The dominant glutamic acid metabolic flux to produce γ-amino butyric acid over proline in nicotiana tabacum leaves under water stress relates to its significant role in antioxidant activity

Journal of Integrative Plant Biology

Volumn 53, Issue 8, 2011, Pages 608-618

  Liu, Cuili ^a   Zhao, Li ^a   Yu, Guanghui ^a  

^a SOUTH CENTRAL UNIVERSITY FOR NATIONALITIES   (China)

Author keywords

amino butyric acid; Glutamic acid; Proline; Reactive oxygen species; Water stress

Indexed keywords

4 AMINOBUTYRIC ACID; ANTIOXIDANT; GLUTAMATE DECARBOXYLASE; GLUTAMATE GAMMA SEMIALDEHYDE DEHYDROGENASE; GLUTAMIC ACID; HYDROGEN PEROXIDE; PROLINE; SCAVENGER; SINGLET OXYGEN; SUPEROXIDE; WATER;

AMINO ACID; ANTIOXIDANT; ENVIRONMENTAL STRESS; ENZYME ACTIVITY; LEAF; METABOLISM; OSMOSIS; POLYMERASE CHAIN REACTION; QUANTITATIVE ANALYSIS; REACTION KINETICS; SCAVENGING (CHEMISTRY); TOBACCO; WATER STRESS;

ARTICLE; DEHYDRATION; ENZYMOLOGY; GENE EXPRESSION REGULATION; GENETICS; METABOLISM; PLANT LEAF; TOBACCO;

ANTIOXIDANTS; DEHYDRATION; FREE RADICAL SCAVENGERS; GAMMA-AMINOBUTYRIC ACID; GENE EXPRESSION REGULATION, ENZYMOLOGIC; GENE EXPRESSION REGULATION, PLANT; GLUTAMATE DECARBOXYLASE; GLUTAMATE-5-SEMIALDEHYDE DEHYDROGENASE; GLUTAMIC ACID; HYDROGEN PEROXIDE; PLANT LEAVES; PROLINE; SINGLET OXYGEN; SUPEROXIDES; TOBACCO; WATER;

NICOTIANA TABACUM;

EID: 79960858882     PISSN: 16729072     EISSN: 17447909     Source Type: Journal    
DOI: 10.1111/j.1744-7909.2011.01049.x     Document Type: Article

References (41)

1. Alia MP, Matysik J (2001) Effect of proline on the production of singlet oxygen. Amino Acids 21, 195-200.
2. Baum G, Lev-Yadun S, Fridmann Y, Arazi T, Katsnelson H, Zik M, Fromm H (1996) Calmodulin binding to glutamate decarboxylase is required for regulation of glutamate and GABA metabolism and normal development in plants. EMBO J. 15, 2988-2996.
3. Bouché N, Fromm H (2004) GABA in plants: Just a metabolite? Trends Plant Sci. 9, 110-115.
4. Bouché N, Fait A, Zik M, Fromm H (2004) The root-specific glutamate decarboxylase (GAD1) is essential for sustaining GABA levels in Arabidopsis. Plant Mol. Biol. 3, 315-325.
5. Boyer JS (1982) Plant productivity and environment. Science 218, 443-448.
6. Chen HH, Ran GX, Xue LD, Song QJ (2008) Methylene blue immobilized and L-trptophan oxidation in heterogeneous photosensitization system. World Sci-tech. R&D. 30, 681-683.
7. Chen Y, Baum G, Fromm H (1994) The 58-kilodalton calmodulin-binding glutamate decarboxylase is a ubiquitous protein in petunia organs and its expression is developmentally regulated. Plant Physiol. 106, 1381-1387.
8. 2+ sensors in plant signalling. Biochem. J. 425, 27-40.
9. Delauney AJ, Hu CAA, Kishor PBK, Verma DPS (1993) Cloning of ornithine delta-aminotransferase cDNA from Vigna aconitifolia by trans-complementation in Escherichia coli and regulation of proline biosynthesis. J. Biol. Chem. 268, 18673-18678.
10. Forde BG, Lea PJ (2007) Glutamate in plants: Metabolism, regulation, and signalling. J. Exp. Bot. 58, 2339-2358.
11. Garcia-Rios M, Fujita T, LaRosa PC, Locy R, Clithero JM, Bressan RA, Csonka LN (1997) Cloning of a polycistronic cDNA from tomato encoding γ-glutamyl kinase and γ-glutamyl phosphate reductase. Proc. Natl. Sci. USA. 94, 8249-8254.
12. Hare PD, Cress WA (1997) Metabolic implications of stress-induced proline accumulation in plants. Plant Growth Regul. 21, 79-102.
13. Hong ZL, Lakkineni K, Zhang ZH, Verma DPS (2000) Removal of feedback inhibition of Δ1-Pyrroline-5.-Carboxylate synthetase results in increased proline accumulation and protection of plants from osmotic stress. Plant Physiol. 122, 1129-1136.
14. Johnson BS, Singh NK, Cherry JH, Locy RD (1997) Purification and characterization of glutamate decarboxylase from cowpea. Phytochem. 46, 39-44.
15. Kaul S, Sharma SS, Mehta IK (2008) Free radical scavenging potential of L-proline: evidence from in vitro assays. Amino Acids 34, 315-320.
16. B receptors uncovers similarity to metabotropic glutamate receptors. Nature 386, 239-246.
17. 1-pyrroline-5-carboxylate synthetase increases proline overproduction and confers osmotolerance in transgenic plants. Plant Physiol. 108, 1387-1394.
18. Knight H, Trewaves AJ, Knight MR (1997) Calcium signaling in Arabidopsis thaliana responding to drought and salinity. Plant J. 12, 1067-1078.
19. Knox JP Dodge AD (1985) The photodynamic action of eosin, a singlet-oxygen generator. Planta 164, 22-29.
20. Levitt J (1986) Recovery of turgor by wilted, excised cabbage leaves in the absence of water uptake. Plant Physiol. 82, 147-153.
21. Lipkowitz KB, Gilardi RD, Aprison MH (1989) Electronic and structural features of γ-aminobutyric acid (GABA) and four of its direct agonists. J. Mol. Struct. 195, 65-77.
22. -ΔΔCT method. Methods 25, 402-408.
23. Martinez-Garcia JF, Monte E, Quail PH (1999) A simple, rapid and quantitative method for preparing Arabidopsis protein extracts for immunoblot analysis. Plant J. 20, 251-257.
24. Mattoo AK, Minocha SC, Minocha R, Handa AK (2010) Polyamines and cellular metabolism in plants: Transgenic approaches reveal different responses to diamine putrescine versus higher polyamines spermidine and spermine. Amino Acids 38, 405-413.
25. Matysik J, Alia, Bhalu B, Mohanty P (2002) Molecular mechanisms of quenching of reactive oxygen species by proline under stress in plants. Curr. Sci. 82, 525-532.
26. Mccaig TN, Romagosa I (1991) Water status measurements of excised wheat leaves: Position and age effects. Crop Sci. 31, 1583-1588.
27. Nanda AK, Andrio E, Marino D, Pauly N, Dunand C (2010) Reactive oxygen species during plant-microorganism early interactions. J. Integr. Plant Biol. 52, 195-204.
28. Palanivelu R, Brass L, Edlund AF, Preuss D (2003) Pollen tube growth and guidance is regulated by POP2, an Arabidopsis gene that controls GABA levels. Cell 114, 47-59.
29. Quan LJ, Zhang B, Shi WW, Li HY (2008) Hydrogen peroxide in plants: A versatile molecule of the reactive oxygen species network. J. Integr. Plant Biol. 50, 2-18.
30. Shelp B, Bown A, McLean M (1999) Metabolism and functions of gamma-aminobutyric acid. Trends Plant Sci. 4, 446-452.
31. Silva-Ortega CO, Ochoa-Alfaro AE, Reyes-Agüero JA, Aguado-Santacruz GA, Jiménez-Bremont JF (2008) Salt stress increases the expression of p5cs gene and induces proline accumulation in cactus pear. Plant Physiol. Biochem. 46, 82-92.
32. Smirnoff N, Cumbes QJ (1989) Hydroxyl radical scavenging activity of compatible solutes. Phytochemistry 28, 1057-1060.
33. Snedden WA, Koutsia N, Baum G, Fromm H (1996) Activation of a recombinant petunia glutamate decarboxylase by calcium/calmodulin or by a monoclonal antibody, which recognizes the calmodulin-binding domain. J. Biol. Chem. 271, 4148-4153.
34. Székely G, Abrahám E, Cséplo A, Rigó G, Zsigmond L, Csiszár J, Ayaydin F, Strizhov N, Jásik J, Schmelzer E, Koncz C, Szabados L (2008) Duplicated P5CS genes of Arabidopsis play distinct roles in stress regulation and developmental control of proline biosynthesis. Plant J. 53, 11-28.
35. Wang SJ, Liu DQ, Jiang F, Wang SW (2004) Photometry with oxidation of DPCI for the detection of singlet oxygen. J. Guangdong Univ. Technol. (Sci. Edi.). 21(3), 11-14.
36. 2 alleviates aluminum-induced oxidative stress in wheat seedlings. J. Integr. Plant Biol. 53, 44-53.
37. Yevtushenko DP, McLean MD, Peiris S, Van Cauwenberghe OR, Shelp BJ (2003) Calcium/calmodulin activation of two divergent glutamate decarboxylases from tobacco. J. Exp. Bot. 54, 2001-2002.
38. Yu GH, Sun MX (2007) Deciphering the possible mechanism of GABA in tobacco pollen tube growth and guidance. Plant Signal. Behav. 2, 393-395.
39. Yu GH, Liang JG, He ZK, Sun MX (2006). Quantum dot-mediated detection of γ-aminobutyric acid binding sites on the surface of living pollen protoplasts in tobacco. Chem. Biol. 13, 723-731.
40. Yuan SJ, Stewart CN (2005) Real-time PCR statistics. PCR Encyclopedia. 1, 101127-101149.
41. 1-pyrroline-5-carboxylate synthetase, a bifunctional enzyme catalyzing the first two steps of proline biosynthesis in plants. J. Biol. Chem. 270, 20491-20496.