Detection of murine norovirus-1 by using TAT peptide-delivered molecular beacons

Applied and Environmental Microbiology

Volumn 77, Issue 15, 2011, Pages 5517-5520

  Ganguli, Payal S ^a   Chen, Wilfred ^b   Yates, Marylynn V ^a  

^a UNIVERSITY OF CALIFORNIA   (United States)

^b University of Delaware   (United States)

Author keywords

[No Author keywords available]

Indexed keywords

MOLECULAR BEACON; PLAQUE ASSAY; VIRAL DETECTION;

PEPTIDES; VIRUSES;

COMPUTER VIRUSES;

FLUORESCENT DYE; TRANSACTIVATOR PROTEIN; VIRUS RNA;

BIOASSAY; CELL ORGANELLE; DETECTION METHOD; INFECTIVITY; MOLECULAR ANALYSIS; PEPTIDE; VIRUS;

ANIMAL; ARTICLE; GENETICS; ISOLATION AND PURIFICATION; MOUSE; NOROVIRUS; OLIGONUCLEOTIDE PROBE; TUMOR CELL LINE;

ANIMALS; CELL LINE, TUMOR; FLUORESCENT DYES; GENE PRODUCTS, TAT; MICE; NOROVIRUS; OLIGONUCLEOTIDE PROBES; RNA, VIRAL;

MURINE NOROVIRUS 1; NOROVIRUS;

EID: 79961053443     PISSN: 00992240     EISSN: 10985336     Source Type: Journal    
DOI: 10.1128/AEM.03048-10     Document Type: Article

References (20)

1. Bae, J., and K. J. Schwab. 2008. Evaluation of murine norovirus, feline calicivirus, poliovirus, and MS2 as surrogates for human norovirus in a model of viral persistence in surface water and groundwater. Appl. Environ. Microbiol. 74:477-484.
2. Becker-Hapak, M., S. S. McAllister, and S. F. Dowdy. 2001. TAT-mediated protein transduction into mammalian cells. Methods 24:247-256.
3. Bidawid, S., N. Malik, O. Adegbunrin, S. A. Sattar, and J. M. Farber. 2003. A feline kidney cell line-based plaque assay for feline calicivirus, a surrogate for Norwalk virus. J. Virol. Methods 107:163-167.
4. Duizer, E., et al. 2004. Laboratory efforts to cultivate noroviruses. J. Gen. Virol. 85:79-87.
5. Fang, X., et al. 2002. Molecular beacons: fluorogenic probes for living cell study. Cell Biochem. Biophys. 37:71-81.
6. Fankhauser, R. L., J. S. Noel, S. S. Monroe, T. Ando, and R. I. Glass. 1998. Molecular epidemiology of "Norwalk-like viruses" in outbreaks of gastroenteritis in the United States. J. Infect. Dis. 178:1571-1578.
7. Lindell, A. T., L. Grillner, L. Svensson, and B. Z. Wirgart. 2005. Molecular epidemiology of norovirus infections in Stockholm, Sweden, during the years 2000 to 2003: association of the GGIIb genetic cluster with infection in children. J. Clin. Microbiol. 43:1086-1092.
8. Lopman, B. A., D. W. Brown, and M. Koopmans. 2002. Human caliciviruses in Europe. J. Clin. Virol. 24:137-160.
9. Marras, S. A., F. R. Kramer, and S. Tyagi. 1999. Multiplex detection of single-nucleotide variations using molecular beacons. Genet. Anal. 14:151-156.
10. Mead, P. S., et al. 1999. Food-related illness and death in the United States. Emerg. Infect. Dis. 5:607-625.
11. Nitin, N., P. J. Santangelo, G. Kim, S. Nie, and G. Bao. 2004. Peptide-linked molecular beacons for efficient delivery and rapid mRNA detection in living cells. Nucleic Acids Res. 32:e58.
12. Schwab, K. J., R. De Leon, and M. D. Sobsey. 1995. Concentration and purification of beef extract mock eluates from water samples for the detection of enteroviruses, hepatitis A virus, and Norwalk virus by reverse transcription-PCR. Appl. Environ. Microbiol. 61:531-537.
13. Straub, T. M., et al. 2007. In vitro cell culture infectivity assay for human noroviruses. Emerg. Infect. Dis. 13:396-403.
14. Tsourkas, A., M. A. Behlke, and G. Bao. 2002. Hybridization of 2'-O-methyl and 2'-deoxy molecular beacons to RNA and DNA targets. Nucleic Acids Res. 30:5168-5174.
15. Tyagi, S., and F. R. Kramer. 1996. Molecular beacons: probes that fluoresce upon hybridization. Nat. Biotechnol. 14:303-308.
16. Wang, A., A. M. Salazar, M. V. Yates, A. Mulchandani, and W. Chen. 2005. Visualization and detection of infectious coxsackievirus replication using a combined cell culture-molecular beacon assay. Appl. Environ. Microbiol. 71:8397-8401.
17. Wobus, C. E., et al. 2004. Replication of norovirus in cell culture reveals a tropism for dendritic cells and macrophages. PLoS Biol. 2:e432.
18. Wobus, C. E., L. B. Thackray, and H. W. Virgin IV. 2006. Murine norovirus: a model system to study norovirus biology and pathogenesis. J. Virol. 80: 5104-5112.
19. Yeh, H. Y., M. V. Yates, W. Chen, and A. Mulchandani. 2009. Real-time molecular methods to detect infectious viruses. Semin. Cell Dev. Biol. 20: 49-54.
20. Yeh, H. Y., M. V. Yates, A. Mulchandani, and W. Chen. 2008. Visualizing the dynamics of viral replication in living cells via Tat peptide delivery of nuclease-resistant molecular beacons. Proc. Natl. Acad. Sci. U. S. A. 105: 17522-17525.