Changes in glucose uptake rate and in the energy status of PC-12 cells acutely exposed to hexavalent chromium, an established human carcinogen

Toxicological and Environmental Chemistry

Volumn 93, Issue 6, 2011, Pages 1202-1211

  Gonçalves, Maria João ^a   Santos, Ana Catarina Calado ^a   Rodrigues, Carlos Fernando Dias ^a   Coelho, Pedro ^a   Costa, André Nuno ^a   Guiomar, António Jorge ^a,c   Santos, Maria Sancha ^a,b   Alpoim, Maria Carmen ^a,b,d   Urbano, Ana Margarida ^a,d,e  

^a UNIVERSITY OF COIMBRA   (Portugal)

^b UNIVERSITY OF COIMBRA   (Portugal)

^c UNIVERSITY OF COIMBRA   (Portugal)

^d UNIVERSITY OF COIMBRA   (Portugal)

^e UNIVERSITY OF COIMBRA   (Portugal)

Author keywords

Chromate induced carcinogenesis; Energy charge; Lactate production; Oxygen consumption

Indexed keywords

CANCER CELLS; CELLULAR FUNCTION; CHROMATE-INDUCED CARCINOGENESIS; CONCENTRATION-DEPENDENT; ENERGY CHARGE; ENERGY METABOLISM; ENERGY PRODUCTIONS; ENERGY STATUS; ENHANCED UPTAKE; GLUCOSE UPTAKE; HEXAVALENT CHROMIUM; HUMAN CARCINOGEN; LACTATE PRODUCTION; METABOLIC SHIFT; OXYGEN CONSUMPTION; PHYSIOLOGICAL STATE; SOLID TUMORS;

CHROMATES; CHROMIUM; DISEASES; GLUCOSE; METABOLISM; OXYGEN; PATHOLOGY; PHYSIOLOGICAL MODELS; PHYSIOLOGY;

ELECTROMAGNETIC INDUCTION;

BIOENERGETICS; BIOLOGICAL UPTAKE; CANCER; CARCINOGEN; CHROMIUM; CONCENTRATION (COMPOSITION); CYTOLOGY; FERMENTATION; GLUCOSE; METABOLISM; OXYGEN CONSUMPTION; PHENOTYPE; PHYSIOLOGY; POLLUTION EXPOSURE; PUBLIC HEALTH; TUMOR;

EID: 79960665821     PISSN: 02772248     EISSN: 10290486     Source Type: Journal    
DOI: 10.1080/02772248.2011.581340     Document Type: Article

References (34)

1. Andreoli, T., B. Daldegan, I. Deangelis, E. Fortunati, D. Reggiani, V. Bianchi, R. Tiozzo, and F. Zucco. 1991. Evaluation of metabolic end-points of acute cytotoxicity in V79-fibroblasts. Toxicology In Vitro 5, no. 5-6: 549-53.
2. Atkinson, D.E. 1968. Energy charge of the adenylate pool as a regulatory parameter. Interaction with feedback modifiers. Biochemistry 7, no. 11: 4030-4.
3. Bianchi, V., P. Debetto, A. Zantedeschi, and A.G. Levis. 1982. Effects of hexavalent chromium on the adenylate pool of hamster fibroblasts. Toxicology 25, no. 1: 19-30.
4. Bradford, M.M. 1976. A rapid and sensitive method for the quantitation of microgram quantities of protein utilizing the principle of protein-dye binding. Analytical Biochemistry 72, no. 1-2: 248-54.
5. Carmichael, J., W.G. Degraff, A.F. Gazdar, J.D. Minna, and J.B. Mitchell. 1987. Evaluation of a tetrazolium-based semi-automated colorimetric assay-Assessment of chemosensitivity testing. Cancer Research 47, no. 4: 936-42.
6. Costa, A.N., V. Moreno, M.J. Prieto, A.M. Urbano, and M.C. Alpoim. 2010. Induction of morphological changes in BEAS-2B human bronchial epithelial cells following chronic subcytotoxic and mildly cytotoxic hexavalent chromium exposures. Molecular Carcinogenesis 49, no. 6: 582-91.
7. Czernin, J., and M.E. Phelps. 2002. Positron emission tomography scanning: Current and future applications. Annual Review of Medicine 53: 89-112.
8. Debetto, P., R.D. Toso, R. Varotto, V. Bianchi, and S. Luciani. 1982. Effects of potassium dichromate on ATP content of mammalian cells cultured in vitro. Chemico-Biological Interactions 41, no. 1: 15-24.
9. Fernandes, M.A.S., M.S. Santos, M.C. Alpoim, V.M.C. Madeira, and J.A.F. Vicente. 2002. Chromium(VI) interaction with plant and animal mitochondrial bioenergetics: A comparative study. Journal of Biochemical and Molecular Toxicology 16, no. 2: 53-63.
10. Hanahan, D., and R.A. Weinberg. 2011. Hallmarks of cancer: The next generation. Cell 144, no. 5: 646-74.
11. Hayashi, T., M.F. Hirshman, E.J. Kurth, W.W. Winder, and L.J. Goodyear. 1998. Evidence for 5' AMP-activated protein kinase mediation of the effect of muscle contraction on glucose transport. Diabetes 47, no. 8: 1369-73.
12. Holmes, A.L., S.S. Wise, S.J. Sandwick, and J.P. Wise. 2006. The clastogenic effects of chronic exposure to particulate and soluble Cr(VI) in human lung cells. Mutation Research-Genetic Toxicology and Environmental Mutagenesis 610, no. 1-2: 8-13.
13. IARC, I.A.F.R.O.C. 1990. Chromium, nickel and welding. Vol. 49 of IARC monographs on the evaluation of carcinogenic risks to humans. Lyon: IARC Scientific Publications.
14. Kahn, B.B., T. Alquier, D. Carling, and D.G. Hardie. 2005. Amp-activated protein kinase: Ancient energy gauge provides clues to modern understanding of metabolism. Cell Metabolism 1, no. 1: 15-25.
15. Lazzarini, A., S. Luciani, M. Beltrame, and P. Arslan. 1985. Effects of chromium(VI) and chromium(III) on energy-charge and oxygen-consumption in rat thymocytes. Chemico-Biological Interactions 53, no. 3: 273-81.
16. Lessler, M.A., and G.P. Brierley. 1969. Oxygen electrode measurements in biochemical analysis. Methods of Biochemical Analysis 17: 1-29.
17. Liu, W., F. Chaspoul, C. Botta, M. De Meo, and P. Gallice. 2010. Bioenergetics and DNA alteration of normal human fibroblasts by hexavalent chromium. Environmental Toxicology and Pharmacology 29, no. 1: 58-63.
18. Messer, R.L.W., J.E. Doeller, D.W. Kraus, and L.C. Lucas. 2000. An investigation of fibroblast mitochondria enzyme activity and respiration in response to metallic ions released from dental alloys. Journal of Biomedical Materials Research 50, no. 4: 598-604.
19. Noll, F. 1984. L-(+)-lactate. In Methods of enzymatic analysis, ed. H.U. Bergmeyer, 582-8. Weinheim: Verlag Chemie.
20. O'Brien, T.J., S. Ceryak, and S.R. Patierno. 2003. Complexities of chromium carcinogenesis: Role of cellular response, repair and recovery mechanisms. Mutation Research-Fundamental and Molecular Mechanisms of Mutagenesis 533, no. 1-2: 3-36.
21. Patierno, S.R., D. Banh, and J.R. Landolph. 1988. Transformation of c3h-10t1/2 mouse embryo cells to focus formation and anchorage independence by insoluble lead chromate but not soluble calcium chromate: Relationship to mutagenesis and internalization of lead chromate particles. Cancer Research 48, no. 18: 5280-88.
22. Pedersen, P.L. 2007. The cancer cell's "Power plants" as promising therapeutic targets: An overview. Journal of Bioenergetics and Biomembranes 39, no. 1: 1-12.
23. Rodrigues, C.F.D., A.M. Urbano, E. Matoso, I. Carreira, A. Almeida, P. Santos, F. Botelho, et al. 2009. Human bronchial epithelial cells malignantly transformed by hexavalent chromium exhibit an aneuploid phenotype but no microsatellite instability. Mutation Research-Fundamental and Molecular Mechanisms of Mutagenesis 670, no. 1-2: 42-52.
24. Rossi, S.C., N. Gorman, and K.E. Wetterhahn. 1988. Mitochondrial reduction of the carcinogen chromate: Formation of chromium(V). Chemical Research in Toxicology 1, no. 2: 101-7.
25. Rossi, S.C., and K.E. Wetterhahn. 1989. Chromium(V) is produced upon reduction of chromate by mitochondrial electron transport chain complexes. Carcinogenesis 10, no. 5: 913-20.
26. Ryberg, D., and J. Alexander. 1984. Inhibitory action of hexavalent chromium (Cr(VI) on the mitochondrial respiration and a possible coupling to the reduction of Cr(VI). Biochemical Pharmacology 33, no. 15: 2461-6.
27. Ryberg, D., and J. Alexander. 1990. Mechanisms of chromium toxicity in mitochondria. Chemico-Biological Interactions 75, no. 2: 141-51.
28. Shi, X., A. Chiu, C.T. Chen, B. Halliwell, V. Castranova, and V. Vallyathan. 1999. Reduction of chromium VI and its relationship to carcinogenesis. Journal of Toxicology and Environmental Health Part B 2, no. 1: 87-104.
29. Stearns, D.M., and K.E. Wetterhahn. 1994. Reaction of chromium(VI) with ascorbate produces chromium(v), chromium(iv), and carbon-based radicals. Chemical Research in Toxicology 7, no. 2: 219-30.
30. Stocchi, V., L. Cucchiarini, M. Magnani, L. Chiarantini, P. Palma, and G. Crescentini. 1985. Simultaneous extraction and reverse-phase high-performance liquid chromatographic determination of adenine and pyridine nucleotides in human red blood cells. Analytical Biochemistry 146, no. 1: 118-24.
31. Trinder, P. 1969. Determination of glucose in the blood using glucose oxidase and an alternative oxygen acceptor. Annals of Clinical Biochemistry 6: 24-7.
32. Urbano, A.M., C.F.D. Rodrigues, and M.C. Alpoim. 2008. Hexavalent chromium exposure, genomic instability and lung cancer. Gene Therapy and Molecular Biology 12B: 219-38.
33. Warburg, O. 1930. The metabolism of tumors. London: Constable & Co. Ltd.
34. Zhitkovich, A. 2005. Importance of chromium-DNA adducts in mutagenicity and toxicity of chromium (VI). Chemical Research in Toxicology 18, no. 1: 3-11.