Precision across the analytical measuring range of a quantitative real-time PCR assay for cytomegalovirus detection among three clinical laboratories

Journal of Clinical Microbiology

Volumn 49, Issue 8, 2011, Pages 3044-3046

  Grys, Thomas E ^a,b   Duquette, Doreen L ^a   White, Bruce ^c   Irish, Cole ^b   Hata, D Jane ^c   Pritt, Bobbi S ^b  

^a Mayo Medical Laboratories New England   (United States)

^b MAYO CLINIC   (United States)

^c MAYO CLINIC   (United States)

Author keywords

[No Author keywords available]

Indexed keywords

ACCURACY; ARTICLE; CLINICAL LABORATORY; CONTROLLED STUDY; CYTOMEGALOVIRUS; CYTOMEGALOVIRUS INFECTION; DATA ANALYSIS; HUMAN; LABORATORY TEST; MOLECULAR PROBE; NONHUMAN; PRIORITY JOURNAL; QUANTITATIVE ANALYSIS; REAL TIME POLYMERASE CHAIN REACTION; REPRODUCIBILITY; VIRUS DETECTION; VIRUS LOAD;

CLINICAL LABORATORY TECHNIQUES; CYTOMEGALOVIRUS; CYTOMEGALOVIRUS INFECTIONS; HUMANS; OBSERVER VARIATION; REAL-TIME POLYMERASE CHAIN REACTION; VIROLOGY;

CYTOMEGALOVIRUS;

EID: 79960964936     PISSN: 00951137     EISSN: 1098660X     Source Type: Journal    
DOI: 10.1128/JCM.00486-11     Document Type: Article

References (12)

1. Brown, M. B., and A. B. Forsythe. 1974. Robust tests for equality of variances. J. Am. Stat. Assoc. 69:364-367.
2. Caliendo, A. M., et al. 2009. A commutable cytomegalovirus calibrator is required to improve the agreement of viral load values between laboratories. Clin. Chem. 55:1701-1710.
3. CLSI. 2010. Quantitative molecular methods for infectious diseases; approved guideline, 2nd ed. CLSI document MM06-A2. Clinical and Laboratory Standards Institute, Wayne, PA.
4. Fryer, J. F., H. A. R. Anderson, P. D. Minor, and the Collaborative Study Group. 2010. Collaborative study to evaluate the proposed 1st WHO International Standard for human cytomegalovirus (HCMV) for nucleic acid amplification (NAT)-based assays. WHO/BS/10.2138. WHO ECBS report. WHO, Geneva, Switzerland.
5. Hayden, R. T., et al. 2008. Multicenter comparison of different real-time PCR assays for quantitative detection of Epstein-Barr virus. J. Clin. Microbiol. 46:157-163.
6. Kellogg, J. A., P. V. Atria, J. C. Sanders, and M. E. Eyster. 2001. Intra- and interlaboratory variabilities of results obtained with the Quantiplex human immunodeficiency virus type 1 RNA bDNA assay, version 3.0. Clin. Diagn. Lab. Immunol. 8:560-563.
7. National Institutes of Standards and Technology. 5 October 2010, posting date. Certification of a standard reference material for cytomegalovirus. 2011. National Institute of Standards and Technology, Gaithersburg, MD.
8. Panel on Antiretroviral Guidelines for Adults and Adolescents. 2011. Guidelines for the use of antiretroviral agents in HIV-1-infected adults and adolescents, p. 9-10. Department of Health and Human services, Washington, DC. http://www.aidsinfo.nih.gov/ContentFiles/AdultandAdolescentGL.pdf. Accessed 10 January 2011.
9. Pang, X. L., et al. 2009. Interlaboratory comparison of cytomegalovirus viral load assays. Am. J. Transplant. 9:258-268.
10. Preiksaitis, J. K., et al. 2009. Interlaboratory comparison of Epstein-Barr virus viral load assays. Am. J. Transplant. 9:269-279.
11. Razonable, R. R., et al. 2001. Comparative quantitation of cytomegalovirus (CMV) DNA in solid organ transplant recipients with CMV infection by using two high-throughput automated systems. J. Clin. Microbiol. 39:4472-4476.
12. Wolff, D. J., D. L. Heaney, P. D. Neuwald, K. A. Stellrecht, and R. D. Press. 2009. Multi-site PCR-based CMV viral load assessment-assays demonstrate linearity and precision, but lack numeric standardization: a report of the Association for Molecular Pathology. J. Mol. Diagn. 11:87-92.