Detection of streptococcus pneumoniae and identification of pneumococcal serotypes by real-time polymerase chain reaction using blood samples from Italian children ≤5 years of age with community-acquired pneumonia

Microbial Drug Resistance

Volumn 17, Issue 3, 2011, Pages 419-424

  Marchese, Anna ^a   Esposito, Susanna ^b   Coppo, Erika ^a   Rossi, Giovanni A ^c   Tozzi, Alberto ^d   Romano, Mariateresa ^d   Da Dalt, Liviana ^e   Schito, Gian Carlo ^a   Principi, Nicola ^b  

^a UNIVERSITY OF GENOA   (Italy)

^b UNIVERSITY OF MILAN   (Italy)

^c G GASLINI INSTITUTE   (Italy)

^d BAMBINO GESÙ CHILDREN S HOSPITAL   (Italy)

^e UNIVERSITY OF PADOVA   (Italy)

Author keywords

[No Author keywords available]

Indexed keywords

AUTOLYSIN; BACTERIAL DNA; PNEUMOCOCCUS VACCINE;

ARTICLE; BACTERIAL GENE; BACTERIAL STRAIN; BACTERIUM DETECTION; BACTERIUM IDENTIFICATION; BACTERIUM ISOLATE; BLOOD SAMPLING; CHILD; CLINICAL ARTICLE; COMMUNITY ACQUIRED PNEUMONIA; CONTROLLED STUDY; GROUPS BY AGE; HUMAN; ITALY; NONHUMAN; PRESCHOOL CHILD; PRIORITY JOURNAL; REAL TIME POLYMERASE CHAIN REACTION; SENSITIVITY AND SPECIFICITY; SEROTYPE; STREPTOCOCCUS PNEUMONIAE; VALIDATION STUDY;

ADULT; CHILD, PRESCHOOL; COMMUNITY-ACQUIRED INFECTIONS; HUMANS; INCIDENCE; ITALY; PNEUMOCOCCAL INFECTIONS; PNEUMOCOCCAL VACCINES; PNEUMONIA, PNEUMOCOCCAL; REAL-TIME POLYMERASE CHAIN REACTION; SENSITIVITY AND SPECIFICITY; SEROTYPING; STREPTOCOCCUS PNEUMONIAE; VACCINES, CONJUGATE;

STREPTOCOCCUS PNEUMONIAE;

EID: 80052214526     PISSN: 10766294     EISSN: 19318448     Source Type: Journal    
DOI: 10.1089/mdr.2011.0031     Document Type: Article

References (28)

1. American Academy of Pediatrics Committee on Infectious Diseases. 2010. Recommendations for the prevention of Streptococcus pneumoniae infections in infants and children: Use of 13-valent pneumococcal conjugate vaccine (PCV13) and pneumococcal polysaccharide vaccine (PPSV23). Pediatrics 126:186-190.
2. Arbique, J.C., C. Poyart, P. Trieu-Cuot, G. Quesne, G. Carvalho Mda, A.G. Steigerwalt, R.E. Morey, D. Jackson, R.J. Davidson, and R.R. Facklam. 2004. Accuracy of phenotypic and genotypic testing for identification of Streptococcus pneumoniae and description of Streptococcus pseudopneumoniae sp. nov. J. Clin. Microbiol. 42:4686-4696. (Pubitemid 39362390)
3. Azzari, C., M. Cortimiglia, M. Moriondo, C. Canessa, F. Lippi, F. Ghiori, L. Becciolini, M. de Martino, and M. Resti. 2011. Pneumococcal DNA is not detectable in the blood of healthy carrier children. J. Med. Microbiol. doi: 10.1033/jmm.0.028357-0
4. Azzari, C., M. Moriondo, G. Indolfi, M. Cortimiglia, C. Canessa, L. Becciolini, F. Lippi, M. De Martino, and M. Resti. 2010. Realtime PCR is more sensitive than multiplex PCR for diagnosis and serotyping in children with culture negative pneumococcal invasive disease. PLoS One 5:e9282.
5. Campbell, S.G., T.J. Marrie, R. Anstey, G. Dikinson, and S. Ackroyd-Stolarz. 2003. The contribution of blood cultures to the clinical management of adult patients admitted to the hospital with community-acquired pneumonia: A prospective observational study. Chest 123:1142-1150. (Pubitemid 36444372)
6. Centers for Disease Control and Prevention. 2010. Invasive pneumococcal disease in young children before licensure of 13-valent pneumococcal conjugate vaccine: United States, 2007. MMWR Morb. Mortal. Wkly. Rep. 59:253-257.
7. Croucher, N.J., S.R. Harris, C. Fraser, M.A. Quail, J. Burton, M. Van Der Linden, L. McGee, A. Von Gottberg, J.H. Song, K.S. Ko, B. Pichon, S. Baker, C.M. Parry, L.M. Lambertsen, D. Shahinas, D.R. Pillai, T.J. Mitchell, G. Dougan, A. Tomasz, K.P. Klugman, J. Parkhill, W.P. Hanage, and S.D. Bentley. 2011. Rapid pneumococcal evolution in response to clinical interventions. Science 331:430-434.
8. Dagan, R. 2009. Serotype replacement in perspective. Vaccine 27 Suppl 3:C22-C24.
9. Hanage, W.P., C.J. Bishop, S.S. Huang, A.E. Stevenson, S.I. Pelton, M. Lipsitch, and J.A. Finkelstein. 2010. Carried Pneumococci in Massachusetts children: The contribution of clonal expansion and serotype switching. Pediatr. Infect. Dis. J. 30:302-308.
10. Henrichsen, J. 1999. Typing of Streptococcus pneumoniae: Past, present, and future. Am. J. Med. 107:50S-54S. (Pubitemid 29372761)
11. Hicks, L.A., L.H. Harrison, B. Flannery, J.L. Hadler, W. Schaffner, A.S. Craig, D. Jackson, A. Thomas, B. Beall, R. Lynfield, A. Reingold, M.M. Farley, and C.G. Whitney. 2007. Incidence of pneumococcal disease due to nonpneumococcal conjugate vaccine (PCV7) serotypes in the United States during the era of widespread PCV7 vaccination, 1998-2004. J. Infect. Dis. 196:1346-1354. (Pubitemid 351412007)
12. Hoorfar, J., B. Malorny, A. Abdulmawjood, N. Cook, M. Wagner, and P. Fach. 2004 Practical considerations in design of internal amplification controls for diagnostic PCR assays. J. Clin. Microbiol. 42:1863-1868. (Pubitemid 38637794)
13. Kee, C., D.M. Fatovich, S. Palladino, I.D. Kay, T.M. Pryce, J. Flexman, R. Murray, and G.W. Waterer. 2010. Specificity of a quantitative real-time polymerase chain reaction assay for the detection of invasive pneumococcal disease: Identifying Streptococcus pneumoniae using quantitative polymerase chain reaction. Chest 137:243-244.
14. Korppi, M., M. Leinonen, and O. Ruuskanen. 2008. Pneumococcal serology in children's respiratory infections. Eur. J. Clin. Microbiol. Infect. Dis. 27:167-175.
15. Marchese, A., L. Gualco, I. Cochetti, M.P. Montanari, A.M. Speciale, S.R. Musumeci, P.E. Varaldo, G. Nicoletti, and G.C. Schito. 2005. Antibiotic susceptibility and serotype distribution in Streptococcus pneumoniae circulating in Italy: Results of the SEMPRE surveillance study (2000-2002). Int. J. Antimicrob. Agents. 26:138-145. (Pubitemid 41021966)
16. Marchese, A., E. Tonoli, G. Balistreri, E.A. Debbia, and G.C. Schito. 2000. Antibiotic susceptibility patterns and serotypes of antibiotic resistant and/or invasive Streptococcus pneumoniae strains circulating in Italy. Microb. Drugs Resist. 6:163-170.
17. Michelow, I.C., J. Lozano, K. Olsen, C. Goto, N.K. Rollins, F. Ghaffar, V. Rodriguez-Cerrato, M. Leinonen, and G.H. McCracken Jr. 2002. Diagnosis of Streptococcus pneumoniae lower respiratory infection in hospitalized children by culture, polymerase chain reaction, serological testing, and urinary antigen detection. Clin. Infect. Dis. 34:E1-E11.
18. Murray, P.R. 2007. Manual of Clinical Microbiology. 9th Edition. ASM Press, Washington, DC.
19. Pai, R., R.E. Gertz, and B. Beall. 2006. Sequential multiplex PCR approach for determining capsular serotypes of Streptococcus pneumoniae isolates. J. Clin. Microbiol. 44:124-131. (Pubitemid 43100590)
20. Park, H.K., H.J. Lee, and W. Kim. 2010. Real-time PCR assays for the detection and quantification of Streptococcus pneumoniae. FEMS Microbiol. Lett. 310:48-53.
21. Park, S.Y., C.A. Van Beneden, T. Pilishvili, M. Martin, R.R. Facklam, and C.G. Whitney. 2010. Active Bacterial Core Surveillance Team. Invasive pneumococcal infections among vaccinated children in the United States. J. Pediatr. 156:478.e2-483.e2.
22. Pilishvili, T., C. Lexau, M.M. Farley, J. Hadler, L.H. Harrison, N.M. Bennett, A. Reingold, A. Thomas, W. Schaffner, A.S. Craig, P.J. Smith, B.W. Beall, C.G. Whitney, and M.R. Moore. 2010. Active Bacterial Core Surveillance/ Emerging Infections Program Network. Sustained reductions in invasive pneumococcal disease in the era of conjugate vaccine. J. Infect. Dis. 201:32-41.
23. Reinert, R., M.R. Jacobs, and S.L. Kaplan. 2010. Pneumococcal disease caused by serotype 19A: Review of the literature and implications for future vaccine development. Vaccine 28:4249-4259.
24. Resti, M., A. Micheli, M. Moriondo, L. Becciolini, M. Cortimiglia, C. Canessa, G. Indolfi, E. Bartolini, M. De Martino, and C. Azzari. 2009. Comparison of the effect of antibiotic treatment on the possibility of diagnosing invasive pneumococcal disease by culture or molecular methods: A prospective, observational study of children and adolescents with proven pneumococcal infection. Clin. Ther. 31:1266-1273.
25. Resti, M., M. Moriondo, M. Cortimiglia, G. Indolfi, C. Canessa, L. Becciolini, E. Bartolini, F.M. Benedictis, M. De Martino, and C. Azzari; Italian Group for the Study of Invasive Pneumococcal Disease. 2010. Community-acquired bacteremic pneumococcal pneumonia in children: Diagnosis and serotyping by real-time polymerase chain reaction using blood samples. Clin. Infect. Dis. 51:1042-1049.
26. Sheppard, C.L., T.G. Harrison, R. Morris, A. Hogan, and R.C. George. 2004. Autolysin-targeted LightCycler assay including internal process control for detection of Streptococcus pneumoniae DNA in clinical samples. J. Med. Microbiol. 53:189-195. (Pubitemid 38340117)
27. Tarragó, D., A. Fenoll, D. Sánchez-Tatay, L.A. Arroyo, C. Muñoz-Almagro, C. Esteva, W.P. Hausdorff, J. Casal, and I. Obando. 2008. Identification of pneumococcal serotypes from culture-negative clinical specimens by novel real-time PCR. Clin. Microbiol. Infect. 14:828-834.
28. World Health Organization (WHO). 2007. Pneumococcal conjugate vaccine for childhood immunization-WHO position paper. Wkly. Epidemiol. Rec. 82:93-104.