Genotyping of the protozoan pathogen Toxoplasma gondii using high-resolution melting analysis of the repeated B1 gene

Journal of Microbiological Methods

Volumn 86, Issue 3, 2011, Pages 357-363

  Costa, Jean Marc ^a,b   Cabaret, Odile ^a,c   Moukoury, Sandrine ^a   Bretagne, Stéphane ^a,c  

^a HENRI MONDOR HOSPITAL   (France)

^b LABORATOIRE CERBA   (France)

^c UNIVERSITÉ PARIS EST CRÉTEIL   (France)

Author keywords

B1 gene; Genotyping; High Resolution Melting Analyses; Toxoplasma gondii

Indexed keywords

ARTICLE; B1 GENE; CONTROLLED STUDY; GENE; GENE AMPLIFICATION; GENE LOCUS; GENETIC ANALYSIS; GENETIC VARIABILITY; GENOTYPE; HIGH RESOLUTION MELTING ANALYSIS; MICROBIOLOGICAL EXAMINATION; MICROSATELLITE MARKER; NONHUMAN; NUCLEOTIDE SEQUENCE; PRIORITY JOURNAL; QUANTITATIVE ANALYSIS; SEQUENCE ANALYSIS; SINGLE NUCLEOTIDE POLYMORPHISM; STRAIN DIFFERENCE; TOXOPLASMA GONDII;

BASE SEQUENCE; DNA PRIMERS; DNA, PROTOZOAN; GENOME, PROTOZOAN; GENOTYPE; MICROSATELLITE REPEATS; NUCLEIC ACID DENATURATION; POLYMERASE CHAIN REACTION; POLYMORPHISM, SINGLE NUCLEOTIDE; SEQUENCE ANALYSIS, DNA; TOXOPLASMA;

PROTOZOA; TOXOPLASMA GONDII;

EID: 79960645068     PISSN: 01677012     EISSN: 18728359     Source Type: Journal    
DOI: 10.1016/j.mimet.2011.06.017     Document Type: Article

References (17)

1. Ajzenberg D., Dumetre A., Darde M.L. Multiplex PCR for typing strains of Toxoplasma gondii. J. Clin. Microbiol. 2005, 43:1940-1943.
2. Ajzenberg D., Collinet F., Mercier A., Vignoles P., Darde M.L. Genotyping of Toxoplasma gondii isolates with 15 microsatellite markers in a single multiplex PCR assay. J. Clin. Microbiol. 2010, 48:4641-4645.
3. Bretagne S., Costa J.M., Vidaud M., Tran J., Nhieu V., Fleury-Feith J. Detection of Toxoplasma gondii by competitive DNA amplification of bronchoalveolar lavage samples. J. Infect. Dis. 1993, 168:1585-1588.
4. Costa J.M., Darde M.L., Assouline B., Vidaud M., Bretagne S. Microsatellite in the beta-tubulin gene of Toxoplasma gondii as a new genetic marker for use in direct screening of amniotic fluids. J. Clin. Microbiol. 1997, 35:2542-2545.
5. Costa J.M., Garcia-Hermoso D., Olivi M., Cabaret O., Farrugia C., Lecellier G., et al. Genotyping of Candida albicans using length fragment and high-resolution melting analyses together with minisequencing of a polymorphic microsatellite locus. J. Microbiol. Methods 2010, 80:306-309.
6. Di Fiore F., Blanchard F., Charbonnier F., Le Pessot F., Lamy A., Galais M.P., et al. Clinical relevance of KRAS mutation detection in metastatic colorectal cancer treated by Cetuximab plus chemotherapy. Br. J. Cancer 2007, 96:1166-1169.
7. Grigg M.E., Boothroyd J.C. Rapid identification of virulent type I strains of the protozoan pathogen Toxoplasma gondii by PCR-restriction fragment length polymorphism analysis at the B1 gene. J. Clin. Microbiol. 2001, 39:398-400.
8. Hohlfeld P., Daffos F., Costa J.M., Thulliez P., Forestier F., Vidaud M. Prenatal diagnosis of congenital toxoplasmosis with a polymerase-chain-reaction test on amniotic fluid. N. Engl. J. Med. 1994, 331:695-699.
9. Howe D.K., Sibley L.D. Toxoplasma gondii comprises three clonal lineages: correlation of parasite genotype with human disease. J. Infect. Dis. 1995, 172:1561-1566.
10. Hurst C.D., Zuiverloon T.C., Hafner C., Zwarthoff E.C., Knowles M.A. A SNaPshot assay for the rapid and simple detection of four common hotspot codon mutations in the PIK3CA gene. BMC Res. Notes 2009, 2:66.
11. Reed G.H., Kent J.O., Wittwer C.T. High-resolution DNA melting analysis for simple and efficient molecular diagnostics. Pharmacogenomics 2007, 8:597-608.
12. Reischl U., Bretagne S., Kruger D., Ernault P., Costa J.M. Comparison of two DNA targets for the diagnosis of toxoplasmosis by real-time PCR using fluorescence resonance energy transfer hybridization probes. BMC Infect. Dis. 2003, 3:7.
13. Saeij J.P., Boyle J.P., Boothroyd J.C. Differences among the three major strains of Toxoplasma gondii and their specific interactions with the infected host. Trends Parasitol. 2005, 21:476-481.
14. Sibley L.D., Boothroyd J.C. Virulent strains of Toxoplasma gondii comprise a single clonal lineage. Nature 1992, 359:82-85.
15. Su C., Shwab E.K., Zhou P., Zhu X.Q., Dubey J.P. Moving towards an integrated approach to molecular detection and identification of Toxoplasma gondii. Parasitology 2010, 137:1-11.
16. Switaj K., Master A., Borkowski P.K., Skrzypczak M., Wojciechowicz J., Zaborowski P. Association of ocular toxoplasmosis with type I Toxoplasma gondii strains: direct genotyping from peripheral blood samples. J. Clin. Microbiol. 2006, 44:4262-4264.
17. Wittwer C.T., Reed G.H., Gundry C.N., Vandersteen J.G., Pryor R.J. High-resolution genotyping by amplicon melting analysis using LCGreen. Clin. Chem. 2003, 49:853-860.