Rapid fluorescence-based screening for Wolbachia endosymbionts in Drosophila germ line and somatic tissues

Applied and Environmental Microbiology

Volumn 77, Issue 14, 2011, Pages 4788-4794

  Casper Lindley, Catharina ^a   Kimura, Scott ^a   Saxton, Daniel S ^a,b   Essaw, Yonathan ^a   Simpson, Isaac ^a   Tan, Vinson ^a   Sullivan, William ^a  

^a UNIVERSITY OF CALIFORNIA   (United States)

^b LEWIS AND CLARK COLLEGE   (United States)

Author keywords

[No Author keywords available]

Indexed keywords

CALIFORNIA; CELLULAR DISTRIBUTIONS; ENDOSYMBIONT; FLUORESCENT DYES; GERM LINE; INSECT POPULATION; SCREENING APPROACHES; SOMATIC TISSUES;

BACTERIOLOGY; FLUORESCENCE; HISTOLOGY; TISSUE;

BACTERIA;

BACTERIAL DNA; NUCLEIC ACID;

ARTHROPOD; BACTERIAL DISEASE; BACTERIUM; EGG; ENDOSYMBIONT; FLY; IDENTIFICATION KEY; INFECTIOUS DISEASE; NEMATODE; POLYMERASE CHAIN REACTION;

ANIMAL; ARTICLE; DROSOPHILA MELANOGASTER; FLUORESCENCE MICROSCOPY; GENETICS; GERM CELL; ISOLATION AND PURIFICATION; METABOLISM; METHODOLOGY; MICROBIOLOGY; POLYMERASE CHAIN REACTION; SPIROPLASMA; SYMBIOSIS; TIME; WOLBACHIA;

ANIMALS; DNA, BACTERIAL; DROSOPHILA MELANOGASTER; GERM CELLS; MICROSCOPY, FLUORESCENCE; NUCLEIC ACIDS; POLYMERASE CHAIN REACTION; SPIROPLASMA; SYMBIOSIS; TIME FACTORS; WOLBACHIA;

CALIFORNIA; UNITED STATES;

ARTHROPODA; BACTERIA (MICROORGANISMS); CANDIDATUS CARDINIUM; HEXAPODA; NEMATODA; SPIROPLASMA; WOLBACHIA;

EID: 79960711727     PISSN: 00992240     EISSN: 10985336     Source Type: Journal    
DOI: 10.1128/AEM.00215-11     Document Type: Article

References (28)

1. Albertson, R., C. Casper-Lindley, J. Cao, U. Tram, and W. Sullivan. 2009. Symmetric and asymmetric mitotic segregation patterns influence Wolbachia distribution in host somatic tissue. J. Cell Sci. 122:4570-4583.
2. Arthofer, W., et al. 2009. Hidden Wolbachia diversity in field populations of the European cherry fruit fly, Rhagoletis cerasi (Diptera, Tephritidae). Mol. Ecol. 18:3816-3830.
3. Clark, M. E., C. L. Anderson, J. Cande, and T. L. Karr. 2005. Widespread prevalence of Wolbachia in laboratory stocks and the implications for Drosophila research. Genetics 170:1667-1675.
4. Cole, R. M., J. G. Tully, T. J. Popkin, and J. M. Bove. 1973. Morphology, ultrastructure, and bacteriophage infection of the helical mycoplasma-like organism (Spiroplasma citri gen. nov., sp. nov.) cultured from "stubborn" disease of citrus. J. Bacteriol. 115:367-384.
5. Dobson, S. L., et al. 1999. Wolbachia infections are distributed throughout insect somatic and germ line tissues. Insect Biochem. Mol. Biol. 29:153-160.
6. Duron, O., G. D. Hurst, E. A. Hornett, J. A. Josling, and J. Engelstadter. 2008. High incidence of the maternally inherited bacterium Cardinium in spiders. Mol. Ecol. 17:1427-1437.
7. Ferree, P. M., et al. 2005. Wolbachia utilizes host microtubules and dynein for anterior localization in the Drosophila oocyte. PLoS Pathog. 1:e14.
8. Frydman, H. M., J. M. Li, D. N. Robson, and E. Wieschaus. 2006. Somatic stem cell niche tropism in Wolbachia. Nature 441:509-512.
9. Hilgenboecker, K., P. Hammerstein, P. Schlattmann, A. Telschow, and J. H. Werren. 2008. How many species are infected with Wolbachia? A statistical analysis of current data. FEMS Microbiol. Lett. 281:215-220.
10. Hoffmann, A. A., M. Turelli, and L. G. Harshman. 1990. Factors affecting the distribution of cytoplasmic incompatibility in Drosophila simulans. Genetics 126:933-948.
11. Hoffmann, A. A., M. Hercus, and H. Dagher. 1998. Population dynamics of the Wolbachia infection causing cytoplasmic incompatibility in Drosophila melanogaster. Genetics 148:221-231.
12. Hoshizaki, S., and T. Shimada. 1995. PCR-based detection of Wolbachia, cytoplasmic incompatibility microorganisms, infected in natural populations of Laodelphax striatellus (Homoptera: Delphacidae) in central Japan: has the distribution of Wolbachia spread recently? Insect Mol. Biol. 4:237-243.
13. Hosokawa, T., R. Koga, Y. Kikuchi, X. Y. Meng, and T. Fukatsu. 2010. Wolbachia as a bacteriocyte-associated nutritional mutualist. Proc. Natl. Acad. Sci. U. S. A. 107:769-774.
14. Jeyaprakash, A., and M. A. Hoy. 2000. Long PCR improves Wolbachia DNA amplification: wsp sequences found in 76% of sixty-three arthropod species. Insect Mol. Biol. 9:393-405.
15. Kremer, N., et al. 2009. Wolbachia interferes with ferritin expression and iron metabolism in insects. PLoS Pathog. 5:e1000630.
16. Landmann, F., J. M. Foster, B. Slatko, and W. Sullivan. 2010. Asymmetric Wolbachia segregation during early Brugia malayi embryogenesis determines its distribution in adult host tissues. PLoS Negl. Trop. Dis. 4:e758.
17. Mateos, M., et al. 2006. Heritable endosymbionts of Drosophila. Genetics 174:363-376.
18. McGraw, E. A., D. J. Merritt, J. N. Droller, and S. L. O'Neill. 2002. Wolbachia density and virulence attenuation after transfer into a novel host. Proc. Natl. Acad. Sci. U. S. A. 99:2918-2923.
19. Miller, W. J., L. Ehrman, and D. Schneider. 2010. Infectious speciation revisited: impact of symbiont-depletion on female fitness and mating behavior of Drosophila paulistorum. PLoS Pathog. 6:e1001214.
20. O'Neill, S. L., R. Giordano, A. M. Colbert, T. L. Karr, and H. M. Robertson. 1992. 16S rRNA phylogenetic analysis of the bacterial endosymbionts associated with cytoplasmic incompatibility in insects. Proc. Natl. Acad. Sci. U. S. A. 89:2699-2702.
21. Sambrook, J., E. F. Fritsch, and T. Manniatis. 1989. Molecular cloning-a laboratory manual, 2nd ed. Cold Spring Harbor Laboratory, Cold Spring Harbor, NY.
22. Serbus, L. R., C. Casper-Lindley, F. Landmann, and W. Sullivan. 2008. The genetics and cell biology of Wolbachia-host interaction. Annu. Rev. Genet. 42:683-707.
23. Turelli, M., and A. A. Hoffmann. 1995. Cytoplasmic incompatibility in Drosophila simulans: dynamics and parameter estimates from natural populations. Genetics 140:1319-1338.
24. Watts, T., T. S. Haselkorn, N. A. Moran, and T. A. Markow. 2009. Variable incidence of Spiroplasma infections in natural populations of Drosophila species. PLoS One 4:e5703.
25. Weeks, A. R., M. Turelli, W. R. Harcombe, K. T. Reynolds, and A. A. Hoffmann. 2007. From parasite to mutualist: rapid evolution of Wolbachia in natural populations of Drosophila. PLoS Biol. 5:e114.
26. Werren, J. H., D. M. Windsor, and L. R. Guo. 1995. Distribution of Wolbachia among neotropical arthropods. Proc. Biol. Sci. 262:197-204.
27. Zchori-Fein, E., and S. J. Perlman. 2004. Distribution of the bacterial symbiont Cardinium in arthropods. Mol. Ecol. 13:2009-2016.
28. Zchori-Fein, E., S. J. Perlman, S. E. Kelly, N. Katzir, and M. S. Hunter. 2004. Characterization of a 'Bacteroidetes' symbiont in Encarsia wasps (Hymenoptera: Aphelinidae): proposal of 'Candidatus Cardinium hertigii.' Int. J. Syst. Evol. Microbiol. 54:961-968.