Viability of respiratory pathogens cultured from nasopharyngeal swabs stored for up to 12years at -70°C in skim milk tryptone glucose glycerol broth

Journal of Microbiological Methods

Volumn 86, Issue 3, 2011, Pages 364-367

  Hare, Kim M ^a   Smith Vaughan, Heidi C ^a   Leach, Amanda J ^a  

^a MENZIES SCHOOL OF HEALTH RESEARCH   (Australia)

Author keywords

Long term viability; Nasopharyngeal carriage; Respiratory pathogens; Ultra freeze storage

Indexed keywords

GLUCOSE; GLYCEROL;

ARTICLE; BACTERIAL COUNT; BACTERIAL VIABILITY; BACTERIUM CULTURE; BACTERIUM DETECTION; CULTURE MEDIUM; FREEZING; HAEMOPHILUS INFLUENZAE; MILK; MORAXELLA CATARRHALIS; NONHUMAN; NOSE SMEAR; PRIORITY JOURNAL; STORAGE TEMPERATURE; STREPTOCOCCUS PNEUMONIAE; THAWING; THROAT CULTURE;

BACTERIOLOGICAL TECHNIQUES; CULTURE MEDIA; FREEZING; HAEMOPHILUS INFLUENZAE; HUMANS; MICROBIAL VIABILITY; MORAXELLA (BRANHAMELLA) CATARRHALIS; NASAL MUCOSA; NASOPHARYNX; SPECIMEN HANDLING; STREPTOCOCCUS PNEUMONIAE; TIME FACTORS;

BACTERIA (MICROORGANISMS); HAEMOPHILUS INFLUENZAE; MORAXELLA CATARRHALIS; STREPTOCOCCUS PNEUMONIAE;

EID: 79960627938     PISSN: 01677012     EISSN: 18728359     Source Type: Journal    
DOI: 10.1016/j.mimet.2011.06.016     Document Type: Article

References (16)

1. Aulet de Saab O.C., de Castillo M.C., Ruiz Holgado A.P., de Nader O.M. A comparative study of preservation and storage of Haemophilus influenzae. Mem. Inst. Oswaldo Cruz. 2001, 96:583-586.
2. Bailie R., Morris P.S., Leach A.J., Wilson C., Dunne K., Si D., Stubbs L., Smith-Vaughan H.C., Skull S., Mathews C., Mathews J.D. The effect of additional training in recommended hygiene practices on rates of bacterial cross-infection and respiratory illness in Australian child care centres: a randomised controlled trial. Epidemiology 2003, 14:S15.
3. Binks M., Cheng A., Smith-Vaughan H., Sloots T., Nissen M., Whiley D., McDonnell J., Leach A.J. Viral-bacterial co-infection in Australian indigenous children with otitis media. BMC Infect, Dis. 2011, 11:161.
4. Gibson L., Khoury J. Storage and survival of bacteria by ultra-freeze. Lett. Appl. Microbiol. 1986, 3:127-129.
5. Hare K.M., Stubbs E., Beissbarth J., Morris P.S., Leach A.J. Swab transport in Amies gel followed by frozen storage in skim milk tryptone glucose glycerol broth (STGGB) for studies of respiratory bacterial pathogens. J. Microbiol. Methods 2010, 81:253-255.
6. Hare K.M., Grimwood K., Leach A.J., Smith-Vaughan H., Torzillo P.J., Morris P.S., Chang A.B. Respiratory bacterial pathogens in the nasopharynx and lower airways of Australian indigenous children with bronchiectasis. J. Pediatr. 2010, 157:1001-1005.
7. Kaijalainen T., Ruokokoski E., Ukkonen P., Herva E. Survival of Streptococcus pneumoniae, Haemophilus influenzae, and Moraxella catarrhalis frozen in skim milk-tryptone-glucose-glycerol medium. J. Clin. Microbiol. 2004, 42:412-414.
8. Leach A.J., Boswell J.B., Asche V., Nienhuys T.G., Mathews J.D. Bacterial colonization of the nasopharynx predicts very early onset and persistence of otitis media in Australian aboriginal infants. Pediatr. Infect. Dis. J. 1994, 13:983-989.
9. Leach A.J., Morris P.S., Mathews J.D. Compared to placebo, long-term antibiotics resolve otitis media with effusion (OME) and prevent acute otitis media with perforation (AOMwiP) in a high-risk population: a randomized controlled trial. BMC Pediatr. 2008, 8:23.
10. Mackenzie G.A., Carapetis J.R., Leach A.J., Morris P.S. Pneumococcal vaccination and otitis media in Australian Aboriginal infants: comparison of two birth cohorts before and after introduction of vaccination. BMC Pediatr. 2009, 9:14.
11. Moore H.C., Jacoby P., Taylor A., Harnett G., Bowman J., Riley T.V., Reuter K., Smith D.W., Lehmann D. The interaction between respiratory viruses and pathogenic bacteria in the upper respiratory tract of asymptomatic Aboriginal and non-Aboriginal children. Pediatr. Infect. Dis. J. 2010, 29:540-545.
12. O'Brien K.L., Bronsdon M.A., Dagan R., Yagupsky P., Janco J., Elliott J., Whitney C.G., Yang Y.H., Robinson L.G., Schwartz B., Carlone G.M. Evaluation of a medium (STGG) for transport and optimal recovery of Streptococcus pneumoniae from nasopharyngeal secretions collected during field studies. J. Clin. Microbiol. 2001, 39:1021-1024.
13. O'Brien K.L., Nohynek H. Report from a WHO Working Group: standard method for detecting upper respiratory carriage of Streptococcus pneumoniae. Pediatr. Infect. Dis. J. 2003, 22:e1-e11.
14. Siberry G., Brahmadathan K.N., Pandian R., Lalitha M.K., Steinhoff M.C., John T.J. Comparison of different culture media and storage temperatures for the long-term preservation of Streptococcus pneumoniae in the tropics. Bull. World Health Organ. 2001, 79:43-47.
15. Smith-Vaughan H., Byun R., Nadkarni M., Jacques N.A., Hunter N., Halpin S., Morris P.S., Leach A.J. Measuring nasal bacterial load and its association with otitis media. BMC Ear Nose Throat Disord. 2006, 6:10.
16. Smith-Vaughan H., Byun R., Halpin S., Nadkarni M.A., Jacques N.A., Hunter N., Morris P.S., Leach A.J. Interventions for prevention of otitis media may be most effective if implemented in the first weeks of life. Int. J. Pediatr. Otorhinolaryngol. 2008, 72:57-61.