Cloning of chitinase gene from Bacillus thuringiensis

Indian Journal of Biotechnology

Volumn 10, Issue 3, 2011, Pages 264-269

  Usharani, T R ^a   Gowda, T K S ^a  

^a UNIVERSITY OF AGRICULTURAL SCIENCES   (India)

Author keywords

Bacillus thuringiensis; Colloidal chitin; Exochitinase; Fungitoxicity; Native PAGE

Indexed keywords

AMINO ACID; CHITIN; CHITINASE; GLYCOL; POLYPEPTIDE;

ARTICLE; BACILLUS THURINGIENSIS; BACTERIUM ISOLATION; CLONING; CONTROLLED STUDY; DEGRADATION; DNA SEQUENCE; ENZYME ACTIVITY; FLUORESCENCE MICROSCOPY; FUSARIUM OXYSPORUM; NONHUMAN; NUCLEOTIDE SEQUENCE; POLYMERASE CHAIN REACTION;

BACILLUS CEREUS; BACILLUS THURINGIENSIS; FUSARIUM OXYSPORUM F. CUBENSE;

EID: 79960832883     PISSN: 09725849     EISSN: 09750967     Source Type: Journal    
DOI: None     Document Type: Article

References (19)

1. de Maagd R A, Bosch D & Stiekema W, Bacillus thuringiensis toxin mediated insect resistance in plants, Trends Plant Sci, 4 (1999) 9-13
2. Kobayashi D Y, Reedy R M, Bick J & Oudemans P V, Characterization of a chitinase gene from Stenotrophomonas maltophilia strain 34S1 and its involvement in biological control, Appl Environ Microbiol, 68 (2002) 1047-1054
3. Gooday G W, The ecology of chitin decomposition, Adv Microb Ecol, 11 (1990) 387-430
4. Folders J, Algra J, Roelofs M S, Van loon L C, Tommassen J et al, Characterization of Pseudomonas aeruginosa chitinase, a gradually secreted protein, J Bacteriol, 183 (2001) 7044-7052
5. Uchiyama T, Katouno F, Nikaidou N, Nonaka T, Sugiyama J et al, Roles of the exposed aromatic residues in crystalline chitin hydrolysis by chitinase A from Serratia marcescens 2170, J Biol Chem, 276 (2001) 41343-41349
6. Okazaki K, Yamashita Y, Noda M, Sueyoshi N, Kameshita I et al, Molecular cloning and expression of the gene encoding family 19 chitinase from Streptomyces sp. J-13-3, Biosci Biotechnol Biochem, 68 (2004) 341-51.
7. Barboza-Corona E J, Nieto-Mazzocco E, Velázquez-Robledo R, Salcedo-Hernandez R, Bautista M et al, Cloning, sequencing, and expression of the chitinase gene chiA74 from Bacillus thuringiensis, Appl Environ Microbiol, 69 (2003) 1023-1029
8. Bendt A, Huller H, Kammel U, Helmke E & Schweder T, Cloning, expression, and characterization of a chitinase gene from the Antarctic psychrotolerant bacterium Vibrio sp. strain Fi:7, Extremophiles, 5 (2001) 119-26.
9. Watanabe T, Ito Y, Yamada T, Hashimoto M, Sekine S et al, The role of the C-terminal domain and type III domains of chitinase A1 from Bacillus circulans WL-12 in chitin degradation, J Bacteriol, 176 (1994) 4465-4472
10. Lertcanawanichakul M, Wiwat C, Bhimiratana A & Dean D H, Expression of chitinase-encoding genes in Bacillus thuringiensis and toxicity of engineered B. thuringiensis subsp. aizawai toward Lymantria dispar Larvae, Curr Microbiol, 48 (2004) 175-181.
11. Roberts W K & Selitrennikoff C P, Plant and bacterial chitinase differ in antifungal activity, J Gen Microbiol, 34 (1988) 169-176
12. Shimahara K & Takiguchi Y, Preparation of crustacean chitin, Methods Enzymol, 161 (1988) 417-423
13. Boller T & Mauch F, Colorimetric assay for chitinase, Method Enzymol, 161 (1988) 430-435
14. Loffler B M & Kunze H, Refinement of the coomassie brilliant blue-G assay for quantitative protein determination, Anal Biochem, 177 (1989) 100-102
15. Davis B J, Disc electrophoresis II. Method and application to human serum proteins, Ann N Y Acad Sci, 121 (1964) 404-427.
16. Trudel J & Asselin A, Detection of chitinase activity after polyacrylamide gel electrophoresis, Anal Biochem, 178 (1989) 362-366
17. Ausubel F M, Brent R, Kingston R E, Moore D D, Seidman J G et al, Short protocols in molecular biology (John Wiley & Sons, New York) 1999, 2-13
18. Wen C, Tseng C, Cheng C & Li Y, Purification, characterization and cloning of a chitinase from Bacillus sp. NCTU2, Biotechnol Appl Biochem, 35 (2002) 213-219.
19. Zhong W, Fang J, Ping-Zhong Cai P, Yan W, Wu J et al, Cloning of the Bacillus thuringiensis serovar sotto chitinase (Schi) gene and characterization of its protein, Genet Mol Biol, 28 (2005) 821-826