A quantitative TaqMan PCR assay for the detection of Mycoplasma suis

Journal of Applied Microbiology

Volumn 111, Issue 2, 2011, Pages 417-425

  Guimaraes, A M S ^a,b   Vieira, R F C ^c   Poletto, R ^d   Vemulapalli, R ^a   Santos, A P ^a   de Moraes, W ^e   Cubas, Z S ^e   Santos, L C ^e   Marchant Forde, J N ^d   Timenetsky, J ^f   Biondo, A W ^c,g   Messick, J B ^a  

^a PURDUE UNIVERSITY   (United States)

^b Ministry of Education Brazil   (Brazil)

^c FEDERAL UNIVERSITY OF PARANÁ   (Brazil)

^d U S DEPARTMENT OF AGRICULTURE   (United States)

^e ITAIPU Binacional   (Brazil)

^f UNIVERSITY OF SÃO PAULO   (Brazil)

^g PURDUE UNIVERSITY   (United States)

Author keywords

Mycoplasma suis; Peccaries; Pigs; Quantitative PCR; Real time PCR; Swine

Indexed keywords

BLOOD; MAMMALS; POLYMERASE CHAIN REACTION; RNA;

DETECTION AND QUANTIFICATIONS; MYCOPLASMA SUI; PCR ASSAY; PECCARY; PIG; PROBE-BASED; QUANTITATIVE PCR; REAL-TIME PCR; SWINE; TAQMAN PROBES;

PROBES;

RNA 16S;

BACTERIUM; BIOASSAY; BLOOD; MOLECULAR ANALYSIS; PIG; PLASMID; POLYMERASE CHAIN REACTION; PROBE; QUANTITATIVE ANALYSIS; REAL TIME;

ANIMAL EXPERIMENT; ANIMAL MODEL; ARTICLE; BACTERIAL INFECTION; BACTERIUM IDENTIFICATION; BLOOD ANALYSIS; CLONING; CONTROLLED STUDY; CROSS REACTION; DNA SEQUENCE; FEMALE; MOLECULAR PROBE; MYCOPLASMA SUIS; NONHUMAN; PLASMID; POLYMERASE CHAIN REACTION; QUANTITATIVE ANALYSIS; SENSITIVITY AND SPECIFICITY; SWINE;

MYCOPLASMA SUIS; MYCOPLASMATALES; SUIDAE; TAYASSUIDAE;

EID: 79960563540     PISSN: 13645072     EISSN: 13652672     Source Type: Journal    
DOI: 10.1111/j.1365-2672.2011.05053.x     Document Type: Article

References (32)

1. Applied Biosystems (2003). Creating standard curves with genomic DNA or plasmid DNA templates for use in quantitative PCR. Available from URL:, accessed on November 2007.
2. Brownback, A. (1981) Eperythrozoonosis as a cause of infertility in swine. Vet Med Small Anim Clin 76, 375-378.
3. Decaro, N., Elia, G., Martella, V., Campolo, M., Desario, C., Camero, M., Cirone, F., Lorusso, E. et al. (2006) Characterisation of the canine parvovirus type 2 variants using minor groove binder probe technology. J Virol Methods 133, 92-99.
4. Dusanic, D., Bercic, R.L., Cizelj, I., Salmic, S., Narat, M. and Bencina, D. (2009) Mycoplasma synoviae invades non-phagocytic chicken cells in vitro. Vet Microbiol 138, 114-119.
5. Fan, H.H., Kleven, S.H. and Jackwood, M.W. (1995) Application of a polymerase chain reaction with arbitrary primers to strain indentification of Mycoplasma gallisepticum. Avian Dis 39, 729-735.
6. Groebel, K., Hoelzle, K., Wittenbrink, M.M., Ziegler, U. and Hoelzle, L.E. (2009) Mycoplasma suis invades porcine erythrocytes. Infect Immun 77, 576-584.
7. Guimaraes, A.M.S., Biondo, A.W., Lara, A.C. and Messick, J.B. (2007a) Exploratory study of Mycoplasma suis (Eperythrozoon suis) on four commercial pig farms in Southern Brazil. Vet Rec 160, 50-53.
8. Guimaraes, A.M.S., Javorouski, M.L., Bonat, M., Lacerda, O., Balbinotti, B., Queiroz, L.G., Timenetsky, J., Biondo, A.W. et al. (2007b) Molecular detection of 'Candidatus Mycoplasma haemominutum' in a lion (Panthera leo) from a Brazilian zoological garden. Rev Inst Med Trop Sao Paulo 49, 195-196.
9. Guimaraes, A.M.S., Santos, A.P., Sanmiguel, P., Walter, T., Timenetsky, J. and Messick, J.B. (2011) Complete genome sequence of Mycoplasma suis and insights in to its biology and adaption to an erythrocyte niche. PLoS ONE 6, e19574.
10. Hannon, P.G., Lochmiller, R.L., Mellen, J.W., Craig, T.M. and Grant, W.E. (1985) Eperythrozoon in captive juvenile collared peccaries in Texas. J Wildl Dis 4, 439-440.
11. Harris, J.M. and Li-Ping, L. (2007) Superfamily Suoidea. In The Evolution of Artiodactyls ed. Prothero, D.R. and Foss, S.E. pp. 130-145 Baltimore, MD: The Johns Hopkins University Press.
12. Henderson, J.P., O'Hagan, J., Hawe, S.M. and Pratt, M.C. (1997) Anaemia and low viability in piglets infected with Eperythrozoon suis. Vet Rec 140, 144-146.
13. Henry, S.C. (1979) Clinical observations on eperythrozoonosis. J Am Vet Med Assoc 174, 601-603.
14. Higgins, M., Hughes, A., Buzzcott, N. and Lown, J. (2004) High-throughput genotyping of human platelet antigens using the 5'-nuclease assay and minor groove binder probe technology. Vox Sang 87, 114-117.
15. Hoelzle, L., Helbling, M., Hoelzle, K., Ritzmann, M., Heinritzi, K. and Wittenbrink, M.M. (2007) First LightCycler real-time PCR assay for the quantitative detection of Mycoplasma suis in clinical samples. J Microbiol Methods 70, 346-354.
16. Hoelzle, K., Engles, M., Krammer, M.M., Wittenbrink, M.M., Dieckmann, S.M. and Hoelzle, L.E. (2010) Occurrence of Mycoplasma suis in wild boars (Sus scrofa L.). Vet Microbiol 143, 405-409.
17. de Kok, J.B., Wiegerinck, E.T., Giesendorf, B.A. and Swinkels, D.W. (2002) Rapid genotyping of single nucleotide polymorphisms using the novel minor groove binding DNA oligonucleotides (MGB probes). Hum Mutat 19, 554-559.
18. Kutyavin, I.V., Afonina, I.S., Mills, A., Gorn, V.V., Lukhtanov, E.A., Belousov, E.S., Singer, M.J., Walburger, D.K. et al. (2000) 3'-minor groove binder-DNA probes increase sequence specificity at PCR extension temperatures. Nucleic Acids Res 28, 655-661.
19. Marques, L.M., Ueno, P.M., Buzinhani, M., Cortez, B.A., Neto, R.L., Yamaguti, M., Oliveira, R.C., Guimaraes, A.M.S. et al. (2010) Invasion of Ureaplasma diversum in Hep-2 cells. BMC Microbiol 10, 83-90.
20. Messick, J.B. (2004) Hemotrophic mycoplasmas (hemoplasmas): a review and new insights into pathogenic potential. Vet Clin Pathol 33, 2-13.
21. Messick, J.B., Berent, L.M. and Cooper, S.K. (1998) Development and evaluation of a PCR-based assay for detection of Haemobartonella felis in cats and differentiation of H. felis from related bacteria by restriction fragment length polymorphism analysis. J Clin Microbiol 36, 462-466.
22. Messick, J.B., Cooper, S.K. and Huntley, M. (1999) Development and evaluation of a polymerase chain reaction assay using the 16S rRNA gene for detection of Eperythrozoon suis infection. J Vet Diagn Invest 11, 229-236.
23. Messick, J.B., Guimaraes, A.M.S., Macieira, D., Timenetsky, J., Vieira, R.F.C. and Biondo, A.W. (2007) A novel hemoplasma in pigs. Vet Pathol 44, 769.
24. Oberst, R.D., Gwaltney, S.M., Hays, M.P., Morgan, S. and Stair, E.L. (1993) Experimental infections and natural outbreaks of eperythrozoonosis in pigs identified by PCR-DNA hybridizations. J Vet Diagn Invest 5, 351-358.
25. Pearson, W.R., Wood, T., Zhang, Z. and Miller, W. (1997) Comparison of DNA sequences with protein sequences. Genomics 46, 24-36.
26. Prullage, J.B., Williams, R.E. and Gaafar, S.M. (1993) On the transmissibility of Eperythrozoon suis by Stomoxys calcitrans and Aedes aegypti. Vet Parasitol 50, 125-135.
27. Ritzmann, M., Grimm, J., Heinritzi, K., Hoelzle, K. and Hoelzle, L.E. (2009) Prevalence of Mycoplasma suis in slaughter pigs, with correlation of PCR results to hematological findings. Vet Microbiol 133, 84-91.
28. Vieira, R.F., Molento, M.B., dos Santos, L.C., Moraes, W., Cubas, Z.S., Santos, A.P., Guimaraes, A.M., Mohamed, A. et al. (2009) Detection of a novel hemoplasma based on 16S rRNA gene DNA in captive and free-ranging capybaras (Hydrochaeris hydrochaeris). Vet Microbiol 139, 410-413.
29. Vieira, R.F., Molento, M.B., Guimaraes, A.M., Santos, A.P., Bonat, M., Javorouski, M.L., Popp, L., Santos, L.C., et al. (2011) Use of a Mycoplasma suis PCR protocol for screening a population of peccaries (Tayassu pecari and Tayassu tajacu). Rev Bras Parasitol Vet 20, 75-77.
30. Wu, J., Yu, J., Song, C., Sun, S. and Wang, Z. (2006) Porcine eperythrozoonosis in China. Ann N Y Acad Sci 1081, 280-285.
31. Yuan, C.L., Liang, A.B., Yao, C.B., Yang, Z.B., Zhu, J.G., Cui, L., Yu, F., Zhu, N.Y. et al. (2009) Prevalence of Mycoplasma suis (Eperythrozoon suis) infection in swine and swine-farm workers in Shanghai, China. Am J Vet Res 70, 890-894.
32. Zinn, G.M., Jesse, G.W. and Dobson, A.W. (1983) Effect of eperythrozoonosis on sow productivity. J Am Vet Med Assoc 182, 369-371.