Application of cycleave PCR to the detection of a point mutation (F167Y) in the β2-tubulin gene of Fusarium graminearum

Pest Management Science

Volumn 67, Issue 9, 2011, Pages 1124-1128

  Hou, Yiping ^a   Luo, Qingquan ^a,b   Chen, Changjun ^a   Zhou, Mingguo ^a  

^a NANJING AGRICULTURAL UNIVERSITY   (China)

^b Shanghai Institute of Landscape Gardening Science   (China)

Author keywords

2 tubulin; Fusarium graminearum; Real time PCR; Resistance of carbendazim

Indexed keywords

FUNGAL PROTEIN; FUNGICIDE; PRIMER DNA; TUBULIN;

BIOASSAY; CARBENDAZIM; DETECTION METHOD; DISEASE RESISTANCE; FLUORESCENCE; FREQUENCY ANALYSIS; FUNGUS; GENE EXPRESSION; GERMINATION; MUTATION; POLYMERASE CHAIN REACTION; SPORE;

Β2-TUBULIN; ANTIFUNGAL RESISTANCE; ARTICLE; DRUG EFFECT; EVALUATION STUDY; FUSARIUM; FUSARIUM GRAMINEARUM; GENETICS; METHODOLOGY; POINT MUTATION; REAL TIME POLYMERASE CHAIN REACTION; REAL-TIME PCR; RESISTANCE OF CARBENDAZIM;

Β2-TUBULIN; FUSARIUM GRAMINEARUM; REAL-TIME PCR; RESISTANCE OF CARBENDAZIM;

DNA PRIMERS; DRUG RESISTANCE, FUNGAL; FUNGAL PROTEINS; FUNGICIDES, INDUSTRIAL; FUSARIUM; POINT MUTATION; REAL-TIME POLYMERASE CHAIN REACTION; TUBULIN;

CHINA;

GIBBERELLA ZEAE;

EID: 80051933549     PISSN: 1526498X     EISSN: 15264998     Source Type: Journal    
DOI: 10.1002/ps.2161     Document Type: Article

References (19)

1. Luo QQ, Xu JQ, Hou YP, Chen CJ, Wang JX Zhou MG, PIRA-PCR for detection of Fusarium graminearum genotypes with moderate resistance to carbendazim. Plant Pathol 58: 882-887 (2009).
2. Wang JX, Zhou MG, Lu YJ Ye ZY, Dynamics of resistant population of Fusarium graminearum to carbendazim and substitutable fungicide screening. J Nanjing Agric Univ 25: 43-47 (2002).
3. Bai G Shaner G, Scab of wheat: prospects of control. Plant Dis 78: 760-766 (1994).
4. Yao J Lu W, Research advances in wheat breeding for scab resistance in China. Jiangsu J Agric Sci 16: 242-248 (2000).
5. Chen CJ, Yu JJ, Bi CW, Zhang YN, Xu JQ, Wang JX, et al, Mutations in a β-tubulin confer resistance of Gibberella zeae to benzimidazole fungicides. Phytopathology 99: 1403-1411 (2009).
6. Ma Z Michailides TJ, An allele-specific PCR assay for detecting azoxystrobin-resistant Alternaria isolates from pistachio in California. J Phytopathol 152: 118-121 (2004).
7. Piddock LJV, Ricci V, McLaren I Griggs DJ, Role of mutations in the gyrA and parC gene of 196 nalidixic acidresistant Salmonella serotypes isolated from animals. J Antimicrobial Chemother 41: 635-642 (1998).
8. Zirnstein G., Swaminathan YLB Angulo F, Ciprofloxacin resistance in Campylobacter jejuni isolates: detection of gyrA resistance mutations by mismatch amplification mutation assay PCR and DNA sequence analysis. J Clin Microbiol 37: 3276-3280 (1999).
9. Eaves DJ, Liebana E, Woodward MJ Piddock LJV, Detection of gyrA mutations in quinolone-resistant Salmonella enterica by denaturing high-performance liquid chromatography. J Clin Microbiol 40: 4121-4125 (2002).
10. Ma ZH, Yoshimura MA, Holtz BA Michailides TJ, Characterization and PCR-based detection of benzimidazole-resistant isolates of Monilinia laxa in California. Pest Manag Sci 61: 449-457 (2005).
11. Saito K, Miyake S, Moriya H, Yamazaki M, Itoh F, Imai K, et al, Detection of the four sequence variations of MDR1 gene using TaqMan-MGB probe based real-time PCR and haplotype analysis in healthy Japanese subjects. Clin Biochem 36: 511-518 (2003).
12. Walker RA, Saunders N, Lawson AJ, Lindsay EA, Dassama M, Ward LR, et al, Use of the LightCycler gyrA mutation assay for the rapid identification of mutations conferring decreased susceptibility to ciprofloxacin in multiresistant Salmonella enterica serotype typhimurium DT104 isolates. J Clin Microbiol 39: 1443-1448 (2001).
13. Esaki H, Noda K, Otsuki N, Kojima A, Asai T, Tamura Y, et al, Rapid detection of quinolone-resistant Salmonella by real time SNP genotyping. J Microbiol Meth 58: 131-134 (2004).
14. Yatabe Y, Hida T, Horio Y, Kosaka T, Takahashi T Mitsudomi T, A rapid, sensitive assay to detect EGFR mutation in small biopsy specimens from lung cancer. J Molec Diagnostis 8: 335-341 (2006).
15. Yoshida Y Kubo S, Y-SNP and Y-STR analysis in a Japanese population. Legal Med 10: 243-252 (2008).
16. Yuan SK Zhou MG, A major gene for resistance to carbendazim in field isolates of Gibberella zeae. Can J Plant Pathol 27: 58-63 (2005).
17. Chen CJ, Wang JX, Luo QQ, Yuan SK Zhou MG, Characterization and fitness of carbendazim-resistant strains of Fusarium graminearum (wheat scab). Pest Manag Sci 63: 1201-1207 (2007).
18. Nicholson P Parry DW, Development and use of a PCR assay to detect Rhizoctonia cerealis, the cause of sharp eyespot of wheat. Plant Pathol 45: 872-883 (1996).
19. Zhou MG, Ye ZY Liu JF, Progress of fungicide resistance research. J Nanjing Agric Univ 17: 33-41 (1994).