Functional Screening with a Live Cell Imaging-Based Random Cell Migration Assay

Methods in Molecular Biology

Volumn 769, Issue , 2011, Pages 435-448

  van Roosmalen, Wies ^b   Le Dévédec, Sylvia E ^b   Zovko, Sandra ^b   de Bont, Hans ^b   van de Water, Bob ^a  

^a LEIDEN UNIVERSITY   (Netherlands)

^b NONE

Author keywords

Automation; Functional genomics; High throughput screening; Quantitative image analysis; Random cell migration

Indexed keywords

EPIDERMAL GROWTH FACTOR; GREEN FLUORESCENT PROTEIN; HYBRID PROTEIN; PHOSPHATIDYLINOSITOL 3,4,5 TRISPHOSPHATE 3 PHOSPHATASE; PTEN PROTEIN, HUMAN;

ARTICLE; CELL MIGRATION; CELL MOTION; COMPUTER INTERFACE; CULTURE MEDIUM; CULTURE TECHNIQUE; FLUORESCENCE MICROSCOPY; GENE SILENCING; GENETICS; HUMAN; IMAGE PROCESSING; METABOLISM; METHODOLOGY; TUMOR CELL LINE;

CELL CULTURE TECHNIQUES; CELL LINE, TUMOR; CELL MIGRATION ASSAYS; CELL MOVEMENT; CULTURE MEDIA; EPIDERMAL GROWTH FACTOR; GENE KNOCKDOWN TECHNIQUES; GREEN FLUORESCENT PROTEINS; HUMANS; IMAGE PROCESSING, COMPUTER-ASSISTED; MICROSCOPY, FLUORESCENCE; PTEN PHOSPHOHYDROLASE; RECOMBINANT FUSION PROTEINS; USER-COMPUTER INTERFACE;

EID: 79960918842     PISSN: 10643745     EISSN: 19406029     Source Type: Book Series    
DOI: 10.1007/978-1-61779-207-6_29     Document Type: Chapter

References (12)

1. Chambers, A. F., Groom, A. C., and MacDonald, I. C. (2002) Dissemination and growth of cancer cells in metastatic sites. Nat Rev Cancer. 2:563–572.
2. Carragher, N. O. and Frame, M. C. (2004) Focal adhesion and actin dynamics: a place where kinases and proteases meet to promote invasion. Trends Cell Biol. 14: 241–249.
3. Ridley, A. J., Schwartz, M. A., Burridge, K., Firtel, R. A., Ginsberg, M. H., Borisy, G., Parsons, J. T., and Horwitz, A. R. (2003) Cell migration: integrating signals from front to back. Science. 302:1704–1709.
4. Webb, D. J., Parsons, J. T., and Horwitz, A. F. (2002) Adhesion assembly, disassembly and turnover in migrating cells – over and over and over again. Nat. Cell Biol. 4:E97-100.
5. Le Dévédec, S. E., Yan, K., de Bont, H., Ghotra, V., Truong, H., Danen, E. H., Verbeek, F., and van de Water, B. (2010) Systems microscopy approaches to understand cancer cell migration and metastasis. Cell Mol Life Sci. 67:3219–3240.
6. Neumann, B., Held, M., Liebel, U., Erfle, H., Rogers, P., Pepperkok, R., and Ellenberg, J. (2006) High-throughput RNAi screening by time-lapse imaging of live human cells. Nat Methods. 3:385–390.
7. Niederlein, A., Meyenhofer, F., White, D., and Bickle, M. (2009) Image analysis in high-content screening. Comb. Chem High Throughput. Screen. 12:899–907.
8. Pepperkok, R. and Ellenberg, J. (2006) High-throughput fluorescence microscopy for systems biology. Nat Rev Mol Cell Biol. 7:690–696.
9. Zanella, F., Lorens, J. B., and Link, W. (2010) High content screening: seeing is believing. Trends Biotechnol. 28: 237–245.
10. Neri, A., Welch, D., Kawaguchi, T., and Nicolson, G. L. (1982) Development and biologic properties of malignant cell sublines and clones of a spontaneously metastasizing rat mammary adenocarcinoma. J. Natl. Cancer Inst. 68:507–517.
11. Xue, C., Wyckoff, J., Liang, F., Sidani, M., Violini, S., Tsai, K. L., Zhang, Z. Y., Sahai, E., Condeelis, J., and Segall, J. E. (2006) Epidermal growth factor receptor overexpression results in increased tumor cell motility in vivo coordinately with enhanced intravasation and metastasis. Cancer Res. 66:192–197.
12. Ciernik, I. F., Berzofsky, J. A., and Carbone, D. P. (1996) Human lung cancer cells endog-enously expressing mutant p53 process and present the mutant epitope and are lysed by mutant-specific cytotoxic T lymphocytes. Clin Cancer Res. 2:877–882.