Nuclear-cytoplasmic incompatibility and inefficient development of pig-mouse cytoplasmic hybrid embryos

Reproduction

Volumn 142, Issue 2, 2011, Pages 295-307

  Amarnath, Dasari ^a,b,c   Choi, Inchul ^a,d   Moawad, Adel R ^a,e   Wakayama, Teruhiko ^b   Campbell, Keith H S ^a  

^a UNIVERSITY OF NOTTINGHAM   (United Kingdom)

^b Center for Developmental Biology   (Japan)

^c TEMPLE UNIVERSITY SCHOOL OF MEDICINE   (United States)

^d MICHIGAN STATE UNIVERSITY   (United States)

^e Cairo University   (Egypt)

Author keywords

[No Author keywords available]

Indexed keywords

CHORIONIC GONADOTROPIN; CYTOCHROME C OXIDASE; CYTOCHROME C OXIDASE 1; CYTOCHROME C OXIDASE 2; DNA DIRECTED DNA POLYMERASE GAMMA; DNA POLYMERASE GAMMA 2; GLUCOSE TRANSPORTER 1; GLUCOSE TRANSPORTER 3; HEAT SHOCK PROTEIN 27; MITOCHONDRIAL DNA; MITOCHONDRIAL TRANSCRIPTION FACTOR A; MITOFUSIN 2; OCTAMER TRANSCRIPTION FACTOR 4; PROTEIN BCL 2; TRANSCRIPTION FACTOR AP 2; TRANSCRIPTION FACTOR AP 2 GAMMA; TRANSCRIPTION FACTOR CDX2; TRANSCRIPTION FACTOR GATA 3; TRANSCRIPTION FACTOR NANOG; UNCLASSIFIED DRUG;

ANIMAL CELL; ANIMAL EXPERIMENT; ARTICLE; BLASTOCYST; CELL DIFFERENTIATION; CELL VIABILITY; CYTOPLASM; DNA METHYLATION; DNA REPLICATION; DOWN REGULATION; EMBRYO DEVELOPMENT; EMBRYO TRANSFER; FEMALE; GENE DOSAGE; GENE EXPRESSION; GENE SEQUENCE; INTERSPECIES SOMATIC CELL NUCLEAR TRANSFER; MALE; MOLECULAR CLONING; MOUSE; NONHUMAN; PRIORITY JOURNAL; PROMOTER REGION; ZYGOTE;

ANIMALS; BLASTOCYST; CATTLE; CELL FUSION; CELL NUCLEUS; CLONING, ORGANISM; CYTOPLASM; DNA, MITOCHONDRIAL; EMBRYO CULTURE TECHNIQUES; EMBRYONIC DEVELOPMENT; FEMALE; GENE EXPRESSION PROFILING; GENE EXPRESSION REGULATION, DEVELOPMENTAL; MALE; MICE; MORULA; NUCLEAR TRANSFER TECHNIQUES; SHEEP, DOMESTIC; SPECIES SPECIFICITY; SUS SCROFA; TRANSCRIPTION, GENETIC; ZYGOTE;

MURINAE; SUIDAE; SUS;

EID: 79960634454     PISSN: 14701626     EISSN: 17417899     Source Type: Journal    
DOI: 10.1530/REP-11-0044     Document Type: Article

References (80)

1. Acton BM, Jurisicova A, Jurisica I & Casper RF 2004 Alterations in mitochondrial membrane potential during preimplantation stages of mouse and human embryo development. Molecular Human Reproduction 10 23-32. (doi:10.1093/molehr/gah004)
2. Alizadeh Z, Kageyama S & Aoki F 2005 Degradation of maternal mRNA in mouse embryos: selective degradation of specific mRNAs after fertilization. Molecular Reproduction and Development 72 281-290. (doi:10.1002/mrd.20340)
3. Amarnath D, Li X, Kato Y & Tsunoda Y 2007 Gene expression in individual bovine somatic cell cloned embryos at the 8-cell and blastocyst stages of preimplantation development. Journal of Reproduction and Development 53 1247-1263. (doi:10.1262/jrd.19096)
4. Bao Q & Shi Y 2007 Apoptosome: A platform for the activation of initiator caspases. Cell Death and Differentiation 14 56-65. (doi:10.1038/sj.cdd.4402028)
5. Beyhan Z, Iager AE & Cibelli JB 2007 Interspecies nuclear transfer: implications for embryonic stem cell biology. Cell Stem Cell 1 502-512. (doi:10.1016/j.stem.2007.10.009)
6. Bian Y, Alberio R, Allegrucci C, Campbell KH & Johnson AD 2009 Epigenetic marks in somatic chromatin are remodelled to resemble pluripotent nuclei by amphibian oocyte extracts. Epigenetics 4 194-202. (doi:10.4161/epi.4. 3.8787)
7. Blakaj A & Lin H 2008 Piecing together the mosaic of early mammalian development through microRNAs. Journal of Biological Chemistry 283 9505-9508. (doi:10.1074/jbc.R800002200)
8. Bowles EJ, Tecirlioglu RT, French AJ, Holland MK & St John JC 2008 Mitochondrial DNA transmission and transcription after somatic cell fusion to one or more cytoplasts. Stem Cells 26 775-782. (doi:10.1634/stemcells.2007-0747)
9. Brill A, Torchinsky A, Carp H & Toder V 1999 The role of apoptosis in normal and abnormal embryonic development. Journal of Assisted Reproduction and Genetics 16 512-519. (doi:10.1023/A:1020541019347)
10. Campbell KH, Fisher P, Chen WC, Choi I, Kelly RD, Lee JH & Xhu J 2007 Somatic cell nuclear transfer: past, present and future perspectives. Theriogenology 68 (Supplement 1) S214-S231. (doi:10.1016/j.theriogenology.2007. 05.059)
11. Chalitchagorn K, Shuangshoti S, Hourpai N, Kongruttanachok N, Tangkijvanich P, Thong-ngam D, Voravud N, Sriuranpong V & Mutirangura A 2004 Distinctive pattern of LINE-1 methylation level in normal tissues and the association with carcinogenesis. Oncogene 23 8841-8846. (doi:10.1038/sj.onc. 1208137)
12. Chen H & Chan DC 2005 Emerging functions of mammalian mitochondrial fusion and fission. Human Molecular Genetics 14 R283-R289. (doi:10.1093/hmg/ ddi270)
13. Chen DY, Wen DC, Zhang YP, Sun QY, Han ZM, Liu ZH, Shi P, Li JS, Xiangyu JG, Lian L et al. 2002 Interspecies implantation and mitochondria fate of panda-rabbit cloned embryos. Biology of Reproduction 67 637-642. (doi:10.1095/biolreprod67.2.637)
14. Chen H, Detmer SA, Ewald AJ, Griffin EE, Fraser SE & Chan DC 2003a Mitofusins Mfn1 and Mfn2 coordinately regulate mitochondrial fusion and are essential for embryonic development. Journal of Cell Biology 160 189-200. (doi:10.1083/jcb.200211046)
15. Chen Y, He ZX, Liu A, Wang K, Mao WW, Chu JX, Lu Y, Fang ZF, Shi YT, Yang QZ et al. 2003b Embryonic stem cells generated by nuclear transfer of human somatic nuclei into rabbit oocytes. Cell Research 13 251-263. (doi:10.1038/sj.cr.7290170)
16. Cheng Y, Wang K, Kellam LD, Lee YS, Liang CG, Han Z, Mtango NR & Latham KE 2009 Effects of ooplasm manipulation on DNA methylation and growth of progeny in mice. Biology of Reproduction 80 464-472. (doi:10.1095/biolreprod. 108.073593)
17. Chi MM, Pingsterhaus J, Carayannopoulos M & Moley KH 2000 Decreased glucose transporter expression triggers BAX-dependent apoptosis in the murine blastocyst. Journal of Biological Chemistry 275 40252-40257. (doi:10.1074/jbc.M005508200)
18. Chiaratti MR, Ferreira CR, Meirelles FV, Meo SC, Perecin F, Smith LC, Ferraz ML, de Sa Filho MF, Gimenes LU, Baruselli PS et al. 2010 Xenooplasmic transfer between buffalo and bovine enables development of homoplasmic offspring. Cellular Reprogramming 12 231-236. (doi:10.1089=cell.2009.0076)
19. Choi I & Campbell KH 2010 Treatment of ovine oocytes with caffeine increases the accessibility of DNase I to the donor chromatin and reduces apoptosis in somatic cell nuclear transfer embryos. Reproduction, Fertility and Development 22 1000-1014. (doi:10.1071/RD09144)
20. Choi I, Lee JH, Fisher P & Campbell KH 2010 Caffeine treatment of ovine cytoplasts regulates gene expression and foetal development of embryos produced by somatic cell nuclear transfer. Molecular Reproduction and Development 77 876-887. (doi:10.1002/mrd.21230)
21. Chung Y, Bishop CE, Treff NR, Walker SJ, Sandler VM, Becker S, Klimanskaya I, Wun WS, Dunn R, Hall RM et al. 2009 Reprogramming of human somatic cells using human and animal oocytes. Cloning Stem Cells 11 213-223. (doi:10.1089/clo.2009.0004)
22. Cibelli JB, Grant KA, Chapman KB, Cunniff K, Worst T, Green HL, Walker SJ, Gutin PH, Vilner L, Tabar V et al. 2002 Parthenogenetic stem cells in nonhuman primates. Science 295 819. (doi:10.1126/science.1065637)
23. Dominko T, Mitalipova M, Haley B, Beyhan Z, Memili E, McKusick B & First NL 1999 Bovine oocyte cytoplasm supports development of embryos produced by nuclear transfer of somatic cell nuclei from various mammalian species. Biology of Reproduction 60 1496-1502. (doi:10.1095/biolreprod60.6.1496)
24. Exley GE, Tang C, McElhinny AS & Warner CM 1999 Expression of caspase and BCL-2 apoptotic family members in mouse preimplantation embryos. Biology of Reproduction 61 231-239. (doi:10.1095/biolreprod61.1.231)
25. Facucho-Oliveira JM, Alderson J, Spikings EC, Egginton S & St John JC 2007 Mitochondrial DNA replication during differentiation of murine embryonic stem cells. Journal of Cell Science 120 4025-4034. (doi:10.1242/jcs.016972)
26. Gomez MC, Jenkins JA, Giraldo A, Harris RF, King A, Dresser BL & Pope CE 2003 Nuclear transfer of synchronized african wild cat somatic cells into enucleated domestic cat oocytes. Biology of Reproduction 69 1032-1041. (doi:10.1095/biolreprod.102.014449)
27. Gomez MC, Pope CE, Ricks DM, Lyons J, Dumas C & Dresser BL 2009 Cloning endangered felids using heterospecific donor oocytes and interspecies embryo transfer. Reproduction, Fertility and Development 21 76-82. (doi:10.1071/RD08222)
28. Hamatani T, Carter MG, Sharov AA & Ko MS 2004 Dynamics of global gene expression changes during mouse preimplantation development. Developmental Cell 6 117-131. (doi:10.1016/S1534-5807(03)00373-3)
29. Home P, Ray S, Dutta D, Bronshteyn I, Larson M & Paul S 2009 GATA3 is selectively expressed in the trophectoderm of peri-implantation embryo and directly regulates Cdx2 gene expression. Journal of Biological Chemistry 284 28729-28737. (doi:10.1074/jbc.M109.016840)
30. Illmensee K, Levanduski M & Zavos PM 2006 Evaluation of the embryonic preimplantation potential of human adult somatic cells via an embryo interspecies bioassay using bovine oocytes. Fertility and Sterility 85 (Supplement 1) 1248-1260. (doi:10.1016/j.fertnstert.2005.10.026)
31. Jurisicova A, Latham KE, Casper RF, Casper RF & Varmuza SL 1998 Expression and regulation of genes associated with cell death during murine preimplantation embryo development. Molecular Reproduction and Development 51 243-253. (doi:10.1002/(SICI)1098-2795(199811)51:3〈243::AID-MRD3〉3.0. CO;2-P)
32. Kaufman MH, Robertson EJ, Handyside AH & EvansMJ 1983 Establishment of pluripotential cell lines from haploid mouse embryos. Journal of Embryology and Experimental Morphology 73 249-261.
33. Kelly RD, Alberio R & Campbell KH 2010 A-type lamin dynamics in bovine somatic cell nuclear transfer embryos. Reproduction, Fertility and Development 22 956-965. (doi:10.1071/RD09264)
34. Kenyon L&Moraes CT 1997 Expanding the functional human mitochondrial DNA database by the establishment of primate xenomitochondrial cybrids. PNAS 94 9131-9135. (doi:10.1073/pnas.94.17.9131)
35. Kim K, Ng K, Rugg-Gunn PJ, Shieh JH, Kirak O, Jaenisch R, Wakayama T, Moore MA, Pedersen RA & Daley GQ 2007 Recombination signatures distinguish embryonic stem cells derived by parthenogenesis and somatic cell nuclear transfer. Cell Stem Cell 1 346-352. (doi:10.1016/j.stem.2007.07.001)
36. Kuckenberg P, Buhl S, Woynecki T, van Furden B, Tolkunova E, Seiffe F, Moser M, Tomilin A, Winterhager E & Schorle H 2010 The transcription factor TCFAP2C/AP-2gamma cooperates with CDX2 to maintain trophectoderm formation. Molecular and Cellular Biology 30 3310-3320. (doi:10.1128/MCB.01215-09)
37. Kwon OY & Kono T 1996 Production of identical sextuplet mice by transferring metaphase nuclei from four-cell embryos. PNAS 93 13010-13013. (doi:10.1073/pnas.93.23.13010)
38. Lagutina I, Lazzari G, Duchi R, Turini P, Tessaro I, Brunetti D, Colleoni S, Crotti G & Galli C 2007 Comparative aspects of somatic cell nuclear transfer with conventional and zona-free method in cattle, horse, pig and sheep. Theriogenology 67 90-98. (doi:10.1016/j.theriogenology.2006.09.011)
39. Lagutina I, Fulka H, Brevini TA, Antonini S, Brunetti D, Colleoni S, Gandolfi F, Lazzari G, Fulka J Jr & Galli C 2010 Development, embryonic genome activity and mitochondrial characteristics of bovine-pig inter-family nuclear transfer embryos. Reproduction 140 273-285. (doi:10.1530/REP-09-0578)
40. Lanza RP, Cibelli JB, Diaz F, Moraes CT, Farin PW, Farin CE, Hammer CJ, West MD & Damiani P 2000 Cloning of an endangered species (Bos gaurus) using interspecies nuclear transfer. Cloning 2 79-90. (doi:10.1089/152045500436104)
41. Leese HJ & Barton AM1984 Pyruvate and glucose uptake by mouse ova and preimplantation embryos. Journal of Reproduction and Fertility 72 9-13. (doi:10.1530/jrf.0.0720009)
42. Li Y, Dai Y, Du W, Zhao C, Wang H, Wang L, Li R, Liu Y, Wan R & Li N 2006 Cloned endangered species takin (Budorcas taxicolor) by inter-species nuclear transfer and comparison of the blastocyst development with yak (Bos grunniens) and bovine. Molecular Reproduction and Development 73 189-195. (doi:10.1002/mrd.20405)
43. Liang CG, Han Z, Cheng Y, Zhong Z & Latham KE 2009 Effects of ooplasm transfer on paternal genome function in mice. Human Reproduction 24 2718-2728. (doi:10.1093/humrep/dep286)
44. Liu SZ, Zhou ZM, Chen T, Zhang YL, Wen DC, Kou ZH, Li ZD, Sun QY & Chen DY 2004 Blastocysts produced by nuclear transfer between chicken blastodermal cells and rabbit oocytes. Molecular Reproduction and Development 69 296-302. (doi:10.1002/mrd.20091)
45. Loi P, Ptak G, Barboni B, Fulka J Jr, Cappai P & Clinton M 2001 Genetic rescue of an endangered mammal by cross-species nuclear transfer using post-mortem somatic cells. Nature Biotechnology 19 962-964. (doi:10.1038/ nbt1001-962)
46. Mason IL 1976 Genetics. In The Husbandry and Health of Domestic Buffalo, pp 57-81. Ed W Ross Cockrill. Rome: FAO.
47. McKenzie M & Trounce I 2000 Expression of Rattus norvegicus mtDNA in Mus musculus cells results in multiple respiratory chain defects. Journal of Biological Chemistry 275 31514-31519. (doi:10.1074/jbc.M004070200)
48. McKenzie M, Chiotis M, Pinkert CA & Trounce IA 2003 Functional respiratory chain analyses in murid xenomitochondrial cybrids expose coevolutionary constraints of cytochrome b and nuclear subunits of complex III. Molecular Biology and Evolution 20 1117-1124. (doi:10.1093/molbev/msg132)
49. Meirelles FV, Bordignon V, Watanabe Y, Watanabe M, Dayan A, Lobo RB, Garcia JM & Smith LC 2001 Complete replacement of the mitochondrial genotype in a Bos indicus calf reconstructed by nuclear transfer to a Bos taurus oocyte. Genetics 158 351-356. (Pubitemid 32428655)
50. Moley KH, Chi MM, Knudson CM, Korsmeyer SJ & Mueckler MM 1998 Hyperglycemia induces apoptosis in pre-implantation embryos through cell death effector pathways. Nature Medicine 4 1421-1424. (doi:10.1038/4013)
51. Nishioka N, Yamamoto S, Kiyonari H, Sato H, Sawada A, Ota M, Nakao K & Sasaki H 2008 Tead4 is required for specification of trophectoderm in pre-implantation mouse embryos. Mechanisms of Development 125 270-283. (doi:10.1016/j.mod.2007.11.002)
52. Niwa H, Toyooka Y, Shimosato D, Strumpf D, Takahashi K, Yagi R & Rossant J 2005 Interaction between Oct3/4 and Cdx2 determines trophectoderm differentiation. Cell 123 917-929. (doi:10.1016/j.cell.2005.08.040)
53. Oback B, Wiersema AT, Gaynor P, Laible G, Tucker FC, Oliver JE, Miller AL, Troskie HE, Wilson KL, Forsyth JT et al. 2003 Cloned cattle derived from a novel zona-free embryo reconstruction system. Cloning Stem Cells 5 3-12. (doi:10.1089/153623003321512111)
54. Oh B, Hwang S, McLaughlin J, Solter D & Knowles BB 2000 Timely translation during the mouse oocyte-to-embryo transition. Development 127 3795-3803.
55. Oh BC, Kim JT, Shin NS, Kwon SW, Kang SK, Lee BC & Hwang WS 2006 Production of blastocysts after intergeneric nuclear transfer of goral (Naemorhedus goral) somatic cells into bovine oocytes. Journal of Veterinary Medical Science 68 1167-1171. (doi:10.1292/jvms.68.1167)
56. Ostertag EM & Kazazian HH Jr 2001 Biology of mammalian L1 retrotransposons. Annual Review of Genetics 35 501-538. (doi:10.1146/annurev. genet.35.102401.091032)
57. Pantaleon M & Kaye PL 1998 Glucose transporters in preimplantation development. Reviews of Reproduction 3 77-81. (doi:10.1530/ror.0.0030077)
58. Pantaleon M, Harvey MB, Pascoe WS, James DE & Kaye PL 1997 Glucose transporter GLUT3: ontogeny, targeting, and role in the mouse blastocyst. PNAS 94 3795-3800. (doi:10.1073/pnas.94.8.3795)
59. Piko L & Matsumoto L 1976 Number of mitochondria and some properties of mitochondrial DNA in the mouse egg. Developmental Biology 49 1-10. (doi:10.1016/0012-1606(76)90253-0)
60. Piko L & Taylor KD 1987 Amounts of mitochondrial DNA and abundance of some mitochondrial gene transcripts in early mouse embryos. Developmental Biology 123 364-374. (doi:10.1016/0012-1606(87)90395-2)
61. Ralston A, Cox BJ, Nishioka N, Sasaki H, Chea E, Rugg-Gunn P, Guo G, Robson P, Draper JS & Rossant J 2010 Gata3 regulates trophoblast development downstream of Tead4 and in parallel to Cdx2. Development 137 395-403. (doi:10.1242/dev.038828)
62. Sansinena MJ, Lynn J, Bondioli KR, Denniston RS & Godke RA 2011 Ooplasm transfer and interspecies somatic cell nuclear transfer: heteroplasmy, pattern of mitochondrial migration and effect on embryo development. Zygote 19 147-156. (doi:10.1017/S0967199410000419)
63. Saunders LR & Barber GN 2003 The dsRNA binding protein family: critical roles, diverse cellular functions. FASEB Journal 17 961-983. (doi:10.1096/fj.02-0958rev)
64. Schmittgen TD & Livak KJ 2008 Analyzing real-time PCR data by the comparative C(T) method. Nature Protocols 3 1101-1108. (doi:10.1038/nprot.2008. 73)
65. Sturmey RG & Leese HJ 2003 Energy metabolism in pig oocytes and early embryos. Reproduction 126 197-204. (doi:10.1530/rep.0.1260197)
66. Thundathil J, Filion F & Smith LC 2005 Molecular control of mitochondrial function in preimplantation mouse embryos. Molecular Reproduction and Development 71 405-413. (doi:10.1002/mrd.20260)
67. Trimarchi JR, Liu L, Porterfield DM, Smith PJ & Keefe DL 2000 Oxidative phosphorylation-dependent and -independent oxygen consumption by individual preimplantation mouse embryos. Biology of Reproduction 62 1866-1874. (doi:10.1095/biolreprod62.6.1866)
68. Trounce IA & Pinkert CA 2007 Cybrid models of mtDNA disease and transmission, from cells to mice. Current Topics in Developmental Biology 77 157-183. (doi:10.1016/S0070-2153(06)77006-5)
69. Van Blerkom J, Davis P & Alexander S 2000 Differential mitochondrial distribution in human pronuclear embryos leads to disproportionate inheritance between blastomeres: relationship to microtubular organization, ATP content and competence. Human Reproduction 15 2621-2633. (doi:10.1093/humrep/15.12.2621)
70. Van Thuan N, Wakayama S, Kishigami S, Ohta H, Hikichi T, Mizutani E, Bui HT & Wakayama T 2006 Injection of somatic cell cytoplasm into oocytes before intracytoplasmic sperm injection impairs full-term development and increases placental weight in mice. Biology of Reproduction 74 865-873. (doi:10.1095/biolreprod.105.047803)
71. Wakayama T, Perry AC, Zuccotti M, Johnson KR & Yanagimachi R 1998 Full-term development of mice from enucleated oocytes injected with cumulus cell nuclei. Nature 394 369-374. (doi:10.1038/28615)
72. Wang K, Beyhan Z, Rodriguez RM, Ross PJ, Iager AE, Kaiser GG, Chen Y & Cibelli JB 2009 Bovine ooplasm partially remodels primate somatic nuclei following somatic cell nuclear transfer. Cloning Stem Cells 11 187-202. (doi:10.1089/clo.2008.0061)
73. Watson AJ & Barcroft LC 2001 Regulation of blastocyst formation. Frontiers in Bioscience 6 D708-D730. (doi:10.2741/Watson)
74. Wen DC, Bi CM, Xu Y, Yang CX, Zhu ZY, Sun QY & Chen DY 2005 Hybrid embryos produced by transferring panda or cat somatic nuclei into rabbit MII oocytes can develop to blastocyst in vitro. Journal of Experimental Zoology. Part A, Comparative Experimental Biology 303 689-697. (doi:10.1002/jez.a.191)
75. Yagi R, Kohn MJ, Karavanova I, Kaneko KJ, Vullhorst D, DePamphilis ML & Buonanno A 2007 Transcription factor TEAD4 specifies the trophectoderm lineage at the beginning of mammalian development. Development 134 3827-3836. (doi:10.1242/dev.010223)
76. Yamanaka Y, Ralston A, Stephenson RO & Rossant J 2006 Cell and molecular regulation of the mouse blastocyst. Developmental Dynamics 235 2301-2314. (doi:10.1002/dvdy.20844)
77. Yang CX, Han ZM, Wen DC, Sun QY, Zhang KY, Zhang LS, Wu YQ, Kou ZH & Chen DY 2003 In vitro development and mitochondrial fate of macaca-rabbit cloned embryos. Molecular Reproduction and Development 65 396-401. (doi:10.1002/mrd.10320)
78. Yin XJ, Lee Y, Lee H, Kim N, Kim L, Shin H & Kong I 2006 In vitro production and initiation of pregnancies in inter-genus nuclear transfer embryos derived from leopard cat (Prionailurus bengalensis) nuclei fused with domestic cat (Felis silverstris catus) enucleated oocytes. Theriogenology 66 275-282. (doi:10.1016/j.theriogenology.2005.11.016)
79. Yu J, Hecht RM & Schultz RM 2001 Expression of MSY2 in mouse oocytes and preimplantation embryos. Biology of Reproduction 65 1260-1270. (doi:10.1095/biolreprod65.4.1260)
80. Zhao ZJ, Li RC, Cao HH, Zhang QJ, Jiang MX, Ouyang YC, Nan CL, Lei ZL, Song XF, Sun QY et al. 2007 Interspecies nuclear transfer of Tibetan antelope using caprine oocyte as recipient. Molecular Reproduction and Development 74 412-419. (doi:10.1002/mrd.20608)