Electrophysiological Characterization of Claudin Ion Permeability Using Stably Transfected Epithelial Cell Lines

Methods in Molecular Biology

Volumn 762, Issue , 2011, Pages 27-41

  Yu, Alan S L ^a  

^a UNIVERSITY OF KANSAS MEDICAL CENTER   (United States)

Author keywords

Claudin; Conductance; Diffusion potential; Ion permeability; Junction potential; Paracellular; Tight junction

Indexed keywords

CLAUDIN; ION CHANNEL;

ANIMAL; ARTICLE; CELL LINE; CULTURE TECHNIQUE; DIFFUSION CHAMBER; DOG; ELECTROPHYSIOLOGY; EPITHELIUM CELL; GENETIC TRANSFECTION; METABOLISM; METHODOLOGY; PERMEABILITY; TIGHT JUNCTION;

ANIMALS; CELL CULTURE TECHNIQUES; CELL LINE; CLAUDINS; DIFFUSION CHAMBERS, CULTURE; DOGS; ELECTROPHYSIOLOGY; EPITHELIAL CELLS; ION CHANNELS; PERMEABILITY; TIGHT JUNCTIONS; TRANSFECTION;

EID: 79960709574     PISSN: 10643745     EISSN: 19406029     Source Type: Book Series    
DOI: 10.1007/978-1-61779-185-7_3     Document Type: Chapter

References (12)

1. Tsukita S, Furuse M (2002) Claudin-based barrier in simple and stratified cellular sheets. Curr Opin Cell Biol 14:531–536
2. Van Itallie CM, Anderson JM (2006) Claudins and epithelial paracellular transport. Annu Rev Physiol 68:403–429
3. Angelow S, Ahlstrom R, Yu AS (2008) Biology of claudins. Am J Physiol Renal Physiol 295: F867–876
4. Saier MH, Jr. (1981) Growth and differentiated properties of a kidney epithelial cell line (MDCK). Am J Physiol 240:C106–109
5. Cereijido M, Gonzalez-Mariscal L, Borboa L (1983) Occluding junctions and paracellular pathways studied in monolayers of MDCK cells. J Exp Biol 106:205–215
6. Van Itallie C, Fanning AS, Anderson JM (2003) Reversal of charge selectivity in cation or anion selective epithelial lines by expression of different claudins. Am J Physiol Cell Physiol 286:F1078–1084
7. Angelow S, Schneeberger EE, Yu AS (2007) Claudin-8 expression in renal epithelial cells augments the paracellular barrier by replacing endogenous claudin-2. J Membr Biol 215: 147–159
8. Gossen M, Bujard H (1992) Tight control of gene expression in mammalian cells by tetracy- cline-responsive promoters. Proc Natl Acad Sci U S A 89:5547–5551
9. Barry PH, Diamond JM (1970) Junction potentials, electrode standard potentials, and other problems in interpreting electrical properties of membranes. J Membr Biol 3:93–122
10. Yu AS, Cheng MH, Angelow S, et al. (2009) Molecular basis for cation selectivity in claudin-2-based paracellular pores: identification of an electrostatic interaction site. J Gen Physiol 133:111–127
11. Hille B (2001) Ionic channels of excitable membranes. Sunderland: Sinauer.
12. Kimizuka H, Koketsu K (1964) Ion transport through cell membrane. J Theoret Biol 6:290–305