Laser-Capture Microdissection and Transcriptional Profiling in Archival FFPE Tissue in Prostate Cancer

Methods in Molecular Biology

Volumn 755, Issue , 2011, Pages 291-300

  Joseph, Ajay ^a   Gnanapragasam, Vincent J ^a  

^a HUTCHISON MRC RESEARCH CENTRE   (United Kingdom)

Author keywords

Diagnostic needle biopsies; FFPE; Heterogeneous tissue; LCM; Prognostic marker; Prostate cancer; QPCR; TRUS

Indexed keywords

FIXATIVE; FORMALDEHYDE; PARAFFIN; RNA;

ARTICLE; CHEMISTRY; GENE EXPRESSION PROFILING; GENETICS; HUMAN; INSTRUMENTATION; ISOLATION AND PURIFICATION; LASER; MALE; METHODOLOGY; MICRODISSECTION; PATHOLOGY; PROGNOSIS; PROSTATE TUMOR; REVERSE TRANSCRIPTION POLYMERASE CHAIN REACTION; STAINING; TISSUE FIXATION;

FIXATIVES; FORMALDEHYDE; GENE EXPRESSION PROFILING; HUMANS; LASERS; MALE; MICRODISSECTION; PARAFFIN EMBEDDING; PROGNOSIS; PROSTATIC NEOPLASMS; REVERSE TRANSCRIPTASE POLYMERASE CHAIN REACTION; RNA; STAINING AND LABELING; TISSUE FIXATION;

EID: 79960965892     PISSN: 10643745     EISSN: 19406029     Source Type: Book Series    
DOI: 10.1007/978-1-61779-163-5_24     Document Type: Chapter

References (15)

1. Heidenreich A, Aus G, Bolla M, Joniau S, Matveev VB, Schmid HP, and Zattoni F. (2008) EAU guidelines on prostate cancer, Eur Urol 53, 68–80.
2. Verhagen PC, Tilanus MG, de Weger RA, van Moorselaar RJ, van den Tweel JG, and Boon TA. (2002) Prognostic factors in localised prostate cancer with emphasis on the application of molecular techniques, Eur Urol 41, 363–371.
3. Schlomm T, Erbersdobler A, Mirlacher M, and Sauter G. (2007) Molecular staging of prostate cancer in the year 2007, World J Urol 25, 19–30.
4. Player A, Barrett JC, and Kawasaki ES. (2004) Laser capture microdissection, microarrays and the precise definition of a cancer cell, Expert Rev Mol Diagn 4, 831–840.
5. Rogerson L, Darby S, Jabbar T, Mathers ME, Leung HY, Robson CN, Sahadevan K, O’Toole K, and Gnanapragasam VJ. (2008) Application of transcript profiling in formalin-fixed paraffin-embedded diagnostic prostate cancer needle biopsies, BJU Int 102, 364–370.
6. Rodriguez AS, Espina BH, Espina V, and Liotta LA. (2008) Automated laser capture microdissection for tissue proteomics, Methods Mol Biol 441, 71–90.
7. Ladanyi A, Sipos F, Szoke D, Galamb O, Molnar B, and Tulassay Z. (2006) Laser microdissection in translational and clinical research, Cytometry A 69, 947–960.
8. Livak KJ, and Schmittgen TD. (2001) Analysis of relative gene expression data using real-time quantitative PCR and the 2(−Delta Delta C(T)) Method, Methods 25, 402–408.
9. Polacek DC, Passerini AG, Shi C, Francesco NM, Manduchi E, Grant GR, Powell S, Bischof H, Winkler H, Stoeckert CJ, Jr., and Davies PF. (2003) Fidelity and enhanced sensitivity of differential transcription profiles following linear amplification of nanogram amounts of endothelial mRNA, Physiol Genomics 13, 147–156.
10. Vermeulen J, Derveaux S, Lefever S, De Smet E, De Preter K, Yigit N, De Paepe A, Pattyn F, Speleman F, and Vandesompele J. (2009) RNA pre-amplification enables large-scale RT-qPCR gene-expression studies on limiting sample amounts, BMC Res Notes 2, 235.
11. Seth D, Gorrell MD, McGuinness PH, Leo MA, Lieber CS, McCaughan GW, and Haber PS. (2003) SMART amplification maintains representation of relative gene expression: quantitative validation by real time PCR and application to studies of alcoholic liver disease in primates, J Biochem Biophys Methods 55, 53–66.
12. Watson JD, Wang S, Von Stetina SE, Spencer WC, Levy S, Dexheimer PJ, Kurn N, Heath JD, and Miller DM, 3rd. (2008) Complementary RNA amplification methods enhance microarray identification of transcripts expressed in the C. elegans nervous system, BMC Genomics 9, 84.
13. Tomlins SA, Mehra R, Rhodes DR, Shah RB, Rubin MA, Bruening E, Makarov V, and Chinnaiyan AM. (2006) Whole transcriptome amplification for gene expression profiling and development of molecular archives, Neoplasia 8, 153–162.
14. Dafforn A, Chen P, Deng G, Herrler M, Iglehart D, Koritala S, Lato S, Pillarisetty S, Purohit R, Wang M, Wang S, and Kurn N. (2004) Linear mRNA amplification from as little as 5 ng total RNA for global gene expression analysis, Biotechniques 37, 854–857.
15. Kurn N, Chen P, Heath JD, Kopf-Sill A, Stephens KM, and Wang S. (2005) Novel isothermal, linear nucleic acid amplification systems for highly multiplexed applications, Clin Chem 51, 1973–1981.