Oral and fecal Campylobacter concisus strains perturb barrier function by apoptosis induction in HT-29/B6 intestinal epithelial cells

PLoS ONE

Volumn 6, Issue 8, 2011, Pages

  Nielsen, Hans Linde ^a   Nielsen, Henrik ^a   Ejlertsen, Tove ^a   Engberg, Jørgen ^b   Günzel, Dorothee ^c   Zeitz, Martin ^c   Hering, Nina A ^c   Fromm, Michael ^c   Schulzke, Jörg Dieter ^c   Bücker, Roland ^c  

^a AALBORG UNIVERSITY HOSPITAL   (Denmark)

^b SLAGELSE HOSPITAL   (Denmark)

^c CHARITÉ UNIVERSITÄTSMEDIZIN BERLIN   (Germany)

Author keywords

[No Author keywords available]

Indexed keywords

CAMPTOTHECIN; CASPASE 3; CLAUDIN 5; DEXTRAN; FLUORESCEIN; LACTATE DEHYDROGENASE; PROTEIN ZO1;

ADULT; APICAL MEMBRANE; APOPTOSIS; ARTICLE; BACTERIAL INFECTION; BACTERIAL STRAIN; BASOLATERAL MEMBRANE; CAMPYLOBACTER; CAMPYLOBACTER CONCISUS; CHILD; CLINICAL ARTICLE; CONTROLLED STUDY; CYTOTOXICITY; ELECTRIC RESISTANCE; ENZYME ACTIVATION; ENZYME RELEASE; FECES; FEMALE; GENE CONTROL; HUMAN; HUMAN CELL; INTESTINE EPITHELIUM CELL; MALE; MOLECULAR SIZE; MOUTH; MUCUS; NONHUMAN; PERMEABILITY BARRIER; PRESCHOOL CHILD; PROTEIN EXPRESSION; PROTEIN LOCALIZATION; STRAIN DIFFERENCE; TIGHT JUNCTION; CELL LINE; DIARRHEA; EPITHELIUM CELL; IMMUNOLOGY; INTESTINE MUCOSA; MICROBIOLOGY; PATHOGENICITY; PATHOLOGY; PERMEABILITY;

CAMPYLOBACTER CONCISUS;

APOPTOSIS; CAMPYLOBACTER; CELL LINE; DIARRHEA; EPITHELIAL CELLS; HUMANS; INTESTINAL MUCOSA; PERMEABILITY; TIGHT JUNCTIONS;

EID: 80052001771     PISSN: None     EISSN: 19326203     Source Type: Journal    
DOI: 10.1371/journal.pone.0023858     Document Type: Article

References (27)

1. Newell DG, (2005) Campylobacter concisus: an emerging pathogen? Eur J Gastroenterol Hepatol 17 (10): 1013-1014.
2. Chaban B, Ngeleka M, Hill JE, (2010) Detection and quantification of 14 Campylobacter species in pet dogs reveals an increase in species richness in feces of diarrheic animals. BMC Microbiol 10: 73.
3. Zhang L, Budiman V, Day AS, Mitchell H, Lemberg DA, et al. (2010) Isolation and detection of Campylobacter concisus from saliva of healthy individuals and patients with inflammatory bowel disease. J Clin Microbiol 48 (8): 2965-2967.
4. Aabenhus R, Permin H, On SL, Andersen LP, (2002) Prevalence of Campylobacter concisus in diarrhoea of immunocompromised patients. Scand J Infect Dis 34 (4): 248-252.
5. Lastovica AJ, (2009) Clinical relevance of Campylobacter concisus isolated from pediatric patients. J Clin Microbiol 47 (7): 2360.
6. Aabenhus R, Stenram U, Andersen LP, Permin H, Ljungh A, (2008) First attempt to produce experimental Campylobacter concisus infection in mice. World J Gastroenterol 14 (45): 6954-6959.
7. Istivan TS, Coloe PJ, Fry BN, Ward P, Smith SC, (2004) Characterization of a haemolytic phospholipase A(2) activity in clinical isolates of Campylobacter concisus. J Med Microbiol 53 (Pt 6): 483-493.
8. Kaakoush NO, Man SM, Lamb S, Raftery MJ, Wilkins MR, et al. (2010) The secretome of Campylobacter concisus. FEBS J 277 (7): 1606-1617.
9. Kalischuk LD, Inglis GD, (2011) Comparative genotypic and pathogenic examination of Campylobacter concisus isolates from diarrheic and non-diarrheic humans. BMC Microbiol 11: 53.
10. Engberg J, Bang DD, Aabenhus R, Aarestrup FM, Fussing V, et al. (2005) Campylobacter concisus: an evaluation of certain phenotypic and genotypic characteristics. Clin Microbiol Infect 11 (4): 288-295.
11. Man SM, Kaakoush NO, Leach ST, Nahidi L, Lu HK, et al. (2010) Host Attachment, Invasion, and Stimulation of Proinflammatory Cytokines by Campylobacter concisus and Other Non-Campylobacter jejuni Campylobacter Species. J Infect Dis 202 (12): 1855-1865.
12. Bücker R, Troeger H, Kleer J, Fromm M, Schulzke JD, (2009) Arcobacter butzleri induces barrier dysfunction in intestinal HT-29/B6 cells. J Infect Dis 200 (5): 756-764.
13. Bojarski C, Gitter AH, Bendfeldt K, Mankertz J, Schmitz H, et al. (2001) Permeability of human HT-29/B6 colonic epithelium as a function of apoptosis. J Physiol 535 (Pt 2): 541-552.
14. Soderholm JD, Streutker C, Yang PC, Paterson C, Singh PK, et al. (2004) Increased epithelial uptake of protein antigens in the ileum of Crohn's disease mediated by tumour necrosis factor alpha. Gut 53 (12): 1817-1824.
15. Kalischuk LD, Inglis GD, Buret AG, (2007) Strain-dependent induction of epithelial cell oncosis by Campylobacter jejuni is correlated with invasion ability and is independent of cytolethal distending toxin. Microbiology 153 (Pt 9): 2952-2963.
16. Florian P, Schöneberg T, Schulzke JD, Fromm M, Gitter AH, (2002) Single-cell epithelial de-fects close rapidly by an actinomyosin purse string mechanism with functional tight junctions. J Physiol 545 (Pt 2): 485-499.
17. Bücker R, Krug SM, Rosenthal R, Günzel D, Fromm A, et al. (2011) Aerolysin from Aeromonas hydrophila perturbs tight junction integrity and cell lesion repair in intestinal epithelial HT-29/B6 cells. J Infect Dis. In Press.
18. Gradel KO, Nielsen HL, Schonheyder HC, Ejlertsen T, Kristensen B, et al. (2009) Increased short- and long-term risk of inflammatory bowel disease after salmonella or campylobacter gastroenteritis. Gastroenterology 137 (2): 495-501.
19. Engberg J, On SL, Harrington CS, Gerner-Smidt P, (2000) Prevalence of Campylobacter, Arcobacter, Helicobacter, and Sutterella spp. in human fecal samples as estimated by a reevaluation of isolation methods for Campylobacters. J Clin Microbiol 38 (1): 286-291.
20. Chaban B, Musil KM, Himsworth CG, Hill JE, (2009) Development of cpn60-based real-time quantitative PCR assays for the detection of 14 Campylobacter species and application to screening of canine fecal samples. Appl Environ Microbiol 75 (10): 3055-3061.
21. Troeger H, Richter JF, Beutin L, Günzel D, Dobrindt U, et al. (2007) Escherichia coli alpha-haemolysin induces focal leaks in colonic epithelium: a novel mechanism of bacterial translocation. Cell Microbiol 9 (10): 2530-2540.
22. Kreusel KM, Fromm M, Schulzke JD, Hegel U, (1991) Cl- secretion in epithelial monolayers of mucus-forming human colon cells (HT-29/B6). Am J Physiol 261 (4 Pt 1): C574-C582.
23. Epple HJ, Kreusel KM, Hanski C, Schulzke JD, Riecken EO, et al. (1997) Differential stimulation of intestinal mucin secretion by cholera toxin and carbachol. Pflugers Arch 433 (5): 638-647.
24. Schulzke JD, Fromm M, Bentzel CJ, Zeitz M, Menge H, et al. (1992) Ion transport in the experimental short bowel syndrome of the rat. Gastroenterology 102 (2): 497-504.
25. Amasheh S, Schmidt T, Mahn M, Florian P, Mankertz J, et al. (2005) Contribution of claudin-5 to barrier properties in tight junctions of epithelial cells. Cell Tissue Res 321 (1): 89-96.
26. Madara JL, Stafford J, (1989) Interferon-gamma directly affects barrier function of cultured intestinal epithelial monolayers. J Clin Invest 83: 724-727.
27. Livak KJ, Schmittgen TD, (2001) Analysis of relative gene expression datausing real-time quantitative PCR and the 2(-ΔΔC(T)) method. Methods 25: 402-408.