Adaptation of ubiquitin-PNA based sperm quality assay for semen evaluation by a conventional flow cytometer and a dedicated platform for flow cytometric semen analysis

Theriogenology

Volumn 76, Issue 6, 2011, Pages 1168-1176

  Odhiambo, J F ^a,d   Sutovsky, M ^a   DeJarnette, J M ^c   Marshall, C ^c   Sutovsky, P ^a,b  

^a UNIVERSITY OF MISSOURI   (United States)

^b University of Missouri   (United States)

^c Select Sires Inc   (United States)

^d UNIVERSITY OF ALBERTA   (Canada)

Author keywords

Flow cytometry; Lectin PNA; Semen evaluation; Sperm; Ubiquitin

Indexed keywords

BIOLOGICAL MARKER; LECTIN; UBIQUITIN;

ACROSOME; ANIMAL; ANIMAL DISEASE; ARTICLE; CHEMISTRY; CYTOLOGY; FLOW CYTOMETRY; FLUORESCENCE; MALE; METABOLISM; METHODOLOGY; PATHOLOGY; SEMEN ANALYSIS; SPERMATOZOON;

ACROSOME; ANIMALS; BIOLOGICAL MARKERS; FLOW CYTOMETRY; FLUORESCENCE; LECTINS; MALE; SEMEN ANALYSIS; SPERMATOZOA; UBIQUITIN;

ARACHIS HYPOGAEA;

EID: 80052259305     PISSN: 0093691X     EISSN: None     Source Type: Journal    
DOI: 10.1016/j.theriogenology.2011.05.009     Document Type: Article

References (21)

1. Gillan L., Evans G., Maxwell W.M. Flow cytometric evaluation of sperm parameters in relation to fertility potential. Theriogenology 2005, 63:445-457.
2. DeJarnette J.M., Amann R.P. Understanding estimates of AI sire fertility: From A to Z 2010, 23rd Technical Conference on Artificial Insemination & Reproduction, Milwaukee, WI.
3. Graham J.K. Assessment of sperm quality: a flow cytometric approach. Anim Reprod Sci 2001, 68:239-247.
4. Garner D.L. Ancillary tests of bull semen quality. Vet Clin North Am Food Anim Pract 1997, 13:313-330.
5. Nagy S., Jansen J., Topper E.K., Gadella B.M. A triple-stain flow cytometric method to assess plasma- and acrosome-membrane integrity of cryopreserved bovine sperm immediately after thawing in presence of egg-yolk particles. Biol Reprod 2003, 68:1828-1835.
6. Lovercamp K.W., Safranski T.J., Fischer K.A., Manandhar G., Sutovsky M., Herring W., et al. Arachidonate 15-lipoxygenase and ubiquitin as fertility markers in boars. Theriogenology 2007, 67:704-718.
7. Sutovsky P., Terada Y., Schatten G. Ubiquitin-based sperm assay for the diagnosis of male factor infertility. Hum Reprod 2001, 16:250-258.
8. Sutovsky P., Neuber E., Schatten G. Ubiquitin-dependent sperm quality control mechanism recognizes spermatozoa with DNA defects as revealed by dual ubiquitin-TUNEL assay. Mol Reprod Dev 2002, 61:406-413.
9. Sutovsky P., Plummer W., Baska K., Peterman K., Diehl J.R., Sutovsky M. Relative levels of semen platelet activating factor-receptor (PAFr) and ubiquitin in yearling bulls with high content of semen white blood cells: implications for breeding soundness evaluation. J Androl 2007, 28:92-108.
10. Sutovsky P., Turner R.M., Hameed S., Sutovsky M. Differential ubiquitination of stallion sperm proteins: possible implications for infertility and reproductive seasonality. Biol Reprod 2003, 68:688-698.
11. Baska K.M., Manandhar G., Feng D., Agca Y., Tengowski M.W., Sutovsky M., et al. Mechanism of extracellular ubiquitination in the mammalian epididymis. J Cell Physiol 2008, 215:684-696.
12. Sutovsky P., Moreno R., Ramalho-Santos J., Dominko T., Thompson W.E., Schatten G. A putative, ubiquitin-dependent mechanism for the recognition and elimination of defective spermatozoa in the mammalian epididymis. J Cell Sci 2001, 114:1665-1675.
13. Rawe V.Y., Olmedo S.B., Benmusa A., Shiigi S.M., Chemes H.E., Sutovsky P. Sperm ubiquitination in patients with dysplasia of the fibrous sheath. Hum Reprod 2002, 17:2119-2127.
14. Ozanon C., Chouteau J., Sutovsky P. Clinical adaptation of the sperm ubuquitin tag immunoassay (SUTI): relationship of sperm ubiquitylation with sperm quality in gradient-purified semen samples from 93 men from a general infertility clinic population. Hum Reprod 2005, 20:2271-2278.
15. Cheng F.P., Fazeli A., Voorhout W.F., Marks A., Bevers M.M., Colenbrander B. Use of peanut agglutinin to assess the acrosomal status and the zona pellucida-induced acrosome reaction in stallion spermatozoa. J Androl 1996, 17:674-682.
16. Cooper T.G., Yeung C.H. A flow cytometric technique using peanut agglutinin for evaluating acrosomal loss from human spermatozoa. J Androl 1998, 19:542-550.
17. Vogler C.J., Saacke R.G., Bame J.H., DeJarnette J.M., McGilliard M.L. Effects of scrotal insulation on viability characteristics of cryopreserved bovine semen. J Dairy Sci 1991, 74:3827-3835.
18. Cross N.L., Morales P., Overstreet J.W., Hanson F.W. Two simple methods for detecting acrosome-reacted human sperm. Gamete Res 1986, 15:213-226.
19. Mortimer D., Curtis E.F., Dravland J.E. Specific labeling by peanut agglutinin of the outer acrosomal membrane of the human spermatozoon. J Reprod Fertil 1987, 81:127-135.
20. Chan J.Z., Krause W., Bohring C. Computer-assisted analysis of sperm morphology with the aid of lectin staining. Andrologia 2002, 34:379-383.
21. Verstegen J., Iguer-Ouada M., Onclin K. Computer assisted semen analyzers in andrology research and veterinary practice. Theriogenology 2002, 57:149-179.