Evaluation of real-time reverse transcriptase PCR assays for detection of pandemic influenza A/H1N1 2009 virus

Journal of Clinical Microbiology

Volumn 49, Issue 9, 2011, Pages 3444-

  Chidlow, G R ^a   Harnett, G B ^a   Speers, D J ^a   Smith, D W ^a  

^a PATHWEST LABORATORY MEDICINE   (Australia)

Author keywords

[No Author keywords available]

Indexed keywords

INFLUENZA VIRUS A H1N1; LETTER; LIMIT OF DETECTION; MELTING POINT; MOLECULAR PROBE; NONHUMAN; POLYMERASE CHAIN REACTION; PRIORITY JOURNAL; REAL TIME POLYMERASE CHAIN REACTION; REAL TIME REVERSE TRANSCRIPTASE POLYMERASE CHAIN REACTION; SENSITIVITY AND SPECIFICITY; VIRUS DETECTION; VIRUS GENOME; VIRUS STRAIN;

DNA PRIMERS; HUMANS; INFLUENZA A VIRUS, H1N1 SUBTYPE; INFLUENZA, HUMAN; MOLECULAR DIAGNOSTIC TECHNIQUES; REVERSE TRANSCRIPTASE POLYMERASE CHAIN REACTION; VIROLOGY;

EID: 80052233313     PISSN: 00951137     EISSN: 1098660X     Source Type: Journal    
DOI: 10.1128/JCM.00974-11     Document Type: Letter

References (5)

1. Afonina, I., I. Kutyavin, E. Lukhtanov, R. B. Meyer, and H. Gamper. 1996. Sequence-specific arrest of primer extension on single-stranded DNA by an oligonucleotide-minor groove binder conjugate. Proc. Natl. Acad. Sci. U. S. A. 93:3199-3204.
2. Chidlow, G., et al. 2010. Duplex real-time reverse transcriptase PCR assays for rapid detection and identification of pandemic (H1N1) 2009 and seasonal influenza A/H1, A/H3, and B viruses. J. Clin. Microbiol. 48:862-866.
3. Kutyavin, I. V., et al. 2000. 3′-Minor groove binder-DNA probes increase sequence specificity at PCR extension temperatures. Nucleic Acids Res. 28: 655-661.
4. Wang, R., Z.-M. Sheng, and J. K. Taubenberger. 2009. Detection of novel (swine origin) H1N1 influenza A virus by quantitative real-time reverse transcription- PCR. J. Clin. Microbiol. 47:2675-2677.
5. Yang, Y., et al. 2011. Evaluation of twelve real-time reverse transcriptase PCR primer-probe sets for detection of pandemic influenza A/H1N1 2009 virus. J. Clin. Microbiol. 49:1434-1440.