Construction and evaluation of pMycoFos, a fosmid shuttle vector for Mycobacterium spp. with inducible gene expression and copy number control

Journal of Microbiological Methods

Volumn 86, Issue 3, 2011, Pages 320-326

  Ly, Mai Anh ^a   Liew, Elissa F ^a   Le, Nga B ^a   Coleman, Nicholas V ^a  

^a UNIVERSITY OF SYDNEY   (Australia)

Author keywords

Cloning vector; Fosmid; Gene expression; Mycobacterium

Indexed keywords

ACETAMIDE; ARABINOSE; BACTERIAL DNA; BETA GALACTOSIDASE; CYTOSINE; ETHENE MONOOXYGENASE; GUANINE; UNCLASSIFIED DRUG; UNSPECIFIC MONOOXYGENASE;

ARTICLE; BACTERIAL GENE; BACTERIUM CULTURE; CONTROLLED STUDY; DNA CONTENT; ENZYME ACTIVITY; ESCHERICHIA COLI; GENE CLUSTER; GENE DOSAGE; GENE EXPRESSION; GENE INSERTION; HETEROLOGOUS EXPRESSION; LACZ GENE; MOLECULAR CLONING; MYCOBACTERIUM; MYCOBACTERIUM SMEGMATIS; NONHUMAN; NUCLEOTIDE SEQUENCE; POLYACRYLAMIDE GEL ELECTROPHORESIS; PRIORITY JOURNAL; PROTEIN ANALYSIS; SHUTTLE VECTOR;

BETA-GALACTOSIDASE; CLONING, MOLECULAR; DNA COPY NUMBER VARIATIONS; GENE EXPRESSION; GENE TRANSFER TECHNIQUES; GENETIC VECTORS; LAC OPERON; MYCOBACTERIUM; PROTEIN ARRAY ANALYSIS;

MYCOBACTERIUM; MYCOBACTERIUM SMEGMATIS; NEGIBACTERIA; POSIBACTERIA;

EID: 79960640458     PISSN: 01677012     EISSN: 18728359     Source Type: Journal    
DOI: 10.1016/j.mimet.2011.06.005     Document Type: Article

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