Quantitative analysis of dynamic protein-protein interactions in planta by a floated-leaf luciferase complementation imaging (FLuCI) assay using binary Gateway vectors

Plant Journal

Volumn 67, Issue 3, 2011, Pages 542-553

  Gehl, Christian ^a   Kaufholdt, David ^a   Hamisch, Domenica ^a   Bikker, Rolf ^a   Kudla, Jörg ^b   Mendel, Ralf R ^a   Hänsch, Robert ^a  

^a TECHNICAL UNIVERSITY OF BRAUNSCHWEIG   (Germany)

^b UNIVERSITY OF MÜNSTER   (Germany)

Author keywords

floated leaf assay; Gateway binary vectors; GUS calibration; Lifeact; luciferase complementation imaging; Rhizobium tumefaciens transformation

Indexed keywords

COMPLEMENTATION; FLOATED-LEAF ASSAY; GUS CALIBRATION; LIFEACT; TUMEFACIENS;

ASSAYS; BIOCHEMISTRY; CLONING; DYNAMIC ANALYSIS; GATEWAYS (COMPUTER NETWORKS); INVESTMENTS; MOLYBDENUM; MOLYBDENUM COMPOUNDS; PLANTS (BOTANY); VECTORS;

PROTEINS;

ARABIDOPSIS PROTEIN; CNX PROTEIN, ARABIDOPSIS; HYBRID PROTEIN; LUCIFERASE; MOLYBDOPTERIN SYNTHASE; PROTEIN 14 3 3; SULFURTRANSFERASE; VEGETABLE PROTEIN;

ARTICLE; CONFOCAL MICROSCOPY; GENE VECTOR; GENETICS; IMMUNOBLOTTING; LUMINESCENCE; METABOLISM; METHODOLOGY; PLANT LEAF; PROTEIN ANALYSIS; PROTEIN BINDING; PROTEIN MULTIMERIZATION; RHIZOBIUM; TOBACCO;

14-3-3 PROTEINS; ARABIDOPSIS PROTEINS; GENETIC VECTORS; IMMUNOBLOTTING; LUCIFERASES; LUMINESCENT MEASUREMENTS; MICROSCOPY, CONFOCAL; PLANT LEAVES; PLANT PROTEINS; PROTEIN BINDING; PROTEIN INTERACTION MAPPING; PROTEIN MULTIMERIZATION; RECOMBINANT FUSION PROTEINS; RHIZOBIUM; SULFURTRANSFERASES; TOBACCO;

NICOTIANA BENTHAMIANA; RHIZOBIUM;

EID: 79960918742     PISSN: 09607412     EISSN: 1365313X     Source Type: Journal    
DOI: 10.1111/j.1365-313X.2011.04607.x     Document Type: Article

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