Bias in bacterial diversity as a result of Nycodenz extraction from bulk soil

Soil Biology and Biochemistry

Volumn 43, Issue 10, 2011, Pages 2152-2159

  Holmsgaard, Peter N ^a   Norman, Anders ^a   Hede, Simon Chr ^c   Poulsen, Pernille H B ^a,b   Al Soud, Waleed Abu ^a   Hansen, Lars H ^a   Sørensen, Søren J ^a  

^a UNIVERSITY OF COPENHAGEN   (Denmark)

^b UNIVERSITY OF COPENHAGEN   (Denmark)

^c TECHNICAL UNIVERSITY OF DENMARK   (Denmark)

Author keywords

Amplicon pyrosequencing; Bacterial extraction; Biodiversity; Density centrifugation; Nycodenz; OTU prediction

Indexed keywords

16S RRNA; AMPLICON-PYROSEQUENCING; BACTERIAL BIODIVERSITY; BACTERIAL CELLS; BACTERIAL DIVERSITY; BACTERIAL EXTRACTION; BULK SOILS; CULTURABLE; DATABASE PROJECTS; FIELD TRIAL; INFORMATION CONCERNING; MULTI-DIMENSIONAL SCALING; NYCODENZ; OPERATIONAL TAXONOMIC UNITS; OTU PREDICTION; OVERALL EFFICIENCY; PCR AMPLIFICATION; PYROSEQUENCING; SOIL SAMPLE;

BACTERIOLOGY; BIODIVERSITY; CENTRIFUGATION; CENTRIFUGES; EXTRACTION; MATHEMATICAL MODELS; ORE PELLETS; PELLETIZING; POLYMERASE CHAIN REACTION; RNA; SOILS;

GEOLOGIC MODELS;

BACTERIUM; CENTRIFUGE; DATABASE; EFFICIENCY MEASUREMENT; EXTRACTION METHOD; NUMERICAL MODEL; POLYMERASE CHAIN REACTION; SAMPLING BIAS; SOIL ANALYSIS; SOIL MICROORGANISM; SPECIES DIVERSITY; TAXONOMY;

DENMARK;

BACTERIA (MICROORGANISMS); OTUS;

EID: 80051671305     PISSN: 00380717     EISSN: None     Source Type: Journal    
DOI: 10.1016/j.soilbio.2011.06.019     Document Type: Article

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