Growth hormone in sports: Detecting the doped or duped

Hormone Research in Paediatrics

Volumn 76, Issue SUPPL. 1, 2011, Pages 84-90

  Ho, Ken K Y ^a,b,c   Nelson, Anne E ^a  

^a GARVAN INSTITUTE OF MEDICAL RESEARCH   (Australia)

^b ST VINCENT S HOSPITAL   (Australia)

^c PRINCESS ALEXANDRA HOSPITAL   (Australia)

Author keywords

Doping; Growth hormone; IGF I; Procollagen I; Procollagen III

Indexed keywords

CARBOXY TERMINAL TELOPEPTIDE; COLLAGEN; GROWTH HORMONE; GROWTH HORMONE BINDING PROTEIN; INSULIN; OSTEOCALCIN; PLACEBO; SOMATOMEDIN BINDING PROTEIN 1; SOMATOMEDIN BINDING PROTEIN 2; SOMATOMEDIN BINDING PROTEIN 3; SOMATOMEDIN C; STEROID;

ATHLETE; ATHLETIC PERFORMANCE; COLLAGEN SYNTHESIS; COMPARATIVE STUDY; CONFERENCE PAPER; CONNECTIVE TISSUE; DEAMINATION; DOPING; DRUG ACETYLATION; EXERCISE; HEALTH HAZARD; HUMAN; HYPOPHYSIS FUNCTION; PRIORITY JOURNAL; PROTEIN DEGRADATION; PROTEIN PHOSPHORYLATION; RANDOMIZED CONTROLLED TRIAL (TOPIC); SPORT;

AGE FACTORS; ANTIBODIES, MONOCLONAL; COLLAGEN TYPE I; DOPING IN SPORTS; FEMALE; HUMAN GROWTH HORMONE; HUMANS; INSULIN-LIKE GROWTH FACTOR BINDING PROTEIN 3; INSULIN-LIKE GROWTH FACTOR I; MALE; PEPTIDE FRAGMENTS; PEPTIDES; PROTEIN ISOFORMS; PROTEOMICS; RECOMBINANT PROTEINS; SUBSTANCE ABUSE DETECTION;

EID: 79960701784     PISSN: 16632818     EISSN: 16632826     Source Type: Journal    
DOI: 10.1159/000329185     Document Type: Conference Paper

References (54)

1. Nelson AE, Ho KK: Abuse of growth hormone by athletes. Nat Clin Pract Endocrinol Metab 2007; 3: 198-199.
2. Bamberger M, Yaeger D: Over the edge: special report. Sports Illustrated 1997; 86: 64.
3. Rickert VI, Pawlak-Morello C, Sheppard V, Jay MS: Human growth hormone: a new substance of abuse among adolescents? Clin Pediatr (Phila) 1992; 31: 723-726. (Pubitemid 23006319)
4. Johnston LD, O'Malley PM, Bachman JG, Schulenberg JE: Monitoring the Future National Survey Results on Drug Use, 1975-2006: Volume I, Secondary School Students. Bethesda, National Institute on Drug Abuse, 2007, NIH Publication No. 07-6205.
5. NCAA: NCAA Study of Substance Use of College Student-Athletes. Indianapolis, NCAA, 2006.
6. Saugy M, Robinson N, Saudan C, Baume N, Avois L, Mangin P: Human growth hormone doping in sport. Br J Sports Med 2006; 40(suppl 1):i35-i39. (Pubitemid 44032629)
7. Parkinson AB, Evans NA: Anabolic androgenic steroids: a survey of 500 users. Med Sci Sports Exerc 2006; 38: 644-651.
8. Woodhouse LJ, Mukherjee A, Shalet SM, Ezzat S: The influence of growth hormone status on physical impairments, functional limitations, and health-related quality of life in adults. Endocr Rev 2006; 27: 287-317.
9. Healy ML, Gibney J, Russell-Jones DL, Pentecost C, Croos P, Sönksen PH, Umpleby AM: High dose growth hormone exerts an anabolic effect at rest and during exercise in endurance-trained athletes. J Clin Endocrinol Metab 2003; 88: 5221-5226. (Pubitemid 37452719)
10. Liu H, Bravata DM, Olkin I, Friedlander A, Liu V, Roberts B, Bendavid E, Saynina O, Salpeter SR, Garber AM, Hoffman AR: Systematic review: the effects of growth hormone on athletic performance. Ann Intern Med 2008; 148: 747-758. (Pubitemid 351717421)
11. Weissberger AJ, Ho KY, Stuart MC: Quantification of urinary growth hormone (GH) excretion by centrifugal ultrafiltration and radioimmunoassay: appraisal of the relationship between 24 h urinary GH and mean h serum GH levels in normal and abnormal states of GH secretion. Clin Endocrinol (Oxf) 1989; 30: 687-698. (Pubitemid 19152826)
12. Flanagan DE, Taylor MC, Parfitt V, Mardell R, Wood PJ, Leatherdale BA: Urinary growth hormone following exercise to assess growth hormone production in adults. Clin Endocrinol (Oxf) 1997; 46: 425-429. (Pubitemid 27242677)
13. Saugy M, Cardis C, Schweizer C, Veuthey JL, Rivier L: Detection of human growth hormone doping in urine: out of competition tests are necessary. J Chromatogr B Biomed Appl 1996; 687: 201-211. (Pubitemid 27008319)
14. Kazlauskas R, Howe C, Trout G: Strategies for rhEPO detection in sport. Clin J Sport Med 2002; 12: 229-235. (Pubitemid 34747723)
15. Holl RW, Schwarz U, Schauwecker P, Benz R, Veldhuis JD, Heinze E: Diurnal variation in the elimination rate of human growth hormone (GH): the half-life of serum GH is prolonged in the evening, and affected by the source of the hormone, as well as by body size and serum estradiol. J Clin Endocrinol Metab 1993; 77: 216-220. (Pubitemid 23206806)
16. Leung KC, Howe C, Gui LY, Trout G, Veldhuis JD, Ho KK: Physiological and pharmacological regulation of 20-kDa growth hormone. Am J Physiol Endocrinol Metab 2002; 283:E836-E843.
17. Giustina A, Veldhuis JD: Pathophysiology of the neuroregulation of growth hormone secretion in experimental animals and the human. Endocr Rev 1998; 19: 717-797. (Pubitemid 128506521)
18. Gibney J, Healy ML, Sönksen PH: The growth hormone/insulin-like growth factor-I axis in exercise and sport. Endocr Rev 2007; 28: 603-624. (Pubitemid 47610444)
19. Baumann G: Growth hormone heterogeneity in human pituitary and plasma. Horm Res 1999; 51(suppl 1):2-6. (Pubitemid 29301276)
20. Zhan X, Giorgianni F, Desiderio DM: Proteomics analysis of growth hormone isoforms in the human pituitary. Proteomics 2005; 5: 1228-1241.
21. Baxter RC, Brown AS, Turtle JR: Radioimmunoassay for somatomedin C: comparison with radioreceptor assay in patients with growth-hormone disorders, hypothyroidism, and renal failure. Clin Chem 1982; 28: 488-495. (Pubitemid 12145615)
22. Lewis UJ, Sinha YN, Lewis GP: Structure and properties of members of the hGH family: a review. Endocr J 2000; 47(suppl):S1-S8. (Pubitemid 30243424)
23. Muller EE, Locatelli V, Cocchi D: Neuroendocrine control of growth hormone secretion. Physiol Rev 1999; 79: 511-607. (Pubitemid 29210930)
24. Bidlingmaier M, Wu Z, Strasburger CJ: Test method: GH. Baillieres Best Pract Res Clin Endocrinol Metab 2000; 14: 99-109. (Pubitemid 30487653)
25. Wallace JD, Cuneo RC, Bidlingmaier M, Lundberg PA, Carlsson L, Boguszewski CL, Hay J, Boroujerdi M, Cittadini A, Dall R, Rosén T, Strasburger CJ: Changes in non-22-kilodalton (kDa) isoforms of growth hormone (GH) after administration of 22-kDa recombinant human GH in trained adult males. J Clin Endocrinol Metab 2001; 86: 1731-1737. (Pubitemid 32374930)
26. Baxter RC: Insulin-like growth factor binding proteins in the human circulation: a review. Horm Res 1994; 42: 140-144. (Pubitemid 24336141)
27. Ohlsson C, Bengtsson BA, Isaksson OG, Andreassen TT, Slootweg MC: Growth hormone and bone. Endocr Rev 1998; 19: 55-79. (Pubitemid 29122074)
28. Seibel MJ: Molecular markers of bone turnover: biochemical, technical and analytical aspects. Osteoporos Int 2000; 11(suppl 6): S18-S29. (Pubitemid 32039510)
29. McHugh CM, Park RT, Sönksen PH, Holt RI: Challenges in detecting the abuse of growth hormone in sport. Clin Chem 2005; 51: 1587-1593. (Pubitemid 41233091)
30. Dall R, Longobardi S, Ehrnborg C, Keay N, Rosén T, Jorgensen JO, Cuneo RC, Boroujerdi MA, Cittadini A, Napoli R, Christiansen JS, Bengtsson BA, Sacca L, Baxter RC, Basset EE, Sönksen PH: The effect of four weeks of supraphysiological growth hormone administration on the insulin-like growth factor axis in women and men. GH-2000 Study Group. J Clin Endocrinol Metab 2000; 85: 4193-4200. (Pubitemid 32055372)
31. Powrie JK, Bassett EE, Rosén T, Jorgensen JO, Napoli R, Sacca L, Christiansen JS, Bengtsson BA, Sönksen PH: Detection of growth hormone abuse in sport. Growth Horm IGF Res 2007; 17: 220-226. (Pubitemid 46900098)
32. Wu Z, Bidlingmaier M, Dall R, Strasburger CJ: Detection of doping with human growth hormone. Lancet 1999; 353: 895. (Pubitemid 29126879)
33. Bidlingmaier M, Suhr J, Ernst A, Wu Z, Keller A, Strasburger CJ, Bergmann A: Highsensitivity chemiluminescence immunoassays for detection of growth hormone doping in sports. Clin Chem 2009; 55: 445-453.
34. Hashimoto Y, Ikeda I, Ikeda M, Takahashi Y, Hosaka M, Uchida H, Kono N, Fukui H, Makino T, Honjo M: Construction of a specific and sensitive sandwich enzyme immunoassay for 20 kDa human growth hormone. J Immunol Methods 1998; 221: 77-85. (Pubitemid 29017924)
35. Tsushima T, Katoh Y, Miyachi Y, Chihara K, Teramoto A, Irie M, Hashimoto Y: Serum concentration of 20K human growth hormone (20K hGH) measured by a specific enzyme-linked immunosorbent assay. Study Group of 20K hGH. J Clin Endocrinol Metab 1999; 84: 317-322. (Pubitemid 29036719)
36. Nelson AE, Ueki M, Nguyen TV, Leung KC, Howe CJ, Trout GJ, Irie M, Baxter RC, Handelsman DJ, Kazlauskas R, Ho KK: The influence of demographic factors on the ratio of 20-kDa and 22-kDa GH isoforms and the utility of the ratio for detection of GH doping in sport. Growth Horm IGF Res 2006; 16(suppl B):S3.
37. Sönksen PH: Insulin, growth hormone and sport. J Endocrinol 2001; 170: 13-25. (Pubitemid 32677562)
38. Wallace JD, Cuneo RC, Baxter R, Orskov H, Keay N, Pentecost C, Dall R, Rosén T, Jorgensen JO, Cittadini A, Longobardi S, Sacca L, Christiansen JS, Bengtsson BA, Sönksen PH: Responses of the growth hormone (GH) and insulin-like growth factor axis to exercise, GH administration, and GH withdrawal in trained adult males: a potential test for GH abuse in sport. J Clin Endocrinol Metab 1999; 84: 3591-3601. (Pubitemid 30645211)
39. Wallace JD, Cuneo RC, Lundberg PA, Rosén T, Jorgensen JO, Longobardi S, Keay N, Sacca L, Christiansen JS, Bengtsson BA, Sönksen PH: Responses of markers of bone and collagen turnover to exercise, growth hormone (GH) administration, and GH withdrawal in trained adult males. J Clin Endocrinol Metab 2000; 85: 124-133. (Pubitemid 32268801)
40. Longobardi S, Keay N, Ehrnborg C, Cittadini A, Rosén T, Dall R, Boroujerdi MA, Bassett EE, Healy ML, Pentecost C, Wallace JD, Powrie J, Jorgensen JO, Saccà L: Growth hormone (GH) effects on bone and collagen turnover in healthy adults and its potential as a marker of GH abuse in sports: a double blind, placebo-controlled study. The GH-2000. Study Group. J Clin Endocrinol Metab 2000; 85: 1505-1512. (Pubitemid 32293560)
41. Kniess A, Ziegler E, Kratzsch J, Thieme D, Müller RK: Potential parameters for the detection of hGH doping. Anal Bioanal Chem 2003; 376: 696-700. (Pubitemid 40877982)
42. Sartorio A, Agosti F, Marazzi N, Maffiuletti NA, Cella SG, Rigamonti AE, Guidetti L, Di Luigi L, Muller EE: Combined evaluation of resting IGF-I, N-terminal propeptide of type III procollagen (PIIINP) and C-terminal cross-linked telopeptide of type I collagen (ICTP) levels might be useful for detecting inappropriate GH administration in athletes: a preliminary report. Clin Endocrinol (Oxf) 2004; 61: 487-493. (Pubitemid 39388488)
43. Ehrnborg C, Ohlsson C, Mohan S, Bengtsson BA, Rosén T: Increased serum concentration of IGFBP-4 and IGFBP-5 in healthy adults during one month's treatment with supraphysiological doses of growth hormone. Growth Horm IGF Res 2007; 17: 234-241. (Pubitemid 46900099)
44. Nelson AE, Meinhardt U, Hansen JL, Walker IH, Stone G, Howe CJ, Leung KC, Seibel MJ, Baxter RC, Handelsman DJ, Kazlauskas R, Ho KK: Pharmacodynamics of GH abuse biomarkers and the influence of gender and testosterone: a randomized double-blind placebo-controlled study in young recreational athletes. J Clin Endocrinol Metab 2008; 93: 2213-2222. (Pubitemid 351831538)
45. Nelson AE, Howe CJ, Nguyen TV, Leung KC, Trout GJ, Seibel MJ, Baxter RC, Handelsman DJ, Kazlauskas R, Ho KK: Influence of demographic factors and sport type on growth hormone-responsive markers in elite athletes. J Clin Endocrinol Metab 2006; 91: 4424-4432. (Pubitemid 44833418)
46. Juul A, Dalgaard P, Blum WF, Bang P, Hall K, Michaelsen KF, Müller J, Skakkebaek NE: Serum levels of insulin-like growth factor (IGF)-binding protein-3 (IGFBP-3) in healthy infants, children, and adolescents: the relation to IGF-I, IGF-II, IGFBP-1, IGFBP-2, age, sex, body mass index, and pubertal maturation. J Clin Endocrinol Metab 1995; 80: 2534-2542.
47. Szulc P, Seeman E, Delmas PD: Biochemical measurements of bone turnover in children and adolescents. Osteoporos Int 2000; 11: 281-294. (Pubitemid 30417180)
48. Healy ML, Dall R, Gibney J, Bassett E, Ehrnborg C, Pentecost C, Rosén T, Cittadini A, Baxter RC, Sönksen PH: Toward the development of a test for growth hormone (GH) abuse: a study of extreme physiological ranges of GH-dependent markers in 813 elite athletes in the postcompetition setting. J Clin Endocrinol Metab 2005; 90: 641-649. (Pubitemid 40279174)
49. Nguyen TV, Nelson AE, Howe CJ, Seibel MJ, Baxter RC, Handelsman DJ, Kazlauskas R, Ho KK: Within-subject variability and analytic imprecision of insulinlike growth factor axis and collagen markers: implications for clinical diagnosis and doping tests. Clin Chem 2008; 54: 1268-1276.
50. Kurdy NM, Bowles S, Marsh DR, Davies A, France M: Serology of collagen types I and III in normal healing of tibial shaft fractures. J Orthop Trauma 1998; 12: 122-126. (Pubitemid 128452800)
51. Stoffel K, Engler H, Kuster M, Riesen W: Changes in biochemical markers after lower limb fractures. Clin Chem 2007; 53: 131-134. (Pubitemid 46047773)
52. Erotokritou-Mulligan I, Bassett EE, Bartlett C, Cowan D, McHugh C, Seah R, Curtis B, Wells V, Harrison K, Sönksen PH, Holt RI: The effect of sports injury on insulin-like growth factor-I and type 3 procollagen: implications for detection of growth hormone abuse in athletes. J Clin Endocrinol Metab 2008; 93: 2760-2763. (Pubitemid 352008548)
53. Erotokritou-Mulligan I, Bassett EE, Kniess A, Sönksen PH, Holt RI: Validation of the growth hormone (GH)-dependent marker method of detecting GH abuse in sport through the use of independent data sets. Growth Horm IGF Res 2007; 17: 416-423. (Pubitemid 47324307)
54. Nelson AE, Ho KK: A robust test for growth hormone doping-present status and future prospects. Asian J Androl 2008; 10: 416-425. (Pubitemid 351584117)