Frozen tissue can provide reproducible proteomic results of subcellular fractionation

Analytical Biochemistry

Volumn 418, Issue 1, 2011, Pages 78-84

  Lim, Jihyeon ^a   Menon, Vilas ^a   Bitzer, Markus ^b   Miller, Leah M ^a   Madrid Aliste, Carlos ^a   Weiss, Louis M ^a   Fiser, Andras ^a   Angeletti, Ruth H ^a  

^a ALBERT EINSTEIN COLLEGE OF MEDICINE   (United States)

^b UNIVERSITY OF MICHIGAN   (United States)

Author keywords

Differential detergent fractionation; Frozen tissue; Normalized spectral index; Subcellular location

Indexed keywords

CELL PROLIFERATION; CYTOLOGY; HISTOLOGY; LIQUID CHROMATOGRAPHY; MASS SPECTROMETRY; PROTEINS;

CELLULAR COMPARTMENTS; CORRELATION COEFFICIENT; FROZEN TISSUES; LIQUID CHROMATOGRAPHY TANDEM MASS SPECTROMETRY (LC MS/MS); SPECTRAL INDICES; SUBCELLULAR COMPARTMENTS; SUBCELLULAR FRACTIONATION; SUBCELLULAR LOCATION;

TISSUE;

BUFFER; CELL MEMBRANE PROTEIN; CYTOPLASM PROTEIN; DETERGENT; MEMBRANE PROTEIN; NUCLEAR PROTEIN;

ANIMAL CELL; ANIMAL TISSUE; ARTICLE; CELL FRACTIONATION; CONTROLLED STUDY; CORRELATION COEFFICIENT; DIFFERENTIAL DETERGENT FRACTIONATION; FROZEN SECTION; INTRACELLULAR SPACE; LIQUID CHROMATOGRAPHY; MOUSE; NONHUMAN; PRIORITY JOURNAL; PROTEIN ANALYSIS; PROTEOMICS; REPRODUCIBILITY; TANDEM MASS SPECTROMETRY;

EID: 80052270290     PISSN: 00032697     EISSN: 10960309     Source Type: Journal    
DOI: 10.1016/j.ab.2011.06.045     Document Type: Article

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