Telomeric Repeat Amplification Protocol: Measuring the Activity of the Telomerase

Methods in Molecular Biology

Volumn 735, Issue , 2011, Pages 107-111

  Xin, Huawei ^a  

^a BAYLOR COLLEGE OF MEDICINE   (United States)

Author keywords

Cell proliferation; Malignancy; PCR; Senescence; Telomerase

Indexed keywords

TELOMERASE;

ARTICLE; BIOCATALYSIS; ENZYME ASSAY; GENETICS; HUMAN; LONG TERMINAL REPEAT; METABOLISM; METHODOLOGY; SENSITIVITY AND SPECIFICITY; TELOMERE;

BIOCATALYSIS; ENZYME ASSAYS; HUMANS; SENSITIVITY AND SPECIFICITY; TELOMERASE; TELOMERE; TERMINAL REPEAT SEQUENCES;

EID: 79960982836     PISSN: 10643745     EISSN: 19406029     Source Type: Book Series    
DOI: 10.1007/978-1-61779-092-8_10     Document Type: Chapter

References (4)

1. Kim NW, Piatyszek MA, Prowse KR, Harley CB, West MD, Ho PL, Coviello GM, Wright WE, Weinrich SL, Shay JW. (1994) Specific association of human telomerase activity with immortal cells and cancer. Science. 266 (5193):2011– 5.
2. Kim NW, Wu F. (1997) Advances in quantification and characterization of telomerase activity by the telomeric repeat amplification protocol (TRAP). Nucleic Acids Res. 25(13):2595–7.
3. Burger AM. (2002) Standard TRAP assay. Methods Mol Biol. 191:109 –24.
4. Fajkus J. (2006) Detection of telomerase activity by the TRAP assay and its variants and alternatives. Clin Chim Acta. 371(1-2):25 –31.