High-throughput illumina strand-specific RNA sequencing library preparation

Cold Spring Harbor Protocols

Volumn 6, Issue 8, 2011, Pages 940-949

  Zhong, Silin ^a,b   Joung, Je Gun ^a   Zheng, Yi ^a   Chen, Yun Ru ^a   Liu, Bao ^b   Shao, Ying ^c   Xiang, Jenny Z ^c   Fei, Zhangjun ^a,d   Giovannoni, James J ^a,d  

^a United States Department of Agriculture Agricultural Research Service and Boyce Thompson Institute for Plant Research   (United States)

^b NORTHEAST NORMAL UNIVERSITY   (China)

^c WEILL CORNELL MEDICAL COLLEGE   (United States)

^d U S DEPARTMENT OF AGRICULTURE   (United States)

Author keywords

[No Author keywords available]

Indexed keywords

COMPLEMENTARY DNA; PARATHION; RNA; SINGLE STRANDED RNA; TRANSCRIPTOME;

ARTICLE; CLINICAL PROTOCOL; DNA SYNTHESIS; EUKARYOTE; GENE EXPRESSION PROFILING; GENE LIBRARY; GENOMIC FRAGMENT; HIGH THROUGHPUT SEQUENCING; NONHUMAN; POLYMERASE CHAIN REACTION; RNA ISOLATION; RNA PURIFICATION; RNA REPAIR; RNA SEQUENCE; RNA STRAND; RNA STRUCTURE; RNA SYNTHESIS; SEQUENCE ANALYSIS; GENOMICS; METHODOLOGY;

GENE LIBRARY; GENOMICS; SEQUENCE ANALYSIS, RNA; TRANSCRIPTOME;

EID: 79961230772     PISSN: None     EISSN: 15596095     Source Type: Journal    
DOI: 10.1101/pdb.prot5652     Document Type: Article

References (4)

1. Craig DW, Pearson JV, Szelinger S, Sekar A, Redman M, Corneveaux JJ, Pawlowski TL, Laub T, Nunn G, Stephan DA, et al. 2008. Identification of genetic variants using bar-coded multiplexed sequencing. Nat Methods 5: 887-893.
2. Parkhomchuk D, Borodina T, Amstislavskiy V, Banaru M, Hallen L, Krobitsch S, Lehrach H, Soldatov A. 2009. Transcriptome analysis by strand-specific sequencing of complementary DNA. Nucleic Acids Res 37: e123. doi: 10.1093/nar/gkp596.
3. Vivancos AP, Güell M, Dohm JC, Serrano L, Himmelbauer H. 2010. Strand-specific deep sequencing of the transcriptome. Genome Res 20: 989-999.
4. Wang Z, Gerstein M, Snyder M. 2009. RNA-Seq: A revolutionary tool for transcriptomics. Nat Rev Genet 10: 57-63.