Engineering of an E. coli outer membrane protein FhuA with increased channel diameter

Journal of Nanobiotechnology

Volumn 9, Issue , 2011, Pages

  Krewinkel, Manuel ^a   Dworeck, Tamara ^a   Fioroni, Marco ^a  

^a RWTH AACHEN UNIVERSITY   (Germany)

Author keywords

Channel proteins; FhuA; HRP; Liposomes; Nanocontainers; Protein engineering; TMB Assay

Indexed keywords

CHANNEL PROTEINS; FHUA; HRP; NANOCONTAINERS; PROTEIN ENGINEERING; TMB-ASSAY;

AMINO ACIDS; BIOLOGICAL MEMBRANES; BIOSYNTHESIS; CIRCULAR DICHROISM SPECTROSCOPY; DICHROISM; ESCHERICHIA COLI; GENETIC ENGINEERING; INDUSTRIAL APPLICATIONS; LIPOSOMES; PHOSPHOLIPIDS;

PROTEINS;

FERRIC HYDROXAMATE UPTAKE PROTEIN COMPONENT A; HORSERADISH PEROXIDASE; LIPOSOME; NANOCHANNEL; TETRAMETHYLBENZIDINE; 3,3',5,5'-TETRAMETHYLBENZIDINE; BENZIDINE DERIVATIVE; ESCHERICHIA COLI PROTEIN; FHUA PROTEIN, E COLI; ION CHANNEL; OUTER MEMBRANE PROTEIN;

AMINO ACID SEQUENCE; AMINO TERMINAL SEQUENCE; ARTICLE; BETA SHEET; CIRCULAR DICHROISM; CONTROLLED STUDY; ESCHERICHIA COLI; GENETIC ENGINEERING; NONHUMAN; PROTEIN FOLDING; PROTEIN VARIANT; STRUCTURE ANALYSIS; DIFFUSION; GENETICS; METABOLISM; MOLECULAR GENETICS; NUCLEOTIDE SEQUENCE; PROTEIN SECONDARY STRUCTURE;

ARMORACIA RUSTICANA;

AMINO ACID SEQUENCE; BACTERIAL OUTER MEMBRANE PROTEINS; BASE SEQUENCE; BENZIDINES; CIRCULAR DICHROISM; DIFFUSION; ESCHERICHIA COLI; ESCHERICHIA COLI PROTEINS; GENETIC ENGINEERING; HORSERADISH PEROXIDASE; ION CHANNELS; MOLECULAR SEQUENCE DATA; PROTEIN FOLDING; PROTEIN STRUCTURE, SECONDARY;

EID: 80051786355     PISSN: None     EISSN: 14773155     Source Type: Journal    
DOI: 10.1186/1477-3155-9-33     Document Type: Article

References (36)

1. Schulz G. Porins: general to specific, native to engineered passive pores. Curr Opin Struct Biol 1996, 6:485-490. 10.1016/S0959-440X(96)80113-8, 8794162.
2. Kleinschmidt JH, KT T. Secondary and tertiary structure formation of the b-barrel membrane protein OmpA is synchronized and depends on membrane thickness. J Mol Biol 2002, 324:319-330. 10.1016/S0022-2836(02)01071-9, 12441110.
3. Ramachandran R, Heuck AP, Tweten RK, Johnson AE. Structural insights into the membrane-anchoring mechanism of a cholesterol-dependent cytolysin. Nat Struct Biol 2002, 9:823-827.
4. Wimley C. The versatile beta-barrel membrane protein. Curr Opin Struc Biol 2003, 13:404-411.
5. Ye J, van den Berg B. Crystal structure of the bacterial nucleoside transporter Tsx. EMBO J 2004, 23:3187-3195. 10.1038/sj.emboj.7600330, 514505, 15272310.
6. Cowan SW, Garavito RM, Jansonius JN, Jenkins JA, Karlsson R, Konig N, Pai EF, Pauptit RA, Rizkallah PJ, Rosenbach JP. The structure of OmpF porin in a tetragonal crystal form. Structure 1995, 3:1041-1050. 10.1016/S0969-2126(01)00240-4, 8589999.
7. Nallani M, Benito S, Onaca O, Graff A, Lindemann M, Winterhalter M, Meier W, Schwaneberg U. A nanocompartment system (synthosome) designed for biotechnological applications. J Biotechnol 2006, 123:50-59. 10.1016/j.jbiotec.2005.10.025, 16364484.
8. Ranquin A, Versees W, Meier W, Steyaert J, Van Gelder P. Therapeutic nanoreactors: combining chemistry and biology in a novel triblock copolymer drug delivery system. Nano Lett 2005, 5:2220-2224. 10.1021/nl051523d, 16277457.
9. Surrey T, Schmid A, Jähnig F. Folding and membrane insertion of the trimeric beta-barrel protein OmpF. Biochemistry 1996, 35:2283-2288. 10.1021/bi951216u, 8652568.
10. Hoffmann H, Fischer E, Schwarz H, Braun V. Overproduction of the proFhuA outer membrane receptor protein of Escherichia coli K-12: isolation, properties, and immunocytochemical localization at the inner side of the cytoplasmic membrane. Arch Microbiol 1986, 145:334-341. 10.1007/BF00470867, 3539058.
11. Ferguson A, Braun V, Fiedler H-P, Coulton J, Diederichs K, Welte W. Crystal structure of the antibiotic albomycin in complex with the outer membrane transporter FhuA. Prot Sci 2000, 9:956-963.
12. Ferguson A, Hofmann E, Coulton J, Diederichs K. Siderophore-mediated iron transport: crystal structure bound lipopolysaccharide. Science 1998, 282:2215-2220.
13. Braun M, Killmann H, Maier E, Benz R, Braun V. Diffusion through channel derivatives of the Escherichia coli FhuA transport protein. Eur J Biochem 2002, 269:4948-4959. 10.1046/j.1432-1033.2002.03195.x, 12383253.
14. Onaca O, Sarkar P, Roccatano D, Friedrich T, Hauer B, Grzelakowski M, Güven A, Fioroni M, Schwaneberg U. Functionalized nanocompartments (Synthosomes) with a reduction-triggered release system. Ang Chem Int Ed 2008, 47:7029-7031.
15. Güven A, Fioroni M, Hauer B, Schwaneberg U. Molecular understanding of sterically controlled compound release through an engineered channel protein (FhuA). J Nanobiotech 2010, 8(14).
16. Muhammad N, Dworeck T, Fioroni M, Schwaneberg U. Engineering of the E. coli Outer Membrane Protein FhuA to overcome the Hydrophobic Mismatch in Thick Polymeric Membranes. J Nanobiotech 2011, 9(8).
17. Baumeister B, Matile S. Rigid-rod beta-barrels as lipocalin models: probing confined space by carotenoid encapsulation. Chem - Eur J 2000, 6:1739-1749.
18. Sakai N, Matile S. Synthetic multifunctional pores: lessons from rigid-rod beta-barrels. Chem Commun 2003, 2514-2523.
19. Schwab PFH, Levin MD, Michl J. Molecular rods. 1. Simple axial rods. Chem Rev 1999, 99:1863-1934. 10.1021/cr970070x, 11849014.
20. Litvinchuk S, Bollot G, Mareda J, Som A, Ronan D, Shah MR, Perrottet P, Sakai N, Matile S. Thermodynamic and kinetic stability of synthetic multifunctional rigid-rod β-barrel pores: evidence for supramolecular catalysis. J Am Chem Soc 2004, 126:10067-10075. 10.1021/ja0481878, 15303883.
21. Kumaki J, Yashima E, Bollot G, Mareda J, Litvinchuk S, Matile S. AFM snapshots of synthetic multifunctional pores with polyacetylene blockers: Pseudorotaxanes and template effects. Angew Chem, Int Ed 2005, 44:6154-6157.
22. Litvinchuk S, Matile S. Blockage of rigid-rod beta-barrel pores by rigid-rod R-helix mimics. Supramol Chem 2005, 17:135-139.
23. Cantor CR, Schimmel PR. The conformation of biological macromoleculs 1980, New York: W. H. Freeman and Company.
24. Dworeck T, Petri AK, Muhammad N, Fioroni M, Schwaneberg U. FhuA Deletion Variant del1-159 Overexpression in Inclusion Bodies and Refolding with Polyethylene-Poly(ethylene glycol) Diblock Copolymer. Prot Expr Purif 2011, 77(1):75-79.
25. Boulanger P, le Marie M, Bonhivers M, Dubois S, Desmadril S, Letellier L. Purification and structural and functional characterization of FhuA, a transporter of the Escherichia coli outer membrane. Biochemistry 1996, 35:14216-14224. 10.1021/bi9608673, 8916906.
26. Andrade M, Chacón P, Merelo J, Morán F. Evaluation of secondary structure of proteins from UV circular dichroism using an unsupervised learning neural network. Prot Eng 1993, 6:383-390.
27. Josephy P, Eling T, Mason R. The horseradish peroxidase-catalyzed oxidation of 3,5,3',5'-tetramethylbenzidine. Free radical and chargetransfer complex intermediates. J of Biol Chem 1982, 257:3669-3675.
28. Josephy P, Eling T, Mason R. The horseradish peroxidase-catalyzed oxidation of 3,5,3',5'-tetramethylbenzidine. Free radical and charge-transfer complex intermediates. J Biol Chem 1982, 257:3669-3675.
29. Prilipov A, Phale P, Koebnik R, Widmer C, Rosenbusch J. Identification and characterization of two quiescent porin genes, nmpC and ompN, in Escherichia coli B. J Bacteriol 1998, 180:3388-3392. 107294, 9642192.
30. Onoda T, Enokizono J, Kaya H, Oshima A, Freestone P, Norris V. Effects of calcium and calcium chelators on growth and morphology of Escherichia coli L-form NC-7. J Bacteriol 2000, 182(5):1419-1422. 10.1128/JB.182.5.1419-1422.2000, 94432, 10671467.
31. Locher KP, Rosenbusch JP. Oligomeric states and siderophore binding of the ligand-gated FhuA protein that forms channels across Escherichia coli outer membranes. Eur J Biochem 1997, 247(3):770-775. 10.1111/j.1432-1033.1997.t01-1-00770.x, 9288896.
32. Locher KP, Rees B, Koebnik R, Mitschler A, Moulinier L, Rosenbusch JP, Moras D. Transmembrane signaling across the ligand-gated FhuA receptor: crystal structures of free and ferrichrome-bound states reveal allosteric changes. Cell 1998, 95:771-778. 10.1016/S0092-8674(00)81700-6, 9865695.
33. Laemmli U. Cleavage of structural proteins during the assembly of the head of bacteriophage T4. Nature 1970, 227:680-685. 10.1038/227680a0, 5432063.
34. Deléage G, Geourjon C. An interactive graphic program for calculating the secondary structures content of proteins from circular dichroism spectrum. Comput Appl Biosci 1993, 2:197-199.
35. Provencher SW. An eigenfunction expansion method for the analysis of exponential decay curves. Comput Phys Commun 1982, 27:213-227.
36. Broz P, Benito SM, Saw C, Burger P, Heider H, Pfisterer M, Marsch S, Meier W, Hunziker P. Cell targeting by a generic receptor-targeted polymer nanocontainer platform. J Control Release 2005, 102:475-488. 10.1016/j.jconrel.2004.10.014, 15653165.