Presence of murine leukemia virus (MLV)-related virus gene sequences in a commercial RT-PCR reagent

Clinical Laboratory

Volumn 57, Issue 7-8, 2011, Pages 631-634

  Wolff, Dietmar ^a   Gerritzen, Andreas ^a  

^a Medizinisches Labor Bremen   (Germany)

Author keywords

Contamination; MLV related virus; PCR reagent; Real time PCR; XMRV

Indexed keywords

ARTICLE; CONTROLLED STUDY; FALSE POSITIVE RESULT; GENE AMPLIFICATION; GENE SEQUENCE; MURINE LEUKEMIA VIRUS; NONHUMAN; OBSERVATIONAL STUDY; REVERSE TRANSCRIPTION POLYMERASE CHAIN REACTION; VIRUS DETECTION; XENOTROPIC MULV RELATED VIRUS;

BLOOD; COMPUTER SYSTEMS; DNA, COMPLEMENTARY; DRUG CONTAMINATION; FALSE POSITIVE REACTIONS; GENES, GAG; HUMANS; INDICATORS AND REAGENTS; LEUKEMIA VIRUS, MURINE; REAGENT KITS, DIAGNOSTIC; REVERSE TRANSCRIPTASE POLYMERASE CHAIN REACTION; RNA, VIRAL; XENOTROPIC MURINE LEUKEMIA VIRUS-RELATED VIRUS;

GAMMARETROVIRUS; MURINE LEUKEMIA VIRUS;

EID: 80051735019     PISSN: 14336510     EISSN: None     Source Type: Journal    
DOI: None     Document Type: Article

References (16)

1. Lombardi VC, Ruscetti FW, Das Gupta J, et al. Detection of an infectious retrovirus, XMRV, in blood cells of patients with chronic fatigue syndrome. Science 2009;326:585-9.
2. Lo SC, Pripuzova N, Li B, et al. Detection of MLV-related virus gene sequences in blood of patients with chronic fatigue syndrome and healthy blood donors. Proc Natl Acad Sci USA 2010; 107:15874-9.
3. Erlwein O, Kaye S, McClure MO, et al. Failure to detect the novel retrovirus XMRV in chronic fatigue syndrome. Plos ONE 2010;5:e8519.
4. Groom, HCT, Boucherit VC, Makinson K, et al. Absence of xenotropic murine leukaemia virus-related virus in UK patients with chronic fatigue syndrome. Retrovirology 2010;7:e10.
5. Switzer WM., Hohn HJO, Zheng HQ, et al. Absence of evidence of xenotropic murine leukemia virus-related virus infection in persons with chronic fatigue syndrome and healthy controls in the United States. Retrovirology 2010; 7:57.
6. van Kuppeveld FJ, de Jong AS, Lanke KH, et al. Prevalence of xenotropic murine leukaemia virus-relared virus in patients with chronic fatigue dyndrome in the Netherlands: retrospective analysis of samples from an estab-lished cohort.BMJ 2010;340:c1018.
7. Boettger EC. Frequent contamination of Taq polymerase with DNA. Clin Chem 1990;36:1258-9.
8. Corless CE, Guiver M, Borrow R, et al.Contamination and sensitivity issues with a real-time universal 16S rRNA PCR. J Clin Microbiol 2000;38:1747-52. (Pubitemid 30253908)
9. Hughes MS, Beck LA, Skuce RA. Identification and elimation of DNA sequnces in Taq DNA polymerase. J Clin Microbiol 1994; 32:2007-8. (Pubitemid 24222288)
10. Meier A., Persing DH, Finken M, et al. Elimination of contaminating DNA within polymerase chain reactions: implications for a general approach to dection of uncultured pathogens. J Clin Microbiol 1993;31:646-52. (Pubitemid 23059882)
11. Newsome T, Bing-Jie L, Nianxiang Z, et al. Presence of bacterial phage-like DNA sequences in commercial Taq DNA polymerase reagents. J Clin Microbiol 2004;42: 2264-7. (Pubitemid 38637861)
12. Rand KH, Houck H. Taq polymerase contains bacterial DNA of unknown origin. Mol Cell Probes 1990;4:445-50.
13. Sato E, Furuta RA, Miyazawa T. An endogenous murine leukemia viral genome contaminant in a commercial RT-PCR Kit is amplified using standard primers for XMRV. Retrovirology 2010;7:110.
14. Weiss R. A cautionary tale of virus and disease. BMC Biology 2010;8:124.
15. Robinson MJ, Erlwein OW, Kaye S, et al. Mouse DNA contamination in human tissue tested for XMRV. Retrovirology 2010;7: 108.
16. Oakes B, Tai AK, Cingöz O, et al. Contamination of human DNA samples with mouse DNA can lead to false detection of XMRV-like sequences. Retrovirology 2010;7: 109.