Heterologous production, purification and characterization of enzymatically active Sindbis virus nonstructural protein nsP1

Protein Expression and Purification

Volumn 79, Issue 2, 2011, Pages 277-284

  Tomar, Shailly ^a   Narwal, Manju ^a   Harms, Etti ^c   Smith, Janet L ^b   Kuhn, Richard J ^c  

^a INDIAN INSTITUTE OF TECHNOLOGY ROORKEE   (India)

^b UNIVERSITY OF MICHIGAN MEDICAL SCHOOL   (United States)

^c PURDUE UNIVERSITY   (United States)

Author keywords

Alphavirus; Guanylyltransferase; Methyltransferase; nsP1; Purification; SINV

Indexed keywords

DETERGENT; GUANOSINE TRIPHOSPHATE; HIS HIS HIS HIS HIS HIS; HIS-HIS-HIS-HIS-HIS-HIS; HISTIDINE; MESSENGER RIBONUCLEIC ACID GUANYLYLTRANSFERASE; METHYLTRANSFERASE; NSP1 PROTEIN, SINDBIS VIRUS; NUCLEOTIDYLTRANSFERASE; OLIGOPEPTIDE; RECOMBINANT PROTEIN; VIRUS PROTEIN;

AFFINITY CHROMATOGRAPHY; ALPHAVIRUS INFECTION; ARTICLE; BACTERIUM TRANSFORMATION; CHEMISTRY; CIRCULAR DICHROISM; DRUG EFFECT; ENZYME ACTIVATION; ENZYME SPECIFICITY; ENZYMOLOGY; ESCHERICHIA COLI; GENETICS; ISOLATION AND PURIFICATION; METABOLISM; METHODOLOGY; MOLECULAR CLONING; MUTATION; PLASMID; PROTEIN SECONDARY STRUCTURE; SINDBIS VIRUS; SITE DIRECTED MUTAGENESIS; VIROLOGY;

ALPHAVIRUS INFECTIONS; CHROMATOGRAPHY, AFFINITY; CIRCULAR DICHROISM; CLONING, MOLECULAR; DETERGENTS; ENZYME ACTIVATION; ESCHERICHIA COLI; GUANOSINE TRIPHOSPHATE; HISTIDINE; METHYLTRANSFERASES; MUTAGENESIS, SITE-DIRECTED; MUTATION; NUCLEOTIDYLTRANSFERASES; OLIGOPEPTIDES; PLASMIDS; PROTEIN STRUCTURE, SECONDARY; RECOMBINANT PROTEINS; SINDBIS VIRUS; SUBSTRATE SPECIFICITY; TRANSFORMATION, BACTERIAL; VIRAL NONSTRUCTURAL PROTEINS;

ALPHAVIRUS; ESCHERICHIA COLI; SINDBIS VIRUS;

EID: 80051583576     PISSN: 10465928     EISSN: None     Source Type: Journal    
DOI: 10.1016/j.pep.2011.05.022     Document Type: Article

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