An efficient protocol for genetic transformation of Platycodon grandiflorum with Agrobacterium rhizogenes

Molecular Biology Reports

Volumn 38, Issue 4, 2011, Pages 2307-2313

  Park, Nam Il ^a   Tuan, Pham Anh ^a   Li, Xiaohua ^a   Kim, Yong Kyoung ^a   Yang, Tae Jin ^b   Park, Sang Un ^a  

^a Chungnam National University   (South Korea)

^b Seoul National University   (South Korea)

Author keywords

Agrobacterium rhizogenes; Hairy root culture; Phenolic compound; Platycodon grandiflorum; Transformation

Indexed keywords

KANAMYCIN; PHENOL; PRIMER DNA; SAPONIN; TRITERPENE;

ARTICLE; BACTERIAL STRAIN; CONTROLLED STUDY; COTYLEDON; CULTURE MEDIUM; GENE LOCUS; GENETIC TRANSFORMATION; HAIRY ROOT CULTURE; HAIRY ROOT DISEASE; HISTOCHEMISTRY; NONHUMAN; PHYTOCHEMISTRY; PLANT GENETICS; PLATYCODON; PLATYCODON GRANDIFLORUM; POLYMERASE CHAIN REACTION; PROMOTER REGION; QUANTITATIVE ANALYSIS; REVERSE TRANSCRIPTION POLYMERASE CHAIN REACTION; RHIZOBIUM RHIZOGENES; RNA ANALYSIS; RNA EXTRACTION; GENETICS; GROWTH, DEVELOPMENT AND AGING; HIGH PERFORMANCE LIQUID CHROMATOGRAPHY; ISOLATION AND PURIFICATION; METABOLISM; METHODOLOGY; PLANT ROOT; RHIZOBIUM; TISSUE CULTURE TECHNIQUE;

AGROBACTERIUM RHIZOGENES; PLATYCODON GRANDIFLORUM; PLATYCODON GRANDIFLORUS;

CHROMATOGRAPHY, HIGH PRESSURE LIQUID; DNA PRIMERS; KANAMYCIN; PLANT ROOTS; PLATYCODON; POLYMERASE CHAIN REACTION; RHIZOBIUM; SAPONINS; TISSUE CULTURE TECHNIQUES; TRANSFORMATION, GENETIC; TRITERPENES;

EID: 79960946529     PISSN: 03014851     EISSN: 15734978     Source Type: Journal    
DOI: 10.1007/s11033-010-0363-0     Document Type: Article

References (21)

1. Gurib-Fakim A (2006) Medicinal plants: traditions of yesterday and drugs of tomorrow. Mol Aspects Med 27:1-93
2. Christey MC, Braun RH (2005) Production of hairy root cultures and transgenic plants by Agrobacterium rhizogenes-mediated transformation. Methods Mol Biol 286:47-60
3. Georgiev MI, Pavlov AI, Bley T (2007) Hairy root type plant in vitro systems as sources of bioactive substances. Appl Microbiol Biotechnol 74:1175-1185
4. Srivastava S, Srivastava AK (2007) Hairy root culture for massproduction of high-value secondary metabolites. Crit Rev Biotechnol 27:29-43
5. Takagi K, Lee EB (1972) Pharmacological studies on Platycodon grandiflorum A. DC. Activities of crude platycodin on respiratory and circulatory systems and its other pharmacological activities. Yakugaku Zasshi 92:969-973
6. Lee EB (1973) Pharmacological studies on Platycodon grandiflorum A. DC. IV. A comparison of experimental pharmacological effects of crude platycodin with clinical indications of Platycodi radix. Yakugaku Zasshi 93:1188-1194
7. Nikaido T, Koike K, Mitsunaga K, Saeki T (1999) Two new triterpenoid saponins from Platycodon grandiflorum. Chem Pharm Bull 47:903-904
8. Ishii H, Tori K, Tozyo T, Yoshimura Y (1984) Saponins from roots of Platycodon grandiflorum. Part 2. Isolation and structure of new triterpene glycosides. J Chem Soc, Perkin Trans 1:661-668
9. Ahn JC, Hwang B, Tada H, Ishimaru K, Sasaki K, Shimomura K (1996) Polyacetylenes in hairy roots of Platycodon grandiflorum. Phytochemistry 42:69-72
10. Murashige T, Skoog F (1962) A revised medium for rapid growth and bioassays with tobacco tissue culture. Physiol Plant 15:473-497
11. Edwards K, Johnstone C, Thompson C (1991) A simple and rapid method for the preparation of plant genomic DNA for PCR analysis. Nucleic Acids Res 19:1349
12. Sundaresan V, Springer P, Volpe T, Haward S, Jones JD, Dean C, Ma H, Martienssen R (1995) Patterns of gene action in plant development revealed by enhancer trap and gene trap transposable elements. Genes Dev 9:1797-1810
13. Vanhala L, Hiltunen R, Oksman-Caldentey K-M (1995) Virulence of different Agrobacterium strains on hairy root formation of Hyoscyamus muticus. Plant Cell Rep 14:236-240
14. Mateus L, Cherkaoui S, Christen P, Oksman-Caldentey K-M (2000) Simultaneous determination of scopolamine, hyoscyamine and littorine in plants and different hairy root clones of Hyoscyamus muticus by micellar electrokinetic chromatography. Phytochemistry 54:517-523
15. Tiwari RK, Trivedi M, Guang ZC, Guo G-Q, Zheng G-C (2007) Genetic transformation of Gentiana macrophylla with Agrobacterium rhizogenes: growth and production of secoiridoid glucoside gentiopicroside in transformed hairy root cultures. Plant Cell Rep 26:199-210
16. McDougall B, King P, Wu B, Hostomsky Z, Reinecke M, Robinson W (1998) Dicaffeoylquinic and dicaffeoyltartaric acids are selective inhibitors of human immunodeficiency virus type 1 integrase. Antimicrob Agents Chemother 42:140-146
17. Sawa T, Nakao M, Akaike T, Ono K, Maeda H (1999) Alkylperoxyl radical-scavenging activity of various flavonoids and other phenolic compounds: implications for the anti-tumor-promoter effect of vegetables. J Agric Food Chem 47:397-402
18. Castelluccio C, Paganga G, Melikian N, Paul Bolwell G, Pridham J, Sampson J, Rice-Evans C (1995) Antioxidant potential of intermediates in phenylpropanoid metabolism in higher plants. FEBS Lett 368:188-192
19. Kono Y, Shibata H, Kodama Y, Sawa Y (1995) The suppression of the N-nitrosating reaction by chlorogenic acid. Biochem J 312:947-953
20. Kasai H, Fukada S, Yamaizumi Z, Sugie S, Mori H (2000) Action of chlorogenic acid in vegetables and fruits as an inhibitor of 8-hydroxydeoxyguanosine formation in vitro and in a rat carcinogenesis model. Food Chem Toxicol 38:467-471
21. Shibata H, Sakamoto Y, Oka M, Kono Y (1999) Natural antioxidant, chlorogenic acid, protects against DNA breakage caused by monochloramine. Biosci Biotechnol Biochem 63:1295-1297