117-8_18     Document Type: Chapter

References (6)

1. Guggino, W. B., and Stanton, B. A. (2006) New insights into cystic fibrosis: Molecular switches that regulate CFTR. Nat. Rev. Mol. Cell. Biol. 7, 426–436.
2. Gruenberg, J., and van der Goot, F. G. (2006) Mechanisms of pathogen entry through the endosomal compartments. Nat. Rev. Mol. Cell. Biol. 7, 495–504.
3. Swiatecka-Urban, A., Boyd, C., Coutermarsh, B., Karlson, K. H., Barnaby, R., Aschenbrenner, L., et al. (2004) Myosin VI regulates endocytosis of the cystic fibrosis transmembrane conductance regulator. J. Biol. Chem. 279, 38025–38031.
4. Bomberger, J. M., Barnaby, R. L., and Stanton, B. A. (2009) The deubiquitinating enzyme USP10 regulates the post-endocytic sorting of cystic fibrosis transmembrane conductance regulator in airway epithelial cells. J. Biol. Chem. 284, 18778–18789.
5. Swiatecka-Urban, A., Talebian, L., Kanno, E., Moreau-Marquis, S., Coutermarsh, B., Hansen, K., et al. (2007) Myosin VB is required for trafficking of CFTR in RAB11A-specific apical recycling endosomes in polarized human airway epithelial cells. J. Biol. Chem. 282, 23725–23736.
6. Biemesderfer, D., Mentone, S. A., Mooseker, M., and Hasson, T. (2002) Expression of myosin VI within the early endocytic pathway in adult and developing proximal tubules. Am. J. Physiol. Renal Physiol. 282, F785– F794.