A rapid chemical method for lysing Arabidopsis cells for protein analysis

Plant Methods

Volumn 7, Issue 1, 2011, Pages

  Tsugama, Daisuke ^a   Liu, Shenkui ^b   Takano, Tetsuo ^a  

^a UNIVERSITY OF TOKYO   (Japan)

^b NORTHEAST FORESTRY UNIVERSITY   (China)

Author keywords

Arabidopsis; Ca2+; Cell wall; Chelate; Detergent; Pectin

Indexed keywords

ARABIDOPSIS;

EID: 79960559365     PISSN: None     EISSN: 17464811     Source Type: Journal    
DOI: 10.1186/1746-4811-7-22     Document Type: Article

References (15)

1. Kushnirov VV. Rapid and reliable protein extraction from yeast. Yeast 2000, 16:857-860. 10.1002/1097-0061(20000630)16:9<857::AID-YEA561>3.0.CO;2-B, 10861908.
2. Matsuo Y, Asakawa K, Toda T, Katayama S. A Rapid Method for Protein Extraction from Fission Yeast. Biosci Biotechnol Biochem 2006, 70:1992-1994. 10.1271/bbb.60087, 16926515.
3. von der Haar T. Optimized Protein Extraction for Quantitative Proteomics of Yeasts. PLoS One 2007, 2:e1078. 10.1371/journal.pone.0001078, 2031916, 17957260.
4. Osumi M. The Ultrastructure of Yeast: Cell Wall Structure and Formation. Micron 1998, 29:207-233. 10.1016/S0968-4328(97)00072-3, 9684351.
5. Carpita NC, Gibeaut DM. Structural models of primary cell walls in flowering plants: consistency of molecular structure with the physical properties of the walls during growth. Plant J 1993, 3:1-30. 10.1111/j.1365-313X.1993.tb00007.x, 8401598.
6. Laemmli UK. Cleavage of structural proteins during the assembly of the head of bacteriophage T4. Nature 1970, 227:680-685. 10.1038/227680a0, 5432063.
7. Klimyuk V, Carroll B, Thomas C, Jones J. Alkali treatment for rapid preparation of plant material for reliable PCR analysis. Plant J 1993, 3:493-494. 10.1111/j.1365-313X.1993.tb00169.x, 8220456.
8. Wang H, Qi M, Cutler A. A simple method of preparing plant samples for PCR. Nucleic Acids Res 1993, 21:4153-4154. 10.1093/nar/21.17.4153, 310032, 8371994.
9. Thomson D, Henry R. Single-step protocol for preparation of plant tissue for analysis by PCR. BioTechniques 1995, 19:394-400.
10. Tsien RY. New Calcium Indicators and Buffers with High Selectivity against Magnesium and Protons: Design, Synthesis, and Properties of Prototype Structures. Biochemistry 1980, 19:2396-2404. 10.1021/bi00552a018, 6770893.
11. Asai T, Tena G, Plotnikova J, Willmann MR, Chiu WL, Gomez-Gomez L, Ausubel FM, Sheen J. MAP kinase signaling cascade in Arabidopsis innate immunity. Nature 2002, 415:977-983. 10.1038/415977a, 11875555.
12. Wang P, Du Y, Li Y, Ren D, Song C-P. Hydrogen Peroxide-Mediated Activation of MAP Kinase 6 Modulates Nitric Oxide Biosynthesis and Signal Transduction in Arabidopsis. Plant Cell 2010, 22:2981-2998. 10.1105/tpc.109.072959, 20870959.
13. Ullah H, Chen J-G, Temple B, Boyes DC, Alonso JM, Davis KR, Ecker JR, Jones AM. The β-subunit of the Arabidopsis G protein negatively regulates auxin-induced cell division and affects multiple developmental processes. Plant Cell 2003, 15:393-409. 10.1105/tpc.006148, 141209, 12566580.
14. Clough SJ, Bent AF. Floral dip: a simplified method for Agrobacterium-mediated transformation of Arabidopsis thaliana. Plant J 1998, 16:735-743. 10.1046/j.1365-313x.1998.00343.x, 10069079.
15. Towbin H, Staehelin T, Gordon J. Electrophoretic transfer of proteins from polyacrylamide gels to nitrocellulose sheets: procedure and some applications. Proc Natl Acad Sci USA 1979, 76:4350-4354. 10.1073/pnas.76.9.4350, 411572, 388439.