Comparison of commercial DNA extraction kits for isolation and purification of bacterial and eukaryotic DNA from PAH-contaminated soils

Canadian Journal of Microbiology

Volumn 57, Issue 8, 2011, Pages 623-628

  Mahmoudi, Nagissa ^a   Slater, Greg F ^a   Fulthorpe, Roberta R ^b  

^a MCMASTER UNIVERSITY   (Canada)

^b UNIVERSITY OF TORONTO   (Canada)

Author keywords

DGGE; DNA extraction; PAH biodegradation; PCR

Indexed keywords

16S RDNA; 18S RDNA; CONTAMINATED SOILS; DGGE; DNA EXTRACTION; DNA EXTRACTS; DNA ISOLATION; DNA YIELDS; EXTRACTION PROCESS; HIGH QUALITY; HUMIC ACID; INTRINSIC BIODEGRADATION; ISOLATION AND PURIFICATION; LIQUID-LIQUID EXTRACTION; MICROBIAL POPULATIONS; MOLECULAR ANALYSIS; MOLECULAR CHARACTERIZATION; MULTIPLE-BAND; ORGANIC CARBON CONTENTS; PCR; PCR PRODUCTS; SOIL DNA; SOIL DNA EXTRACTION; SOIL PROPERTY; SOIL TEXTURES; SOILS AND SEDIMENTS;

ANOXIC SEDIMENTS; BACTERIOLOGY; BIODEGRADATION; BIOLOGICAL MATERIALS; CONTAMINATION; DEGRADATION; DNA; ELECTROPHORESIS; ENZYME IMMOBILIZATION; HYDROCARBONS; LIQUIDS; ORGANIC ACIDS; ORGANIC CARBON; POLYCYCLIC AROMATIC HYDROCARBONS; SOIL POLLUTION; SOILS; SOLVENT EXTRACTION;

POLYMERASE CHAIN REACTION;

DNA; HUMIC ACID; ORGANIC CARBON; POLYCYCLIC AROMATIC HYDROCARBON;

AMPLIFICATION; BACTERIUM; BIODEGRADATION; COMPARATIVE STUDY; EUKARYOTE; EXTRACTION METHOD; HUMIC ACID; MICROBIAL COMMUNITY; MITOCHONDRIAL DNA; MOLECULAR ANALYSIS; ORGANIC CARBON; PAH; POLLUTANT REMOVAL; POLYMERASE CHAIN REACTION; PURIFICATION; SEDIMENT POLLUTION; SOIL POLLUTION; SOIL TEXTURE;

ARTICLE; BACTERIUM; BACTERIUM ISOLATION; CONTAMINATION; DENATURING GRADIENT GEL ELECTROPHORESIS; DNA EXTRACTION; EUKARYOTE; GENE AMPLIFICATION; INTERMETHOD COMPARISON; ISOLATION AND PURIFICATION; LIQUID LIQUID EXTRACTION; NONHUMAN; POLYMERASE CHAIN REACTION; PRIORITY JOURNAL; SOIL POLLUTION; SOIL TEXTURE;

BACTERIA; BIODEGRADATION, ENVIRONMENTAL; DENATURING GRADIENT GEL ELECTROPHORESIS; DNA; DNA, BACTERIAL; LIQUID-LIQUID EXTRACTION; POLYCYCLIC HYDROCARBONS, AROMATIC; POLYMERASE CHAIN REACTION; REAGENT KITS, DIAGNOSTIC; RNA, RIBOSOMAL, 16S; RNA, RIBOSOMAL, 18S; SOIL; SOIL MICROBIOLOGY; SOIL POLLUTANTS;

BACTERIA (MICROORGANISMS); EUKARYOTA;

EID: 79961230437     PISSN: 00084166     EISSN: 14803275     Source Type: Journal    
DOI: 10.1139/w11-049     Document Type: Article

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