External calibration in Gas Chromatography-Combustion-Isotope Ratio Mass Spectrometry measurements of endogenous androgenic anabolic steroids in sports doping control

Journal of Chromatography A

Volumn 1218, Issue 33, 2011, Pages 5675-5682

  Kioussi, Maroula K ^a,b   Angelis, Yiannis S ^a   Cawley, Adam T ^c   Koupparis, Michalis ^b   Kazlauskas, Rymantas ^c   Brenna, J Thomas ^d   Georgakopoulos, Costas G ^a  

^a Doping Control Laboratory of Athens   (Greece)

^b UNIVERSITY OF ATHENS   (Greece)

^c NATIONAL MEASUREMENT INSTITUTE   (Australia)

^d Department of Obstetrics Gynecology   (United States)

Author keywords

Doping control; Endogenous steroids; External calibration; Gas Chromatography Combustion Isotope Ratio Mass Spectrometry (GC C IRMS); Interlaboratory study

Indexed keywords

ACCREDITED LABORATORIES; ANDROGENIC ANABOLIC STEROIDS; ATHENS , GREECE; CALIBRATION CURVES; CALIBRATION MIXTURES; CALIBRATION PROCEDURE; CALIBRATION STANDARD; CHROMATOGRAPHIC RETENTION; DOPING CONTROL; ENDOGENOUS STEROIDS; ETIOCHOLANOLONE; EXTERNAL CALIBRATION; INTERLABORATORY STUDIES; ISOTOPIC COMPOSITION; LEAST SQUARES FIT; MIXTURE SOLUTION; SPECTROMETRY MEASUREMENTS; SYDNEY , AUSTRALIA; URINE SAMPLE; WORLD ANTI-DOPING AGENCY;

CALIBRATION; CARBON DIOXIDE; COMBUSTION; ISOTOPES; LABORATORIES; LIPIDS; MASS SPECTROMETRY; SPECTROMETERS; STANDARDS;

GAS CHROMATOGRAPHY;

5BETA ANDROSTANE 3ALPHA 17BETA DIACETATE; ANDROGEN; ANDROSTERONE; CARBON DIOXIDE; ETIOCHOLANOLONE; ISOTOPE; PREGNANEDIACETATE; UNCLASSIFIED DRUG;

ARTICLE; AUSTRALIA; CALIBRATION; CHROMATOGRAPHY; DIAGNOSTIC ACCURACY; DOPING; GAS CHROMATOGRAPHY; GAS CHROMATOGRAPHY COMBUSTION ISOTOPE RATIO MASS SPECTROMETRY; GREECE; HORMONE DETERMINATION; LABORATORY; MASS SPECTROMETRY; PRIORITY JOURNAL; SIGNAL TRANSDUCTION; SPORT; STEROID URINE LEVEL; URINALYSIS;

ANABOLIC AGENTS; CALIBRATION; CARBON ISOTOPES; DOPING IN SPORTS; GAS CHROMATOGRAPHY-MASS SPECTROMETRY; HUMANS; MALE; STEROIDS; SUBSTANCE ABUSE DETECTION;

EID: 79960696792     PISSN: 00219673     EISSN: 18733778     Source Type: Journal    
DOI: 10.1016/j.chroma.2011.06.014     Document Type: Article

References (45)

1. World Anti-Doping Agency, WADA Laboratory Statistics, 2009 Adverse Analytical Findings Reported by Accredited Laboratories, available: (accessed March 2011). http://www.wada-ama.org/Documents/Science_Medicine/Anti-Doping_Laboratories/Lab_Statistics/WADA_2009_LaboratoryStatisticsReport_Final.pdf.
2. Sottas P.-E., Baume N., Saudan C., Schweizer C., Kamber M., Saugy M. Biostatistics 2007, 8:285.
3. Sottas P.-E., Saudan C., Schweizer C., Baume N., Mangin P., Saugy M. Forensic Sci. Int. 2008, 174:166.
4. Becchi M., Aguilera R., Farizon Y., Flament M.-M., Casabianca H., James P. Rapid Commun. Mass Spectrom. 1994, 8:304.
5. Shackleton C.H.L., Phillips A., Chang T., Li Y. Steroids 1997, 62:379.
6. Shackleton C.H.L., Roitman E., Phillips A., Chang T. Steroids 1997, 62:665.
7. Aguilera R., Chapman T.E., Starcevic B., Hatton C.K., Catlin D.H. Clin. Chem. 2001, 47:292.
8. Saudan C., Kamber M., Barbati G., Robinson N., Desmarchelier A., Mangin P., Saugy M. J. Chromatogr. B 2006, 831:324.
9. Aguilera R., Becchi M., Grenot C., Casabianca H., Hatton C.K. J. Chromatogr. B: Biomed. Appl. 1996, 687:43.
10. Aguilera R., Becchi M., Casabianca H., Hatton C.K., Catlin D.H., Starcevic B., Pope H.G. J. Mass Spectrom. 1996, 31:169.
11. Horning S., Geyer H., Flenker U., Schanzer W. Recent Advances in Doping Analysis 1997, 135. Sport and Buch Strauβ, Cologne. 5th ed. W. Schanzer, H. Geyer, A. Goltmann, U. Marek-Engelke (Eds.).
12. Aguilera R., Catlin D.H., Becchi M., Phillips A., Wang C., Swerdloff R.S., Pope H.G., Hatton C.K. J. Chromatogr. B: Biomed. Sci. Appl. 1999, 727:95.
13. Ueki M., Okano M. J. Toxicol. Toxin Rev. 1999, 18:177.
14. Segura J., Peng S.H., de la Torre X. J. Toxicol. Toxin Rev. 1999, 18:125.
15. Aguilera R., Chapman T.E., Catlin D.H. Rapid Commun. Mass Spectrom. 2000, 14:2294.
16. Piper T., Mareck U., Geyer H., Flenker U., Thevis M., Platen P., Schänzer W. Rapid Commun. Mass Spectrom. 2008, 22:2161.
17. Cawley A.T., Flenker U. J. Mass Spectrom. 2008, 43:854.
18. Piper T., Geyer H., Gougoulidis V., Flenker U., Schanzer W. Drug Test. Anal. 2010, 2:217.
19. de la Torre X., Gonzales J.C., Pichini S., Pascual J.A., Segura J. J. Pharm. Biomed. Anal. 2001, 24:645.
20. Piper T., Flenker U., Mareck U., Schanzer W. Drug Test. Anal. 2009, 1:65.
21. Saudan C., Baume N., Mangin P., Saugy M. J. Chromatogr. B 2004, 810:157.
22. Meier-Augenstein W. LC-GC Int. 1997, 15:244.
23. Meier-Augenstein W., Liu R.H. Advances in Forensic Applications of Mass Spectrometry 2004, CRC Press LLC. J. Yinon (Ed.).
24. Craig H. Geochim. Cosmochim. Acta 1957, 12:133.
25. Brand W.A. J. Mass Spectrom. 1996, 31:225.
26. Merritt D.A., Brand W.A., Hayes J.M. Org. Geochem. 1994, 21:573.
27. Caimi R.J., Houghton L.H., Brenna J.T. Anal. Chem. 1994, 66:2989.
28. Caimi R.J., Brenna J.T. J. Am. Soc. Mass Spectrom. 1996, 7:605.
29. Meier-Augenstein W., Watt P.W., Langhans C.-D. J. Chromatogr. A 1996, 752:233.
30. Meier-Augenstein W. Rapid Commun. Mass Spectrom. 1997, 11:1775.
31. Meier-Augenstein W. J. Chromatogr. A 1999, 842:351.
32. Zhang Y., Tobias H.J., Brenna J.T. Steroids 2009, 74:369.
33. Ueki M., Okano M. Rapid Commun. Mass Spectrom. 1999, 13:2237.
34. Flenker U., Güntner U., Schänzer W. Steroids 2008, 73:408.
35. Munton E., Murby J., Hibbert D.B., Santamaria-Ferrnandez R. Rapid Commun. Mass Spectrom. 2011, 25:1614.
36. WADA Laboratory Committee, Reporting and Evaluation Guidance for Testosterone, Epitestosterone, T/E Ratio and Other Endogenous Steroids, World Anti-Doping Agency: Montreal, 2004, WADA Technical Document - TD2004EAAS.
37. Silfer J.A., Engel M.H., Macko S.A. Anal. Chem. 1991, 63:370.
38. Rieley G. Analyst 1994, 119:915.
39. Macko S.A., Ryan M., Engel M.H. Chem. Geol. 1998, 152:205.
40. Docherty G., Jones V., Evershed R.P. Rapid Commun. Mass Spectrom. 2001, 15:730.
41. World Anti-Doping Agency, International Standard for Laboratories, 2009, available: (accessed March 2011). http://www.wada-ama.org/Documents/World_Anti-Doping_%20Program/WADP-IS-Laboratories/WADA_Int.Standard_Laboratories_2009_EN.pdf.
42. Werner R.A., Brand W.A. Rapid Commun. Mass Spectrom. 2001, 15:501.
43. Santrock J., Studley S.A., Hayes J.M. Anal. Chem. 1985, 57:7444.
44. Thorne G.C., Gaskell S.J., Payne P.A. Biomed. Mass Spectrom. 1984, 11:415.
45. Norlin E., Irgum K., Anders Ohlsson K.E. Analyst 2002, 127:735.