메뉴 건너뛰기




Volumn 75, Issue , 2011, Pages 231-250

Genetic Techniques in Trypanosoma cruzi

Author keywords

Functional analysis; Genetic manipulation; Transfection; Transgenic; Trypanosoma cruzi

Indexed keywords

DNA; PEROXIDE; RNA;

EID: 79961011258     PISSN: 0065308X     EISSN: None     Source Type: Book Series    
DOI: 10.1016/B978-0-12-385863-4.00011-3     Document Type: Chapter
Times cited : (24)

References (68)
  • 1
    • 47949091926 scopus 로고    scopus 로고
    • Single-locus targeting constructs for reliable RNAi and transgene expression in Trypanosoma brucei
    • Alsford S., Horn D. Single-locus targeting constructs for reliable RNAi and transgene expression in Trypanosoma brucei. Mol. Biochem. Parasitol. 2008, 161:76-79.
    • (2008) Mol. Biochem. Parasitol. , vol.161 , pp. 76-79
    • Alsford, S.1    Horn, D.2
  • 2
    • 33748195107 scopus 로고    scopus 로고
    • A rapid, reversible, and tunable method to regulate protein function in living cells using synthetic small molecules
    • Banaszynski L.A., Chen L.C., Maynard-Smith L.A., Ooi A.G., Wandless T.J. A rapid, reversible, and tunable method to regulate protein function in living cells using synthetic small molecules. Cell 2006, 126:995-1004.
    • (2006) Cell , vol.126 , pp. 995-1004
    • Banaszynski, L.A.1    Chen, L.C.2    Maynard-Smith, L.A.3    Ooi, A.G.4    Wandless, T.J.5
  • 4
    • 0024345942 scopus 로고
    • Expression of a bacterial gene in a trypanosomatid protozoan
    • Bellofatto V., Cross G.A. Expression of a bacterial gene in a trypanosomatid protozoan. Science 1989, 244:1167-1169.
    • (1989) Science , vol.244 , pp. 1167-1169
    • Bellofatto, V.1    Cross, G.A.2
  • 5
    • 0027176807 scopus 로고
    • DNA-mediated transformation of bloodstream-form Trypanosoma brucei
    • Carruthers V.B., van der Ploeg L.H., Cross G.A. DNA-mediated transformation of bloodstream-form Trypanosoma brucei. Nucleic Acids Res. 1993, 21:2537-2538.
    • (1993) Nucleic Acids Res. , vol.21 , pp. 2537-2538
    • Carruthers, V.B.1    van der Ploeg, L.H.2    Cross, G.A.3
  • 7
    • 0027158311 scopus 로고
    • Deletion of an immunodominant Trypanosoma cruzi surface glycoprotein disrupts flagellum-cell adhesion
    • Cooper R., de Jesus A.R., Cross G.A. Deletion of an immunodominant Trypanosoma cruzi surface glycoprotein disrupts flagellum-cell adhesion. J. Cell Biol. 1993, 122:149-156.
    • (1993) J. Cell Biol. , vol.122 , pp. 149-156
    • Cooper, R.1    de Jesus, A.R.2    Cross, G.A.3
  • 8
    • 0025049856 scopus 로고
    • Gene replacement in parasitic protozoa
    • Cruz A., Beverley S.M. Gene replacement in parasitic protozoa. Nature 1990, 348:171-173.
    • (1990) Nature , vol.348 , pp. 171-173
    • Cruz, A.1    Beverley, S.M.2
  • 10
    • 0347418186 scopus 로고    scopus 로고
    • Tests of cytoplasmic RNA interference (RNAi) and construction of a tetracycline-inducible T7 promoter system in Trypanosoma cruzi
    • DaRocha W.D., Otsu K., Teixeira S.M., Donelson J.E. Tests of cytoplasmic RNA interference (RNAi) and construction of a tetracycline-inducible T7 promoter system in Trypanosoma cruzi. Mol. Biochem. Parasitol. 2004, 133:175-186.
    • (2004) Mol. Biochem. Parasitol. , vol.133 , pp. 175-186
    • DaRocha, W.D.1    Otsu, K.2    Teixeira, S.M.3    Donelson, J.E.4
  • 11
    • 0442329282 scopus 로고    scopus 로고
    • Expression of exogenous genes in Trypanosoma cruzi: improving vectors and electroporation protocols
    • DaRocha W.D., Silva R.A., Bartholomeu D.C., Pires S.F., Freitas J.M., Macedo A.M., et al. Expression of exogenous genes in Trypanosoma cruzi: improving vectors and electroporation protocols. Parasitol. Res. 2004, 92:113-120.
    • (2004) Parasitol. Res. , vol.92 , pp. 113-120
    • DaRocha, W.D.1    Silva, R.A.2    Bartholomeu, D.C.3    Pires, S.F.4    Freitas, J.M.5    Macedo, A.M.6
  • 12
    • 2442597534 scopus 로고    scopus 로고
    • TbAGO1, an argonaute protein required for RNA interference, is involved in mitosis and chromosome segregation in Trypanosoma brucei
    • Durand-Dubief M., Bastin P. TbAGO1, an argonaute protein required for RNA interference, is involved in mitosis and chromosome segregation in Trypanosoma brucei. BMC Biol. 2003, 1:2.
    • (2003) BMC Biol. , vol.1 , pp. 2
    • Durand-Dubief, M.1    Bastin, P.2
  • 13
    • 0027509267 scopus 로고
    • Stable transformation of Trypanosoma cruzi: inactivation of the PUB12.5 polyubiquitin gene by targeted gene disruption
    • Hariharan S., Ajioka J., Swindle J. Stable transformation of Trypanosoma cruzi: inactivation of the PUB12.5 polyubiquitin gene by targeted gene disruption. Mol. Biochem. Parasitol. 1993, 57:15-30.
    • (1993) Mol. Biochem. Parasitol. , vol.57 , pp. 15-30
    • Hariharan, S.1    Ajioka, J.2    Swindle, J.3
  • 15
    • 54949087530 scopus 로고    scopus 로고
    • Insights into the redox biology of Trypanosoma cruzi: trypanothione metabolism and oxidant detoxification
    • Irigoín F., Cibils L., Comini M.A., Wilkinson S.R., Flohé L., Radi R. Insights into the redox biology of Trypanosoma cruzi: trypanothione metabolism and oxidant detoxification. Free Radic. Biol. Med. 2008, 45:733-742.
    • (2008) Free Radic. Biol. Med. , vol.45 , pp. 733-742
    • Irigoín, F.1    Cibils, L.2    Comini, M.A.3    Wilkinson, S.R.4    Flohé, L.5    Radi, R.6
  • 16
    • 0025122367 scopus 로고
    • Stable transfection of the human parasite Leishmania major delineates a 30-kilobase region sufficient for extrachromosomal replication and expression
    • Kapler G.M., Coburn C.M., Beverley S.M. Stable transfection of the human parasite Leishmania major delineates a 30-kilobase region sufficient for extrachromosomal replication and expression. Mol. Cell. Biol. 1990, 10:1084-1094.
    • (1990) Mol. Cell. Biol. , vol.10 , pp. 1084-1094
    • Kapler, G.M.1    Coburn, C.M.2    Beverley, S.M.3
  • 17
    • 0026673614 scopus 로고
    • A shuttle vector which facilitates the expression of transfected genes in Trypanosoma cruzi and Leishmania
    • Kelly J.M., Ward H.M., Miles M.A., Kendall G. A shuttle vector which facilitates the expression of transfected genes in Trypanosoma cruzi and Leishmania. Nucleic Acids Res. 1992, 20:3963-3969.
    • (1992) Nucleic Acids Res. , vol.20 , pp. 3963-3969
    • Kelly, J.M.1    Ward, H.M.2    Miles, M.A.3    Kendall, G.4
  • 18
    • 0027494350 scopus 로고
    • Phenotype of recombinant Leishmania donovani and Trypanosoma cruzi which overexpress trypanothione reductase: sensitivity towards agents that are thought to induce oxidative stress
    • Kelly J.M., Taylor M.C., Smith K., Hunter K.J., Fairlamb A.H. Phenotype of recombinant Leishmania donovani and Trypanosoma cruzi which overexpress trypanothione reductase: sensitivity towards agents that are thought to induce oxidative stress. Eur. J. Biochem. 1993, 218:29-37.
    • (1993) Eur. J. Biochem. , vol.218 , pp. 29-37
    • Kelly, J.M.1    Taylor, M.C.2    Smith, K.3    Hunter, K.J.4    Fairlamb, A.H.5
  • 19
    • 0027998830 scopus 로고
    • An approach to functional complementation by introduction of large DNA fragments into Trypanosoma cruzi and Leishmania donovani using a cosmid shuttle vector
    • Kelly J.M., Das P., Tomas A.M. An approach to functional complementation by introduction of large DNA fragments into Trypanosoma cruzi and Leishmania donovani using a cosmid shuttle vector. Mol. Biochem. Parasitol. 1994, 65:51-62.
    • (1994) Mol. Biochem. Parasitol. , vol.65 , pp. 51-62
    • Kelly, J.M.1    Das, P.2    Tomas, A.M.3
  • 20
    • 0029188769 scopus 로고
    • Transfection of the African and American trypanosomes
    • Humana Press, New Jersey, J.A. Nickoloff (Ed.) Electroporation Protocols for Microorganisms
    • Kelly J.M., Taylor M.C., Rudenko G., Blundell P.A. Transfection of the African and American trypanosomes. Methods in Molecular Biology 1995, Vol. 47:349-359. Humana Press, New Jersey. J.A. Nickoloff (Ed.).
    • (1995) Methods in Molecular Biology , vol.47 , pp. 349-359
    • Kelly, J.M.1    Taylor, M.C.2    Rudenko, G.3    Blundell, P.A.4
  • 21
    • 0025182371 scopus 로고
    • Trypanosoma cruzi glycosomal glyceraldehyde-3-phosphate dehydrogenase does not conform to the " hotspot" topogenic signal model
    • Kendall G., Wilderspin A.F., Ashall F., Miles M.A., Kelly J.M. Trypanosoma cruzi glycosomal glyceraldehyde-3-phosphate dehydrogenase does not conform to the " hotspot" topogenic signal model. EMBO J. 1990, 9:2751-2758.
    • (1990) EMBO J. , vol.9 , pp. 2751-2758
    • Kendall, G.1    Wilderspin, A.F.2    Ashall, F.3    Miles, M.A.4    Kelly, J.M.5
  • 23
    • 0024331981 scopus 로고
    • Transfection of Leishmania enriettii and expression of chloramphenicol acetyltransferase gene
    • Laban A., Wirth D.F. Transfection of Leishmania enriettii and expression of chloramphenicol acetyltransferase gene. Proc. Natl. Acad. Sci. USA 1989, 86:9119-9123.
    • (1989) Proc. Natl. Acad. Sci. USA , vol.86 , pp. 9119-9123
    • Laban, A.1    Wirth, D.F.2
  • 25
    • 63249133197 scopus 로고    scopus 로고
    • Gene expression modulation is associated with gene amplification, supernumerary chromosomes and chromosome loss in antimony-resistant Leishmania infantum
    • Leprohon P., Legare D., Raymond F., Madore E., Hardiman G., Corbeil J., et al. Gene expression modulation is associated with gene amplification, supernumerary chromosomes and chromosome loss in antimony-resistant Leishmania infantum. Nucleic Acids Res. 2009, 37:1387-1399.
    • (2009) Nucleic Acids Res. , vol.37 , pp. 1387-1399
    • Leprohon, P.1    Legare, D.2    Raymond, F.3    Madore, E.4    Hardiman, G.5    Corbeil, J.6
  • 26
    • 0025966025 scopus 로고
    • Expression of an exogenous gene in Trypanosoma cruzi epimastigotes
    • Lu H.Y., Buck G.A. Expression of an exogenous gene in Trypanosoma cruzi epimastigotes. Mol. Biochem. Parasitol. 1991, 44:109-114.
    • (1991) Mol. Biochem. Parasitol. , vol.44 , pp. 109-114
    • Lu, H.Y.1    Buck, G.A.2
  • 27
  • 28
    • 0028358229 scopus 로고
    • Trypanosoma cruzi ribosomal DNA: mapping of a putative distal promoter
    • Martínez-Calvillo S., Hernández R. Trypanosoma cruzi ribosomal DNA: mapping of a putative distal promoter. Gene 1994, 142:243-247.
    • (1994) Gene , vol.142 , pp. 243-247
    • Martínez-Calvillo, S.1    Hernández, R.2
  • 29
    • 0343416375 scopus 로고    scopus 로고
    • PRIBOTEX expression vector: a pTEX derivative for a rapid selection of Trypanosoma cruzi transfectants
    • Martínez-Calvillo S., López I., Hernández R. pRIBOTEX expression vector: a pTEX derivative for a rapid selection of Trypanosoma cruzi transfectants. Gene 1997, 199:71-76.
    • (1997) Gene , vol.199 , pp. 71-76
    • Martínez-Calvillo, S.1    López, I.2    Hernández, R.3
  • 30
    • 0032956467 scopus 로고    scopus 로고
    • Optimisation of conditions for growth of wild-type and genetically transformed Trypanosoma cruzi on agarose plates
    • Mondragon A., Wilkinson S.R., Taylor M.C., Kelly J.M. Optimisation of conditions for growth of wild-type and genetically transformed Trypanosoma cruzi on agarose plates. Parasitology 1999, 118:461-467.
    • (1999) Parasitology , vol.118 , pp. 461-467
    • Mondragon, A.1    Wilkinson, S.R.2    Taylor, M.C.3    Kelly, J.M.4
  • 31
    • 0028041048 scopus 로고
    • Functional complementation of glycoprotein 72 in a Trypanosoma cruzi glycoprotein 72 null mutant
    • Nozaki T., Cross G.A. Functional complementation of glycoprotein 72 in a Trypanosoma cruzi glycoprotein 72 null mutant. Mol. Biochem. Parasitol. 1994, 67:91-102.
    • (1994) Mol. Biochem. Parasitol. , vol.67 , pp. 91-102
    • Nozaki, T.1    Cross, G.A.2
  • 32
    • 12744273368 scopus 로고    scopus 로고
    • Functional mapping of a trypanosome centromere by chromosome fragmentation identifies a 16kb GC-rich transcriptional " strand-switch" domain as a major feature
    • Obado S.O., Taylor M.C., Wilkinson S.R., Bromley E.V., Kelly J.M. Functional mapping of a trypanosome centromere by chromosome fragmentation identifies a 16kb GC-rich transcriptional " strand-switch" domain as a major feature. Genome Res. 2005, 15:36-43.
    • (2005) Genome Res. , vol.15 , pp. 36-43
    • Obado, S.O.1    Taylor, M.C.2    Wilkinson, S.R.3    Bromley, E.V.4    Kelly, J.M.5
  • 33
    • 34250179515 scopus 로고    scopus 로고
    • Repetitive DNA is associated with centromeric domains in Trypanosoma brucei but not Trypanosoma cruzi
    • Obado S.O., Bot C., Nilsson D., Andersson B., Kelly J.M. Repetitive DNA is associated with centromeric domains in Trypanosoma brucei but not Trypanosoma cruzi. Genome Biol. 2007, 8:R37.
    • (2007) Genome Biol. , vol.8
    • Obado, S.O.1    Bot, C.2    Nilsson, D.3    Andersson, B.4    Kelly, J.M.5
  • 35
    • 0027392952 scopus 로고
    • Interruption of a Trypanosoma cruzi gene encoding a protein containing 14-amino acid repeats by targeted insertion of the neomycin phosphotransferase gene
    • Otsu K., Donelson J.E., Kirchhoff L.V. Interruption of a Trypanosoma cruzi gene encoding a protein containing 14-amino acid repeats by targeted insertion of the neomycin phosphotransferase gene. Mol. Biochem. Parasitol. 1993, 57:317-330.
    • (1993) Mol. Biochem. Parasitol. , vol.57 , pp. 317-330
    • Otsu, K.1    Donelson, J.E.2    Kirchhoff, L.V.3
  • 37
    • 0028070305 scopus 로고
    • Autonomous replication of bacterial DNA plasmid oligomers in Leishmania
    • Papadopoulou B., Roy G., Ouellette M. Autonomous replication of bacterial DNA plasmid oligomers in Leishmania. Mol. Biochem. Parasitol. 1994, 65:39-49.
    • (1994) Mol. Biochem. Parasitol. , vol.65 , pp. 39-49
    • Papadopoulou, B.1    Roy, G.2    Ouellette, M.3
  • 38
    • 0027230120 scopus 로고
    • Autonomously replicating single-copy episomes in Trypanosoma brucei show unusual stability
    • Patnaik P.K., Kulkarni S.K., Cross G.A. Autonomously replicating single-copy episomes in Trypanosoma brucei show unusual stability. EMBO J. 1993, 12:2529-2538.
    • (1993) EMBO J. , vol.12 , pp. 2529-2538
    • Patnaik, P.K.1    Kulkarni, S.K.2    Cross, G.A.3
  • 39
    • 70449560425 scopus 로고    scopus 로고
    • Distinct and overlapping roles for two Dicer-like proteins in the RNA interference pathways of the ancient eukaryote Trypanosoma brucei
    • Patrick K.L., Shi N.G., Kolev K., Ersfeld C., Tschudi C., Ullu E. Distinct and overlapping roles for two Dicer-like proteins in the RNA interference pathways of the ancient eukaryote Trypanosoma brucei. Proc. Natl. Acad. Sci. USA 2009, 106:17933-17938.
    • (2009) Proc. Natl. Acad. Sci. USA , vol.106 , pp. 17933-17938
    • Patrick, K.L.1    Shi, N.G.2    Kolev, K.3    Ersfeld, C.4    Tschudi, C.5    Ullu, E.6
  • 40
    • 34347339518 scopus 로고    scopus 로고
    • Comparative genomic analysis of three Leishmania species that cause diverse human disease
    • Peacock C.S., Seeger K., Harris D., Murphy L., Ruiz J.C., Quail M.A., et al. Comparative genomic analysis of three Leishmania species that cause diverse human disease. Nat. Genet. 2007, 39:839-847.
    • (2007) Nat. Genet. , vol.39 , pp. 839-847
    • Peacock, C.S.1    Seeger, K.2    Harris, D.3    Murphy, L.4    Ruiz, J.C.5    Quail, M.A.6
  • 41
    • 34247107954 scopus 로고    scopus 로고
    • Mitochondrial superoxide radicals mediate programmed cell death in Trypanosoma cruzi: cytoprotective action of mitochondrial Fe-superoxide dismutase overexpression
    • Piacenza L., Irigoín F., Alvarez M.N., Peluffo G., Taylor M.C., Kelly J.M., et al. Mitochondrial superoxide radicals mediate programmed cell death in Trypanosoma cruzi: cytoprotective action of mitochondrial Fe-superoxide dismutase overexpression. Biochem. J. 2007, 403:323-334.
    • (2007) Biochem. J. , vol.403 , pp. 323-334
    • Piacenza, L.1    Irigoín, F.2    Alvarez, M.N.3    Peluffo, G.4    Taylor, M.C.5    Kelly, J.M.6
  • 42
    • 40449096623 scopus 로고    scopus 로고
    • Peroxiredoxins play a major role in protecting Trypanosoma cruzi against macrophage- and endogenously derived peroxynitrite
    • Piacenza L., Peluffo G., Alvarez M.N., Kelly J.M., Wilkinson S.R., Radi R. Peroxiredoxins play a major role in protecting Trypanosoma cruzi against macrophage- and endogenously derived peroxynitrite. Biochem. J. 2008, 410:359-368.
    • (2008) Biochem. J. , vol.410 , pp. 359-368
    • Piacenza, L.1    Peluffo, G.2    Alvarez, M.N.3    Kelly, J.M.4    Wilkinson, S.R.5    Radi, R.6
  • 43
    • 68049131348 scopus 로고    scopus 로고
    • Fighting the oxidative assault: the Trypanosoma cruzi journey to infection
    • Piacenza L., Alvarez M.N., Peluffo G., Radi R. Fighting the oxidative assault: the Trypanosoma cruzi journey to infection. Curr. Opin. Microbiol. 2009, 12(4):415-421.
    • (2009) Curr. Opin. Microbiol. , vol.12 , Issue.4 , pp. 415-421
    • Piacenza, L.1    Alvarez, M.N.2    Peluffo, G.3    Radi, R.4
  • 45
    • 37549058462 scopus 로고    scopus 로고
    • Peroxiredoxins from Trypanosoma cruzi: virulence factors and drug targets for treatment of Chagas disease?
    • Piñeyro M.D., Parodi-Talice A., Arcari T., Robello C. Peroxiredoxins from Trypanosoma cruzi: virulence factors and drug targets for treatment of Chagas disease?. Gene 2008, 408:45-50.
    • (2008) Gene , vol.408 , pp. 45-50
    • Piñeyro, M.D.1    Parodi-Talice, A.2    Arcari, T.3    Robello, C.4
  • 46
    • 0025291294 scopus 로고
    • Procyclic acidic repetitive protein (PARP) genes located in an unusually small alpha-amanitin-resistant transcription unit: PARP promoter activity assayed by transient DNA transfection of Trypanosoma brucei
    • Rudenko G., Le Blancq S., Smith J., Lee M.G., Rattray A., Van der Ploeg L.H. Procyclic acidic repetitive protein (PARP) genes located in an unusually small alpha-amanitin-resistant transcription unit: PARP promoter activity assayed by transient DNA transfection of Trypanosoma brucei. Mol. Cell. Biol. 1990, 10:3492-3504.
    • (1990) Mol. Cell. Biol. , vol.10 , pp. 3492-3504
    • Rudenko, G.1    Le Blancq, S.2    Smith, J.3    Lee, M.G.4    Rattray, A.5    Van der Ploeg, L.H.6
  • 47
    • 26044437984 scopus 로고    scopus 로고
    • Functional complementation of RNA interference mutants in trypanosomes
    • Rusconi F., Durand-Dubief M., Bastin P. Functional complementation of RNA interference mutants in trypanosomes. BMC Biotechnol. 2005, 5:6.
    • (2005) BMC Biotechnol. , vol.5 , pp. 6
    • Rusconi, F.1    Durand-Dubief, M.2    Bastin, P.3
  • 48
    • 0346363759 scopus 로고    scopus 로고
    • Argonaute protein in the early divergent eukaryote Trypanosoma brucei: control of small interfering RNA accumulation and retroposon transcript abundance
    • Shi H., Djikeng A., Tschudi C., Ullu E. Argonaute protein in the early divergent eukaryote Trypanosoma brucei: control of small interfering RNA accumulation and retroposon transcript abundance. Mol. Cell. Biol. 2004, 24:420-427.
    • (2004) Mol. Cell. Biol. , vol.24 , pp. 420-427
    • Shi, H.1    Djikeng, A.2    Tschudi, C.3    Ullu, E.4
  • 49
    • 33845270165 scopus 로고    scopus 로고
    • An unusual Dicer-like1 protein fuels the RNA interference pathway in Trypanosoma brucei
    • Shi H., Tschudi C., Ullu E. An unusual Dicer-like1 protein fuels the RNA interference pathway in Trypanosoma brucei. RNA 2006, 12:2063-2072.
    • (2006) RNA , vol.12 , pp. 2063-2072
    • Shi, H.1    Tschudi, C.2    Ullu, E.3
  • 50
    • 33747332260 scopus 로고    scopus 로고
    • A stable tetracycline-regulated gene expression system for Trypanosoma cruzi
    • Taylor M.C., Kelly J.M. A stable tetracycline-regulated gene expression system for Trypanosoma cruzi. BMC Biotechnol. 2006, 6:32.
    • (2006) BMC Biotechnol. , vol.6 , pp. 32
    • Taylor, M.C.1    Kelly, J.M.2
  • 51
    • 0025010981 scopus 로고
    • Targeted insertion of the neomycin phosphotransferase gene into the tubulin gene cluster ofTrypanosoma brucei
    • ten Asbroek A.L., Ouellette M., Borst P. Targeted insertion of the neomycin phosphotransferase gene into the tubulin gene cluster ofTrypanosoma brucei. Nature 1990, 348:174-175.
    • (1990) Nature , vol.348 , pp. 174-175
    • ten Asbroek, A.L.1    Ouellette, M.2    Borst, P.3
  • 53
    • 0029040980 scopus 로고
    • A rapid method for protein localization in trypanosomes
    • Tibbetts R.S., Klein K.G., Engman D.M. A rapid method for protein localization in trypanosomes. Exp. Parasitol. 1995, 80:572-574.
    • (1995) Exp. Parasitol. , vol.80 , pp. 572-574
    • Tibbetts, R.S.1    Klein, K.G.2    Engman, D.M.3
  • 54
    • 0031047603 scopus 로고    scopus 로고
    • Overexpression of cruzipain, the major cysteine proteinase of Trypanosoma cruzi is associated with enhanced metacyclogenesis
    • Tomas A.M., Miles M.A., Kelly J.M. Overexpression of cruzipain, the major cysteine proteinase of Trypanosoma cruzi is associated with enhanced metacyclogenesis. Eur. J. Biochem. 1997, 244:596-603.
    • (1997) Eur. J. Biochem. , vol.244 , pp. 596-603
    • Tomas, A.M.1    Miles, M.A.2    Kelly, J.M.3
  • 55
    • 49049118494 scopus 로고    scopus 로고
    • Modulation of gene expression in drug resistant Leishmania is associated with gene amplification, gene deletion and chromosome aneuploidy
    • Ubeda J.M., Legare D., Raymond F., Ouameur A.A., Boisvert S., Rigault P., et al. Modulation of gene expression in drug resistant Leishmania is associated with gene amplification, gene deletion and chromosome aneuploidy. Genome Biol. 2008, 9:R115.
    • (2008) Genome Biol. , vol.9
    • Ubeda, J.M.1    Legare, D.2    Raymond, F.3    Ouameur, A.A.4    Boisvert, S.5    Rigault, P.6
  • 57
    • 0032760732 scopus 로고    scopus 로고
    • Functional analysis of the intergenic regions of TcP2beta gene loci allowed the construction of an improved Trypanosoma cruzi expression vector
    • Vazquez M.P., Levin M.J. Functional analysis of the intergenic regions of TcP2beta gene loci allowed the construction of an improved Trypanosoma cruzi expression vector. Gene 1999, 239:217-225.
    • (1999) Gene , vol.239 , pp. 217-225
    • Vazquez, M.P.1    Levin, M.J.2
  • 59
    • 78751534394 scopus 로고    scopus 로고
    • Trypanocidal drugs: mechanisms, resistance and new targets
    • Wilkinson S.R., Kelly J.M. Trypanocidal drugs: mechanisms, resistance and new targets. Expert Rev. Mol. Med. 2009, 11(e31):1-24.
    • (2009) Expert Rev. Mol. Med. , vol.11 , Issue.E31 , pp. 1-24
    • Wilkinson, S.R.1    Kelly, J.M.2
  • 60
    • 0034678059 scopus 로고    scopus 로고
    • Distinct mitochondrial and cytosolic enzymes mediate trypanothione-dependent peroxide metabolism in Trypanosoma cruzi
    • Wilkinson S.R., Temperton N.J., Mondragon A., Kelly J.M. Distinct mitochondrial and cytosolic enzymes mediate trypanothione-dependent peroxide metabolism in Trypanosoma cruzi. J. Biol. Chem. 2000, 275:8220-8225.
    • (2000) J. Biol. Chem. , vol.275 , pp. 8220-8225
    • Wilkinson, S.R.1    Temperton, N.J.2    Mondragon, A.3    Kelly, J.M.4
  • 61
    • 0037053395 scopus 로고    scopus 로고
    • The Trypanosoma cruzi enzyme TcGPXI is a glycosomal peroxidase and can be linked to trypanothione reduction by glutathione or tryparedoxin
    • Wilkinson S.R., Meyer D.J., Taylor M.C., Bromley E.V., Miles M.A., Kelly J.M. The Trypanosoma cruzi enzyme TcGPXI is a glycosomal peroxidase and can be linked to trypanothione reduction by glutathione or tryparedoxin. J. Biol. Chem. 2002, 277:17062-17071.
    • (2002) J. Biol. Chem. , vol.277 , pp. 17062-17071
    • Wilkinson, S.R.1    Meyer, D.J.2    Taylor, M.C.3    Bromley, E.V.4    Miles, M.A.5    Kelly, J.M.6
  • 62
    • 0037097060 scopus 로고    scopus 로고
    • TcGPXII, a glutathione-dependent Trypanosoma cruzi peroxidase with substrate specificity restricted to fatty acid and phospholipid hydroperoxides, is localised to the endoplasmic reticulum
    • Wilkinson S.R., Taylor M.C., Touitha S., Mauricio I.L., Meyer D.J., Kelly J.M. TcGPXII, a glutathione-dependent Trypanosoma cruzi peroxidase with substrate specificity restricted to fatty acid and phospholipid hydroperoxides, is localised to the endoplasmic reticulum. Biochem. J. 2002, 364:787-794.
    • (2002) Biochem. J. , vol.364 , pp. 787-794
    • Wilkinson, S.R.1    Taylor, M.C.2    Touitha, S.3    Mauricio, I.L.4    Meyer, D.J.5    Kelly, J.M.6
  • 63
    • 0037108740 scopus 로고    scopus 로고
    • Trypanosoma cruzi expresses a plant-like ascorbate-dependent haemoperoxidase localized to the endoplasmic reticulum
    • Wilkinson S.R., Obado S.O., Mauricio I.L., Kelly J.M. Trypanosoma cruzi expresses a plant-like ascorbate-dependent haemoperoxidase localized to the endoplasmic reticulum. Proc. Natl. Acad. Sci. USA 2002, 99:13453-13458.
    • (2002) Proc. Natl. Acad. Sci. USA , vol.99 , pp. 13453-13458
    • Wilkinson, S.R.1    Obado, S.O.2    Mauricio, I.L.3    Kelly, J.M.4
  • 65
    • 0028989439 scopus 로고
    • Inducible gene expression in trypanosomes mediated by a prokaryotic repressor
    • Wirtz E., Clayton C. Inducible gene expression in trypanosomes mediated by a prokaryotic repressor. Science 1995, 268:1179-1183.
    • (1995) Science , vol.268 , pp. 1179-1183
    • Wirtz, E.1    Clayton, C.2
  • 66
    • 0033525524 scopus 로고    scopus 로고
    • A tightly regulated inducible expression system for conditional gene knock-outs and dominant-negative genetics in Trypanosoma brucei
    • Wirtz E., Leal S., Ochatt C., Cross G.A. A tightly regulated inducible expression system for conditional gene knock-outs and dominant-negative genetics in Trypanosoma brucei. Mol. Biochem. Parasitol. 1999, 99:89-101.
    • (1999) Mol. Biochem. Parasitol. , vol.99 , pp. 89-101
    • Wirtz, E.1    Leal, S.2    Ochatt, C.3    Cross, G.A.4
  • 67
    • 66949138915 scopus 로고    scopus 로고
    • Evaluation of high efficiency gene knockout strategies for Trypanosoma cruzi
    • Xu D., Brandán C.P., Basombrío M.A., Tarleton R.L. Evaluation of high efficiency gene knockout strategies for Trypanosoma cruzi. BMC Microbiol. 2009, 9:90.
    • (2009) BMC Microbiol. , vol.9 , pp. 90
    • Xu, D.1    Brandán, C.P.2    Basombrío, M.A.3    Tarleton, R.L.4
  • 68
    • 0025041798 scopus 로고
    • The promoter for a variant surface glycoprotein gene expression site in Trypanosoma brucei
    • Zomerdijk J.C., Ouellette M., ten Asbroek A.L., Kieft R., Bommer A.M., Clayton C.E., et al. The promoter for a variant surface glycoprotein gene expression site in Trypanosoma brucei. EMBO J. 1990, 9:791-801.
    • (1990) EMBO J. , vol.9 , pp. 791-801
    • Zomerdijk, J.C.1    Ouellette, M.2    ten Asbroek, A.L.3    Kieft, R.4    Bommer, A.M.5    Clayton, C.E.6


* 이 정보는 Elsevier사의 SCOPUS DB에서 KISTI가 분석하여 추출한 것입니다.