The quest for an optimized protocol for whole-heart decellularization: A comparison of three popular and a novel decellularization technique and their diverse effects on crucial extracellular matrix qualities

Tissue Engineering - Part C: Methods

Volumn 17, Issue 9, 2011, Pages 915-926

  Akhyari, Payam ^a   Aubin, Hug ^b   Gwanmesia, Patricia ^b   Barth, Mareike ^c   Hoffmann, Stefanie ^b   Huelsmann, Jörn ^a   Preuss, Karlheinz ^d   Lichtenberg, Artur ^a  

^a HEINRICH HEINE UNIVERSITY   (Germany)

^b UNIVERSITY HOSPITAL HEIDELBERG   (Germany)

^c GERMAN CANCER RESEARCH CENTER DKFZ   (Germany)

^d Engineo GmbH   (Germany)

Author keywords

[No Author keywords available]

Indexed keywords

BIOCOMPATIBILITY; HEART; TISSUE;

BIOCHEMICAL LEVEL; C2C12 MYOBLASTS; CARDIAC TISSUE ENGINEERING; CORONARY PERFUSION; DECELLULARIZATION; DECELLULARIZED; DNA CONTENT; ECM COMPOSITION; EXTRACELLULAR MATRICES; GLYCOSAMINOGLYCANS; IN-VITRO; LAMININ; MICROCOMPOSITION; OPTIMIZED PROTOCOL; RAT HEART; SYNTHETIC MATERIALS;

SCAFFOLDS (BIOLOGY);

RATTUS;

DNA; GLYCOSAMINOGLYCAN; TISSUE SCAFFOLD;

ANIMAL; ARTICLE; AUTOMATION; CELL DEATH; CELL LINE; COMPARATIVE STUDY; COMPUTER PROGRAM; CULTURE TECHNIQUE; CYTOLOGY; EXTRACELLULAR MATRIX; HEART MUSCLE; IMMUNOHISTOCHEMISTRY; IN VITRO STUDY; MALE; METABOLISM; METHODOLOGY; MOUSE; RAT;

ANIMALS; AUTOMATION; CELL CULTURE TECHNIQUES; CELL DEATH; CELL LINE; DNA; EXTRACELLULAR MATRIX; GLYCOSAMINOGLYCANS; IMMUNOHISTOCHEMISTRY; MALE; MICE; MYOCARDIUM; RATS; SOFTWARE; TISSUE SCAFFOLDS;

EID: 80052214904     PISSN: 19373384     EISSN: 19373392     Source Type: Journal    
DOI: 10.1089/ten.tec.2011.0210     Document Type: Article

References (40)

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20. Jacot, J.G., Kita-Matsuo, H., Wei, K.A., Chen, H.S., Omens, J.H., Mercola, M., and McCulloch, A.D. Cardiac myocyte force development during differentiation and maturation. Ann N Y Acad Sci 11, 88121, 2010.
21. Lutolf, M.P., and Hubbell, J.A. Synthetic biomaterials as instructive extracellular microenvironments for morphogenesis in tissue engineering. Nat Biotechnol 23, 47, 2005.
22. Hubbell, J.A. Matrix-Bound growth factors in tissue repair. Swiss Med Wkly 137 Suppl, 15572S, 2007.
23. Aubin, H., Nichol, J.W., Hutson, C.B., Bae, H., Sieminski, A.L., Cropek, D.M., Akhyari, P., and Khademhosseini, A. Directed 3D cell alignment and elongation in microengineered hydrogels. Biomaterials 31, 6941, 2010.
24. Lichtenberg, A., Tudorache, I., Cebotari, S., Suprunov, M., Tudorache, G., Goerler, H., Park, J.K., Hilfiker-Kleiner, D., Ringes-Lichtenberg, S., Karck, M., Brandes, G., Hilfiker, A., and Haverich, A. Preclinical testing of tissue-engineered heart valves re-endothelialized under simulated physiological conditions. Circulation 114(1 Suppl), I559, 2006.
25. Cebotari, S., Lichtenberg, A., Tudorache, I., Hilfiker, A., Mertsching, H., Leyh, R., Breymann, T., Kallenbach, K., Maniuc, L., Batrinac, A., Repin, O., Maliga, O., Ciubotaru, A., and Haverich, A. Clinical application of tissue engineered human heart valves using autologous progenitor cells. Circulation 114(1 Suppl), I132, 2006.
26. Badylak, S.F., Freytes, D.O., and Gilbert, T.W. Extracellular matrix as a biological scaffold material: Structure and function. Acta Biomater 5, 1, 2009.
27. Akhyari, P., Kamiya, H., Gwanmesia, P., Aubin, H., Tschierschke, R., Hoffmann, S., Karck, M., and Lichtenberg, A. In vivo functional performance and structural maturation of decellularised allogenic aortic valves in the subcoronary position. Eur J Cardiothorac Surg 38, 539, 2010.
28. Ott, H.C., Matthiesen, T.S., Goh, S.K., Black, L.D., Kren, S.M., Netoff, T.I., and Taylor, D.A. Perfusion-decellularized matrix: using nature's platform to engineer a bioartificial heart. Nat Med 14, 213, 2008.
29. Wainwright, J.M., Czajka, C.A., Patel, U.B., Freytes, D.O., Tobita, K., Gilbert, T.W., and Badylak, S.F. Preparation of cardiac extracellular matrix from an intact porcine heart. Tissue Eng Part C Methods 16, 525, 2010.
30. Haase, S.C., Rovak, J.M., Dennis, R.G., Kuzon, W.M., and Cederna, P.S. Recovery of muscle contractile function following nerve gap repair with chemically acellularized peripheral nerve grafts. J Reconstr Microsurg 19, 241, 2003.
31. Borschel, G.H., Dennis, R.G., and Kuzon, W.M. Contractile skeletal muscle tissue-engineered on an acellular scaffold. Plast Reconstr Surg 113, 595, 2004; discussion 603.
32. Gilbert, T.W., Sellaro, T.L., and Badylak, S.F. Decellularization of tissues and organs. Biomaterials 27, 3675, 2006.
33. Seddon, A.M., Curnow, P., and Booth, P.J. Membrane proteins, lipids and detergents: Not just a soap opera. Biochim Biophys Acta 1666, 105, 2004.
34. Joseph, J., Joseph, L., Shekhawat, N.S., Devi, S., Wang, J., Melchert, R.B., Hauer-Jensen, M., and Kennedy, R.H. Hyperhomocysteinemia leads to pathological ventricular hypertrophy in normotensive rats. Am J Physiol Heart Circ Physiol 285, H679, 2003.
35. Schultheiss, D., Gabouev, A.I., Cebotari, S., Tudorache, I., Walles, T., Schlote, N., Wefer, J., Kaufmann, P.M., Haverich, A., Jonas, U., Stief, C.G., and Mertsching, H. Biological vascularized matrix for bladder tissue engineering: matrix preparation, reseeding technique and short-term implantation in a porcine model. J Urol 173, 276, 2005.
36. Cortiella, J., Niles, J., Cantu, A., Brettler, A., Pham, A., Vargas, G., Winston, S., Wang, J., Walls, S., and Nichols, J.E. Influence of acellular natural lung matrix on murine embryonic stem cell differentiation and tissue formation. Tissue Eng Part A 16, 2565, 2010.
37. Wang, Y., Cui, C.B., Yamauchi, M., Miguez, P., Roach, M., Malavarca, R., Costello, M.J., Cardinale, V., Wauthier, E., Barbier, C., Gerber, D.A., Alvaro, D., and Reid, L.M. Lineage restriction of human hepatic stem cells to mature fates is made efficient by tissue-specific biomatrix scaffolds. Hepatology 53, 293, 2011.
38. van Dijk, A., Niessen, H.W., Zandieh Doulabi, B., Visser, F.C., and van Milligen, F.J. Differentiation of human adipose-derived stem cells towards cardiomyocytes is facilitated by laminin. Cell Tissue Res 334, 457, 2008.
39. Suzuki, S., Narita, Y., Yamawaki, A., Murase, Y., Satake, M., Mutsuga, M., Okamoto, H., Kagami, H., Ueda, M., and Ueda, Y. Effects of extracellular matrix on differentiation of human bone marrow-derived mesenchymal stem cells into smooth muscle cell lineage: Utility for cardiovascular tissue engineering. Cells Tissues Organs 191, 269, 2010.
40. Jacot, J.G., Kita-Matsuo, H., Wei, K.A., Chen, H.S., Omens, J.H., Mercola, M., and McCulloch, A.D. Cardiac myocyte force development during differentiation and maturation. Ann N Y Acad Sci 11, 88121, 2010.