Purification and functional analysis of protein kinase G-1α using a bacterial expression system

Protein Expression and Purification

Volumn 79, Issue 2, 2011, Pages 271-276

  Aggarwal, Saurabh ^a   Rafikov, Ruslan ^a   Gross, Christine M ^a   Kumar, Sanjiv ^a   Pardo, Daniel ^a   Black, Stephen M ^a  

^a MEDICAL COLLEGE OF GEORGIA   (United States)

Author keywords

Cyclic GMP; Protein kinase G; Purification; Rosetta gami 2(DE3)

Indexed keywords

CYCLIC GMP; CYCLIC GMP DEPENDENT PROTEIN KINASE; HYBRID PROTEIN; HYDROGEN PEROXIDE; NITRIC OXIDE;

AFFINITY CHROMATOGRAPHY; ARTICLE; BACTERIUM TRANSFORMATION; CARDIOVASCULAR DISEASE; CELL CULTURE; CYTOLOGY; DRUG EFFECT; ENZYME ACTIVATION; ENZYMOLOGY; ESCHERICHIA COLI; GEL CHROMATOGRAPHY; GENETICS; HUMAN; ION EXCHANGE CHROMATOGRAPHY; ISOLATION AND PURIFICATION; KINETICS; METABOLISM; METHODOLOGY; MOLECULAR CLONING; OXIDATIVE STRESS; PATHOLOGY; PLASMID; SMOOTH MUSCLE FIBER; VASCULAR SMOOTH MUSCLE;

CARDIOVASCULAR DISEASES; CELLS, CULTURED; CHROMATOGRAPHY, AFFINITY; CHROMATOGRAPHY, GEL; CHROMATOGRAPHY, ION EXCHANGE; CLONING, MOLECULAR; CYCLIC GMP; CYCLIC GMP-DEPENDENT PROTEIN KINASES; ENZYME ACTIVATION; ESCHERICHIA COLI; HUMANS; HYDROGEN PEROXIDE; KINETICS; MUSCLE, SMOOTH, VASCULAR; MYOCYTES, SMOOTH MUSCLE; NITRIC OXIDE; OXIDATIVE STRESS; PLASMIDS; RECOMBINANT FUSION PROTEINS; TRANSFORMATION, BACTERIAL;

BACTERIA (MICROORGANISMS); ESCHERICHIA COLI; EUKARYOTA; HEXAPODA; PROKARYOTA;

EID: 80051600602     PISSN: 10465928     EISSN: None     Source Type: Journal    
DOI: 10.1016/j.pep.2011.05.001     Document Type: Article

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