High-throughput and cost-effective global DNA methylation assay by liquid chromatography-mass spectrometry

Analytica Chimica Acta

Volumn 703, Issue 1, 2011, Pages 58-63

  Li, Xingnan ^a   Franke, Adrian A ^a  

^a UNIVERSITY OF HAWAII CANCER CENTER   (United States)

Author keywords

5 Methyl 2 deoxycytidine; Global DNA methylation assay; Internal standard; Liquid chromatography mass spectrometry; Orbitrap MS

Indexed keywords

96-WELL FORMAT; ANALYTES; DEOXYGUANOSINE (DG); DNA EXTRACTION; DNA METHYLATION; ELECTROSPRAYS; HIGH-THROUGHPUT; INTERNAL STANDARDS; LC-ESI-MS; LC-MS/MS; LIMIT OF DETECTION; LIQUID CHROMATOGRAPHY MASS SPECTROMETRY; LIQUID CHROMATOGRAPHY-TANDEM MASS SPECTROMETRY; MULTIPLE REACTION MONITORING; ORBITRAP; ORBITRAP MS; PERIPHERAL BLOOD; REVERSE-PHASE COLUMN; SAMPLE PREPARATION; SCAN MODE;

ALKYLATION; BIOMOLECULES; CHROMATOGRAPHY; COST EFFECTIVENESS; DNA; DNA SEQUENCES; HIGH PRESSURE LIQUID CHROMATOGRAPHY; MASS SPECTROMETRY; METHYLATION;

LIQUIDS;

DEOXYGUANOSINE; METHYLATED 2' DEOXYCYTIDINE; UNCLASSIFIED DRUG;

ACCURACY; ARTICLE; BLOOD ANALYSIS; CHEMICAL REACTION; CONTROLLED STUDY; COST EFFECTIVENESS ANALYSIS; DNA DETERMINATION; DNA EXTRACTION; DNA METHYLATION; ELECTROSPRAY MASS SPECTROMETRY; FILTRATION; HIGH THROUGHPUT SCREENING; HUMAN; HYDROLYSIS; INTERMETHOD COMPARISON; LIMIT OF DETECTION; LIQUID CHROMATOGRAPHY; PRIORITY JOURNAL; QUANTITATIVE ANALYSIS; TANDEM MASS SPECTROMETRY;

CHROMATOGRAPHY, LIQUID; COST-BENEFIT ANALYSIS; DEOXYCYTIDINE; DEOXYGUANOSINE; DNA; DNA METHYLATION; HIGH-THROUGHPUT SCREENING ASSAYS; HYDROLYSIS; SPECTROMETRY, MASS, ELECTROSPRAY IONIZATION; TANDEM MASS SPECTROMETRY;

EID: 80051671417     PISSN: 00032670     EISSN: 18734324     Source Type: Journal    
DOI: 10.1016/j.aca.2011.07.014     Document Type: Article

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