Susceptibility of sperm chromatin to acid denaturation in situ: A study in endogenous FSH-deprived adult male bonnet monkeys (Macaca radiata)

Archives of Andrology

Volumn 40, Issue 1, 1998, Pages 29-41

  Aravindan, G R ^a,b   Moudgal, N R ^a  

^a INDIAN INSTITUTE OF SCIENCE   (India)

^b WASHINGTON STATE UNIVERSITY   (United States)

Author keywords

Acridine orange; Chromatin; Flow cytometry; FSH; Gonadotropin; Season; Spermatogenesis

Indexed keywords

ACRIDINE ORANGE; DOUBLE STRANDED DNA; FOLLITROPIN; GONADOTROPIN; IODINE 125; SINGLE STRANDED DNA;

ANIMAL CELL; ARTICLE; CHROMATIN; CONTROLLED STUDY; DNA DENATURATION; FLOW CYTOMETRY; HORMONE DEFICIENCY; IMMUNIZATION; MALE; MALE FERTILITY; MALE INFERTILITY; MONKEY; NONHUMAN; PRIORITY JOURNAL; SEASONAL VARIATION; SPERM; SPERMATOGENESIS;

EID: 79961201370     PISSN: 01485016     EISSN: None     Source Type: Journal    
DOI: 10.3109/01485019808987925     Document Type: Article

References (24)

1. Aravindan GR, Gopalakrishnan K, Ravindranath N, Moudgal NR (1993): Effect of altering endogenous gonadotrophin concentrations on the kinetics of testicular germ cell turnover in the bonnet monkey (Macaca radiata). J Endocrinol 137:485-495.
2. Aravindan GR, Moudgal NR (1991): FSH deprivation affects spermiogenesis and sperm quality in the adult non-human primate. In: Immunology: Perspectives in Reproduction and Infections. Gupta SK (Ed). New Delhi: Oxford-IBH, pp 227-236.
3. Aravindan GR, Ravindranath N, Krishnamurthy H, Moudgal NR (1991): FSH deprivation in bonnet monkeys (Macaca radiata) affects ability of sperm DNA to bind acridine orange and undergo decondensation in vitro. Biol Reprod 44 (suppl 1):181-Abst. 514.
4. Aravindan GR, Ravindranath N, Moudgal NR (1991): Use of DNA flow cytometry in assessing gonadotropin regulation of spermatogenesis in monkeys. In: Perspectives in Primate Reproductive Biology. Moudgal NR, Yoshinaga K, Rao AJ, Adiga PR (Eds). New Delhi: Wiley Eastern, pp 189-199.
5. Ballachey BE, Saacke RG, Evenson DP (1987): Heterogeneity of sperm nuclear chromatin structure and its relationship to fertility of bulls. Biol Reprod 36:915-925.
6. Darzynkiewicz Z (1979): Acridine orange as a molecular probe in studies of nucleic acids in situ. In: Flow Cytometry and Sorting. Melamed MR, Mullaney PF, Mendelsohn ML (Eds). New York: Wiley, pp 285-316.
7. Evenson DP, Melamed MR (1983): Rapid analysis of normal and abnormal cell types in human semen and testis biopsies by flow cytometry. J Histochem Cytochem 31:248-253.
8. 3H actinomycin D binding to nuclear chromatin of morphologically abnormal spermatozoa. J Reprod Fertil 26:25-38.
9. Kapuscinski J, Darzynkiewicz Z (1984): Denaturation of nucleic acids induced by intercalating agents: biochemical and biophysical properties of acridine orange-DNA complexes. J Biomol Struct 1:1485-1499.
10. Kapuscinski J, Darzynkiewicz Z, Melamed MR (1982): Luminescence of solid complexes of acridine orange with RNA. Cytometry 2:201-211.
11. Medhamurthy R, Suresh R, Sunil Paul S, Moudgal NR (1994): Correlation of seasonal changes in sperm output with endocrinological changes in the adult male bonnet monkey, Macaca radiata. J Biosci 19:67-74.
12. Melamed MR, Darzynkiewicz Z (1981): Acridine orange as a quantitative cytochemical probe for flow cytometry. In: Histochemistry: The Widening Horizons. Stoward PJ, Polak JM (Eds). New York: Wiley, pp 237-261.
13. Monesi V (1965): Synthetic activities during spermatogenesis in the mouse: RNA and protein. Exp Cell Res 39:197-224.
14. Moudgal NR, Aravindan GR (1993): Induction of infertility in the male by blocking follicle-stimulating hormone action. In: Immunology of Reproduction. Naz RK (Ed). Boca Raton, FL: CRC Press, pp 251-277.
15. Moudgal NR, Ravindranath N, Murthy GS, Dighe RR, Aravindan GR, Martin P (1992): Long term contraceptive efficacy of vaccine of ovine follicle-stimulating hormone in male monkeys (Macaca radiata). J Reprod Fertil 96:91-102.
16. Murty GSRC, Sheela Rani CS, Moudgal NR, Prasad MRN (1979): Effect of passive immunization with specific antiserum to FSH on the spermatogenic process and fertility of adult male bonnet monkeys (Macaca radiata). J Reprod Fertil (Suppl) 26:147-363.
17. Peluso JJ, Luciano AA, Nulsen JC (1992): The relationship between alterations in spermatozoal deoxyribonucleic acid, heparin binding sites, and semen quality. Fertil Steril 57:665-670.
18. Raj HGM, Kotagi SG, Letellier R, Nath N, Raj SG (1991): Active immunization with gonadotropins in the crab eating monkey (Macaca fascicularis): evaluation for male contraception. In: Perspectives in Primate Reproductive Biology. Moudgal NR, Yoshinaga K, Rao AJ, Adiga PR (Eds). New Delhi: Wiley Eastern, pp 307-316.
19. Ravindranath N, Moudgal NR (1987): Use of Tamoxifen, an antioestrogen, in establishing a need for oestrogen in early pregnancy in the bonnet monkey (Macaca radiata). J Reprod Fertil 81:327-336.
20. Ravindranath N, Sheela Rani CS, Martin F, Moudgal NR (1989): Effect of FSH deprivation at specific times on follicular maturation in the bonnet monkey (Macaca radiata). J Reprod Fertil 87:231-341.
21. Ringertz NR, Gledhill BL, Darzynkiewicz Z (1970): Changes in deoxyribonucleoprotein during spermiogenesis in the bull: sensitivity of DNA to heat denaturation. Exp Cell Res 62:204-218.
22. Srinath BR (1979): Husbandry and breeding of bonnet monkeys (Macaca radiata). In: Non-human Primate Models for Study of Human Reproduction. Anand Kumar TC (Ed). Basel: Karger, pp 17-22.
23. Srivastava A, Das RP (1992): Sperm production and fertility of bonnet monkeys (Macaca radiata) following immunization with ovine follicle stimulating hormone. Indian J Exp Biol 30:574-577.
24. Tejada RI, Mitchell JC, Norman A, Marik JJ, Friedman S (1984): A test for the practical evaluation of male fertility by acridine orange (AO) fluorescence. Fertil Steril 42:87-91.